Bioresponsive DNA-co-polymer hydrogels for fabrication of sensors

Jonášová, Eleonóra Parelius; Stokke, Bjørn Torger

doi:10.1016/j.cocis.2016.07.001

Cited by 22 publications

(15 citation statements)

References 55 publications

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“…Morpholinos are exhibiting a lack of interactions with protein [50], suggesting they are not suitable for development of protein-binding aptamers and as of today, no Morpholino aptamers have been reported (whether targeting protein or other molecules). However, nucleic acid-based hydrogels can be prepared with a multitude of designs, featuring interactions of several oligonucleotides and other molecules [51]. MOs could be employed in such schemes in hybrid configurations together with DNA strands, where DNA strands provide aptamer functionality and MOs can serve e.g., as blocking strands which are displaced by a biomarker specific for the aptamer.…”

Section: Implications Of Differences Between Mo and Dna Hydrogelsmentioning

confidence: 99%

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Jonášová

Stokke

2020

Polymers

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Responsive hydrogels featuring DNA as a functional unit are attracting increasing interest due to combination of versatility and numerous applications. The possibility to use nucleic acid analogues opens for further customization of the hydrogels. In the present work, the commonly employed DNA oligonucleotides in DNA-co-acrylamide responsive hydrogels are replaced by Morpholino oligonucleotides. The uncharged backbone of this nucleic acid analogue makes it less susceptible to possible enzymatic degradation. In this work we address fundamental issues related to key processes in the hydrogel response; such as partitioning of the free oligonucleotides and the strand displacement process. The hydrogels were prepared at the end of optical fibers for interferometric size monitoring and imaged using confocal laser scanning microscopy of the fluorescently labeled free oligonucleotides to observe their apparent diffusion and accumulation within the hydrogels. Morpholino-based hydrogels’ response to Morpholino targets was compared to DNA hydrogels’ response to DNA targets of the same base-pair sequence. Non-binding targets were observed to be less depleted in Morpholino hydrogels than in DNA hydrogels, due to their electroneutrality, resulting in faster kinetics for Morpholinos. The electroneutrality, however, also led to the total swelling response of the Morpholino hydrogels being smaller than that of DNA, since their lack of charges eliminates swelling resulting from the influx of counter-ions upon oligonucleotide binding. We have shown that employing nucleic acid analogues instead of DNA in hydrogels has a profound effect on the hydrogel response.

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Section: Implications Of Differences Between Mo and Dna Hydrogelsmentioning

confidence: 99%

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Jonášová

Stokke

2020

Polymers

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“…DNA can be served as the only network, building backbone, or versatile linkages for the synthesis of hydrogels . When applied as the only network, DNA gelation occurs based on physical interactions rather than chemical bonds.…”

Section: Dna Hydrogel Fabrication: the Only Network A Backbone Or A mentioning

confidence: 99%

“…Although attempts for the fabrication of DNA hydrogels are still in their early stages, there is a scientific consensus on their affirmative implications to pave the road for further advances on various medical fields, such as drug and gene delivery, biosensing, and tissue engineering . Different methods, including physical entanglement, chemical cross‐linking, and electrostatic interaction with cationic (poly)electrolytes can be used to prepare DNA hydrogels .…”

Section: Introductionmentioning

confidence: 99%

DNA Hydrogel Assemblies: Bridging Synthesis Principles to Biomedical Applications

Shahbazi

Bauleth‐Ramos

Santos

2018

Advanced Therapeutics

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DNA is a perfect polymeric molecule for interfacing biology with material science to construct hydrogels that represent fascinating properties for a wide variety of biomedical applications. Tunable multifunctionality, convenient programmability, adequate biocompatibility, biodegradability, capability of precise molecular recognition, and high versatility have made DNA an irreplaceable building block for the construction of novel 3D hydrogels. DNA can be used as the only component of a hydrogel, the backbone or a cross-linker that connects the main building blocks to form hybrid hydrogels through chemical reactions or physical entanglement. Responsive constructs of DNA with superior mechanical properties are very commonly reported nowadays, which can undergo macroscopic changes induced by various triggers, including alteration in ionic strength, temperature, and pH. These hydrogels can be prepared by various types of DNA building blocks, such as branched double-stranded DNA, single-stranded DNA, X-shaped DNA, or Y-shaped DNA through intermolecular i-motif structures, DNA hybridization, enzyme ligation, or enzyme polymerization. These hydrogels are envisioned for a variety of applications, such as drug delivery, sensing, tissue engineering, 3D cell culture, and providing template for nanoparticle synthesis.

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“…Hydrogels are three dimensional, porous, hydrophilic, physical and chemical crosslinking networks that swell thousands time of their dry weight in the vicinity of water [1]. These materials can respond to specific changes in their environment such as temperature, pH, magnetic field, and the electric field which determines their application in the fields of medicine, tissue engineering [2], wound dressing [3], biosensors [4], pharmaceutical [5], agriculture [6]and adsorption [7]. Hydrogels have found a special place in the field of wastewater treatments.…”

Section: Implementation Of Carboxymethyl Cellulose/acrylic Acid/ Titamentioning

confidence: 99%

Implementation of Carboxymethyl cellulose/Acrylic acid/ Titanium dioxide Nanocomposite Hydrogel in Remediation of Cd(II), Zn(II) and Pb(II) for Water treatment application

Hendy¹,

Khozamy²,

Mahmoud

et al. 2019

Egypt. J. Chem.

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I N THIS study Acrylic acid/ Carboxymethylcellulose/Titanium dioxide (AAc/CMC-TiO 2) hydrogel nanocomposite was synthesized by free-radical crosslinking copolymerization via gamma irradiation. It was characterized using FT-IR and swelling properties. The swelling percentage increases with increasing time until reaches a certain limiting value after 6h. The synthesized nanocomposite was used as an adsorbent for remediation of Cd(II), Zn(II) and Pb(II) from aqueous solutions by the static adsorption technique. It was found that the optimum pH for the adsorption is 5 and the sorption process is carried out at fast operating time, 30-40 min, for all investigated metal ions. It was also found that the metal ions sorption capacity decreases with increasing the dosage of AAc/CMC-TiO 2 from 5 to 150 mg. A progressive increase in the metal ions sorption capacity was obtained with increasing the initial metal ion concentration from 0.01 to 0.05 mol L-1 and the saturation of the sorbent active sites was done at initial metal ions concentration at 0.1 mol L-1. No significant interference on the removal of Pb (II) in the presence of NaCl, Na-acetate, KCl, KNO 3 and NH 4 Cl ions while a slight decrease in removal of Cd (II)and Zn(II) was done. The pseudo-second order kinetic model and Freundlich adsorption model describe well the kinetic and isotherm, respectively, of the adsorption process of the investigated ions.

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Bioresponsive DNA-co-polymer hydrogels for fabrication of sensors

Cited by 22 publications

References 55 publications

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

Morpholino Target Molecular Properties Affect the Swelling Process of Oligomorpholino-Functionalized Responsive Hydrogels

DNA Hydrogel Assemblies: Bridging Synthesis Principles to Biomedical Applications

Implementation of Carboxymethyl cellulose/Acrylic acid/ Titanium dioxide Nanocomposite Hydrogel in Remediation of Cd(II), Zn(II) and Pb(II) for Water treatment application

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