Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Nrior, Renner Renner; Inweregbu, Augusta Ogechi

doi:10.9734/jamb/2019/v15i230087

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…Due to these factors, sites with older spills have lesser total petroleum hydrocarbon (TPH) content; lesser TPH content implies higher chances for the proliferation of microorganisms. The result from this study is in agreement with works done by [18][19][20]. Hydrocarbon-degrading microorganisms are ubiquitously distributed in crude oil polluted wetland soil environments.…”

Section: Discussionsupporting

confidence: 92%

Microbiology and Heavy Metal Content of Wetlands Impacted by Crude Oil Pollution in Rivers State, Southern Nigeria

Chibuike

Ogbonna

Olufunmilayo

2021

MRJI

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Wetland soils constitute vast, under-exploited and sometimes undiscovered ecologies in many countries of the World, including Nigeria. A total of 54 wetland soil samples including surface and subsurface soil at depths of 0-15 cm and 15-30 cm were collected using a sterile hand auger for a period of three months between August and October and subjected to standard and analytical microbiological procedures. The wetland soil samples were further subjected to atomic absorption spectroscopy (AAS) to check for presence and concentration of heavy metals. Results obtained showed that apart from heterotrophic bacterial and fungal counts, hydrocarbon utilizing bacteria (HUB) counts were higher in the surface soil ranging from 12.06±3.43bX107 cfu/g at Iwofe to 6.19±2.67aX107 cfu/g at Chokocho while subsurface soil had HUB ranging from 8.91±6.67aX103 cfu/g at Eagle Island to 4.93±3.95aX103cfu/g at Chokocho. Heavy metals such as Fe, Pb, Cd and Ni were recorded in concentrations above FEPA permissible limit in the surface and subsurface soil across the three wetlands. The heavy metal concentration in each wetland however, decreased with an increase in soil depth. According to literatures, elevated levels of heavy metals in soils decrease microbial population, diversity and activities. However, the microbial population in this study increased with increasing heavy metal concentration which indicates that the microbes can tolerate or utilize heavy metals in their systems; as such can be used for bioremediation of heavy metal polluted soils.

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Section: Discussionsupporting

confidence: 92%

Microbiology and Heavy Metal Content of Wetlands Impacted by Crude Oil Pollution in Rivers State, Southern Nigeria

Chibuike

Ogbonna

Olufunmilayo

2021

MRJI

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“…These organisms were isolated from the fresh water sample. The method described by Holt et al [11] and Nrior and Inweregbu [12], was adopted. Pure cultures of the organisms were obtained from inoculation and incubation of water samples using nutrient Agar.…”

Section: Source Of Microorganismsmentioning

confidence: 99%

Comparative Assessment of Biodegradability Potential of Pseudomonas putida and Bacillus amyloliquefaciens on Oil Spill Dispersant (Aquabreak and Teepol) in Freshwater

Yan

Ugboma

Nrior

2023

MRJI

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To compare and assessment of Biodegradability Potential of Pseudomonas putida and Bacillus amyloliquefaciens on oil spill dispersant (Aquabreak and Teepol) in Freshwater. Fresh water sample were collected from Biara, Gokana L.G.A, and were transported to the Microbiology Laboratory of Rivers State University, Port Harcourt, Nigeria for analyses while Oil spill dispersant (OSD/Aquabreak) was purchased from Barker and Hughes, all in Rivers state. Nine experimental set up were carried out using Bacillus and Pseudomonas species as the bio-augmenting organism. Controls were made without organisms. Its bioremediation potential on the pollutants and two types of test organisms were monitored for 28 days at an interval of 7day period. The setup was aerated twice a week to provide more oxygen for the organisms to thrive. Analysis of samples were carried out using standard analytical procedures. The resultsphysiochemical property of the water shows that as follows: pH 6.5, Temperature 30.0 ºC, Electric conductivity 15 µs/cm, Total dissolved solid 7 mg/l ,Chlorine 0.1 mg/l, Bromine 0.2 mg/l, Salinity (0.01 mg/l), Dissolved oxygen 1.5 mg/l , Biological Oxygen Demand 0.3 mg/l, Nitrate 0.01 mg/l, Sulphate 4.18 mg/l Phosphate 0.10 to 1.5mg/l, Total Hydrocarbon content 24mg/l. Percentage (%) Ultimate biodegradability of the two oil spill dispersant OSD/teepol and OSD/aquabreak revealed that control set-up recorded the 79.3 and 86.7 % , bioremediation set-up augmented with Bacillus amyloliquefaciens recorded 93.7 and 99.1% while the set-up augmented with P. putida had 98.0 and 94.7% respectively . It was observed that P. putida degrade Teepol than Bacillus amyloliquefaciens while aquabreak is more degraded by Bacillus amyloliquefaciens than P. putida. Nevertheless both dispersant shown high level of degradability by the test organisms. Therefore It is recommended that oil companies and government parastatals carrying out remediation in the Niger Delta should be encouraged and OSD/Teepol and OSD/aqubreak due to their high biodegradation potential.

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“…[26] Moisture Content [27] Chlorine (Hanna Multipurpose Meter) [28] Bromine (Hanna Multipurpose Meter) [28] Nitrogen (Hanna Multipurpose Meter) [28] Phosphorous (Hanna Multipurpose Meter) [28] Potassium (Hanna Multipurpose Meter) [28]…”

Section: Soilmentioning

confidence: 99%

Seasonal Influence on the Microbiology and Physicochemistry of Crude Oil Polluted Wetlands

Chibuike, P. M.,

Wemedo, S. A.,

Williams J. O.

et al. 2023

SAJRM

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Pollutants such as crude oil spills in an environment, affect the physical, chemical and biological properties of that environment. This study was conducted to determine the effect of seasonal variations on the microbiological and physicochemical characteristics of three crude oil-polluted wetlands in Rivers State, Nigeria. Soil samples were collected at 3 depths (0-15cm, 15-30cm and 30-45cm) with the aid of a hand auger, sampling was done for a period of twelve months covering the rainy (March to August) and dry seasons (September to February). Samples were analyzed for physicochemical characteristics, using standard analytical and microbiological methods. The data obtained was analyzed using the statistical package for social science (SPSS) version 22 and Duncan’s multiple range test was used to separate means where differences occurred. Microbiological analyses showed that significantly higher microbial counts were observed in the dry season than in the rainy season in the wetland samples which could be due to favourable environmental conditions during the period of study. Wetland soil total heterotrophic bacterial (THB) count in the wet season ranged from 1.09x107cfu/g to 10.45x107cfu/g and from 2.43x107cfu/g to 18.56x107cfu/g in the dry season. Total heterotrophic bacteria had the highest population in all soil depths (0-15cm, 15-30cm and 30-45cm) in both seasons. Hydrocarbon-utilizing bacteria such as Bacillus subtilllis, B. rigui, B. flexus, Lysinibacillus macrolides, Staphylococcus aureus, Proteus penneri and fungi such as Aspergillus species, Fusarium species, Mucor species and Rhizopus species were genetically identified in this study. Temperature and other physicochemical parameters in the wetlands were altered due to the variation in season. Higher temperature ranges were observed in the dry season than in the rainy season as seen in Iwofe, temperature ranged from 26.500C (0-15cm) to 28.500C (30-45cm) in the rainy season while in the dry season it ranged from 31.500C (0-15cm) to 33.500C (30-45cm) which was above FEPA permissible limit of 300C for soil temperature. The study showed that season of study as well as crude oil spillage have significant effects on the microbiology and physicochemical characteristics of wetlands and therefore, contribute to microbiological degradation in wetlands.

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Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Cited by 4 publications

References 10 publications

Microbiology and Heavy Metal Content of Wetlands Impacted by Crude Oil Pollution in Rivers State, Southern Nigeria

Microbiology and Heavy Metal Content of Wetlands Impacted by Crude Oil Pollution in Rivers State, Southern Nigeria

Comparative Assessment of Biodegradability Potential of Pseudomonas putida and Bacillus amyloliquefaciens on Oil Spill Dispersant (Aquabreak and Teepol) in Freshwater

Seasonal Influence on the Microbiology and Physicochemistry of Crude Oil Polluted Wetlands

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