Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent

Oaikhena, Enimie Endurance

doi:10.11648/j.ajep.20160502.12

Cited by 28 publications

(6 citation statements)

References 10 publications

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“…The pure cultures of strains AQ5-15 and AQ5-06 could tolerate Ag concentrations up to 0.4 and 0.6 ppm, respectively, whereas the binary mixture could only tolerate up to 0.2 ppm, at which concentration phenol degradation was already reduced to half of the control level. This result contrasts with a number of studies reporting greater resistance of mixed microbial cultures compared with pure cultures towards toxic heavy metals [74,75]. However, this mixture showed increased tolerance to higher concentrations of Hg and Cd than did pure culture of strain AQ5-15, possibly suggesting that strain AQ5-06 may have a positive synergistic impact on its co-culture's tolerance.…”

Section: Binary Consortiumcontrasting

confidence: 90%

Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria

et al. 2021

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Since the heroic age of Antarctic exploration, the continent has been pressurized by multiple anthropogenic activities, today including research and tourism, which have led to the emergence of phenol pollution. Natural attenuation rates are very slow in this region due to the harsh environmental conditions; hence, biodegradation of phenol using native bacterial strains is recognized as a sustainable remediation approach. The aim of this study was to analyze the effectiveness of phenol degradation by a binary consortium of Antarctic soil bacteria, Arthrobacter sp. strain AQ5-06, and Arthrobacter sp. strain AQ5-15. Phenol degradation by this co-culture was statistically optimized using response surface methodology (RSM) and tolerance of exposure to different heavy metals was investigated under optimized conditions. Analysis of variance of central composite design (CCD) identified temperature as the most significant factor that affects phenol degradation by this consortium, with the optimum temperature ranging from 12.50 to 13.75 °C. This co-culture was able to degrade up to 1.7 g/L of phenol within seven days and tolerated phenol concentration as high as 1.9 g/L. Investigation of heavy metal tolerance revealed phenol biodegradation by this co-culture was completed in the presence of arsenic (As), aluminum (Al), copper (Cu), zinc (Zn), lead (Pb), cobalt (Co), chromium (Cr), and nickel (Ni) at concentrations of 1.0 ppm, but was inhibited by cadmium (Cd), silver (Ag), and mercury (Hg).

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Section: Binary Consortiumcontrasting

confidence: 90%

Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria

et al. 2021

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“…As in this study, we have isolated and characterized an indigenous Staphylococcus aureus strain K1 from metal polluted environment that can tolerate up to 22 mM of Cr 6+ . Several studies had reported the similar results in which metal tolerant bacteria were isolated from metal contaminated habitats (Shakoori, Makhdoom & Haq, 2000; Camargo et al, 2003; Viti, Pace & Giovannetti, 2003; Thacker et al, 2006; Wang et al, 2010; Alam & Ahmad, 2011; Zhang et al, 2013; Khan et al, 2015; Oaikhena et al., 2016). Our results also corroborated the findings of Mustapha & Halimoon (2015) who isolated 21 bacteria from electroplating industry and reported only five of them to be chromium tolerant (up to 50 mg/L).…”

Section: Discussionmentioning

confidence: 54%

Isolation and molecular characterization of the indigenous Staphylococcus aureus strain K1 with the ability to reduce hexavalent chromium for its application in bioremediation of metal-contaminated sites

Tariq

Waseem

Rasool

et al. 2019

PeerJ

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Background Urbanization and industrialization are the main anthropogenic activities that are adding toxic heavy metals to the environment. Among these, chromium (in hexavalent: Cr+6 and/or trivalent Cr+3) is being released abundantly in wastewater due to its uses in different industrial processes. It becomes highly mutagenic and carcinogenic once it enters the cell through sulfate uptake pathways after interacting with cellular proteins and nucleic acids. However, Cr+6 can be bio-converted into more stable, less toxic and insoluble trivalent chromium using microbes. Hence in this study, we have made efforts to utilize chromium tolerant bacteria for bio-reduction of Cr+6 to Cr+3. Methods Bacterial isolate, K1, from metal contaminated industrial effluent from Kala Shah Kaku-Lahore Pakistan, which tolerated up to 22 mM of Cr6+ was evaluated for chromate reduction. It was further characterized biochemically and molecularly by VITEK®2 system and 16S rRNA gene sequencing respectively. Other factors affecting the reduction of chromium such as initial chromate ion concentration, pH, temperature, contact-time were also investigated. The role of cellular surface in sorption of Cr6+ ion was analyzed by FTIR spectroscopy. Results Both biochemical and phylogenetic analyses confirmed that strain K1 was Staphylococcusaureus that could reduce 99% of Cr6+ in 24 hours at 35 °C (pH = 8.0; initial Cr6+ concentration = 100 mg/L). FTIR results assumed that carboxyl, amino and phosphate groups of cell wall were involved in complexation with chromium. Our results suggested that Staphylococcusaureus K1 could be a promising gram-positive bacterium that might be utilized to remove chromium from metal polluted environments.

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“…Cadmium was a naturally occurring heavy metal and a potential bacterial toxicant having an antagonistic effect. Studies by Oaikhena et al [ 60 ] had reported the tolerance of some bacterial isolates to a maximum of 900 μg/l of cadmium. The findings in this research are also similar to those of Smritha and Usha [ 61 ] who reported similar values in terms of cadmium tolerance by bacterial isolates.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical and molecular characterization of heavy metal–tolerant bacteria isolated from soil of mining sites in Nigeria

Ibrahim

Kawo

Yusuf

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Mining for precious metals is detrimental to the composition of soil structure and microbial diversity distribution and is a health risk to human communities around the affected communities. This study was aimed at determining the physical and chemical characteristics and diversity of bacteria in the soil of local mining sites for biosorption of heavy metals. Results Results of physical and chemical characteristics showed mean pH values and percentage organic carbon to range from 7.1 to 8.2 and 0.18 to 1.12% respectively with statistical significance between sampling sites (P ≤ 0.05). Similarly, cation exchange capacity, electrical conductivity, moisture, total nitrogen, and carbon/nitrogen ratio (C:N) in the soil ranged between 1.52 to 3.57 cmol/kg, 0.15 to 0.32 ds/m, 0.14 to 0.82%, 0.10 to 0.28%, and 1.7 to 4.8 respectively. The highest heavy metal concentration of 59.01 ppm was recorded in soils obtained from site 3. The enumeration of viable aerobic bacteria recorded the highest mean count of 4.5 × 106 cfu/g observed at site 2 with statistical significance (P ≤ 0.05) between the sampled soils. Alcaligenes faecalis strain UBI, Aeromonas sp. strain UBI, Aeromonas sobria, and Leptothrix ginsengisoli that make up 11.2% of total identified bacteria were able to grow in higher amended concentrations of heavy metals. The evolutionary relationship showed the four heavy metal–tolerant bacteria identified belonged to the phylum Proteobacteria of class Betaproteobacteria in the order Burkholderiales. Heavy metal biosorption by the bacteria showed Alcaligenes faecalis strain UBI having the highest uptake capacity of 73.5% for Cu. Conclusion In conclusion, Alcaligenes faecalis strain UBI (MT107249) and Aeromonas sp. strain UBI (MT126242) identified in this study showed promising capability to withstand heavy metals and are good candidates in genetic modification for bioremediation.

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Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent

Cited by 28 publications

References 10 publications

Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria

Statistical Assessment of Phenol Biodegradation by a Metal-Tolerant Binary Consortium of Indigenous Antarctic Bacteria

Isolation and molecular characterization of the indigenous Staphylococcus aureus strain K1 with the ability to reduce hexavalent chromium for its application in bioremediation of metal-contaminated sites

Physicochemical and molecular characterization of heavy metal–tolerant bacteria isolated from soil of mining sites in Nigeria

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