Bioresponsive Surfaces and Interfaces Fabricated by Innovative Laser Approaches

Sima, Félix; Axente, E.; Ristoscu, Carmen; Gallet, Olivier; Anselme, Karine

doi:10.1002/9781119242604.ch12

Cited by 10 publications

(8 citation statements)

References 137 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed protocols for thin coating assembling by MAPLE were addressed elsewhere (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020) and briefly described in the following. The procedure consists of laser irradiation of a cryogenic target by a pulsed UV laser beam (Figure 1).…”

Section: Maple Experimentsmentioning

confidence: 99%

“…Matrix Assisted Pulsed Laser Evaporation (MAPLE) is an additive physical vapor deposition technique developed for functionalization of solid substrates with protein and composite coatings. When compared to other classical deposition methods (e.g., drop-cast, spin-coating, dip-coating, Langmuir-Blodgett), this method provides high experimental versatility and relatively facile control of coating thickness even for ultrathin films, the possibility to preserve materials properties even for very delicate compounds, providing adherent and uniform coatings on centimeter sized substrates, micro-fabrication of multilayers from multi-target system, and possibility to generate gradient coatings in a single-step process (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020). However, current drawbacks are related to difficulties to fabricate large-area coatings (tens of centimeters) with high uniformity.…”

Section: Introductionmentioning

confidence: 99%

“…Laser-assisted synthesis of thin organic coatings of highmolecular mass such as biopolymers, proteins, and enzymes present various technical challenges. In our previous studies (Sima et al, 2016;Axente et al, 2018;Axente and Sima, 2020) we reported synthesis of highly-functional organic, inorganic and hybrid coatings, for biomedical applications. MAPLE technique proved adequate for the delicate laser transfer of biopolymers, as e.g., levan, a high molecular weight, watersoluble bacterial exopolysaccharide (β2,6-linked fructan).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Sima

Chirițoiu²,

Neguț³

et al. 2020

Front. Chem.

View full text Add to dashboard Cite

Since Graphene discovery, their associated derivate nanomaterials, Graphene Oxide (GO) and reduced-GO were in the forefront of continuous developments in bio-nano-technology due to unique physical-chemical properties. Although GO nano-colloids (GON) were proposed as drug release matrix for targeting cancer cells, there is still a concern regarding its cytotoxicity issues. In this study, we report on the fabrication of functional GON bio-coatings by Matrix-Assisted Pulsed Laser Evaporation (MAPLE) to be used as drug carriers for targeting melanoma cells. We first performed a thorough in vitro cytotoxicity assay for comparison between GON and protein functionalized GON coatings. As functionalization protein, Bovine Serum Albumin (BSA) was non-covalently conjugated to GO surface. Safe concentration windows were identified in cytotoxicity tests by live/dead staining and MTS assays for five different human melanoma cell lines as well as for non-transformed melanocytes and human dermal fibroblasts. Hybrid GON-BSA nano-scaled thin coatings incorporating Dabrafenib (DAB) and Trichostatin A (TSA) inhibitors for cells bearing BRAF V600E pathway activating mutation were assembled on solid substrates by MAPLE technique. We further demonstrated the successful immobilization for each drug-containing GON-BSA assembling systems by evaluating cellular BRAF activity inhibition and histone deacetylases activity blocking, respectively. DAB activity was proven by the decreased ERK phosphorylation in primary melanoma cells (SKmel28 BRAF V600E cell line), while TSA effect was evidenced by acetylated histones accumulation in cell's nuclei (SKmel23 BRAF WT cell line). In addition, melanoma cells exposed to GON-BSA coatings with compositional gradient of inhibitors evidenced a dose-dependent effect on target activity. Such functional bio-platforms could present high potential for cell-biomaterial interface engineering to be applied in personalized cancer therapy studies.

show abstract

Section: Maple Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Sima

Chirițoiu²,

Neguț³

et al. 2020

Front. Chem.

View full text Add to dashboard Cite

show abstract

“…They are in the forefront of several discoveries in nanoscience and nanotechnology. Laser-based deposition techniques have been widely employed for the fabrication of various thin biomimetic composite coatings [7,63,[95][96][97][98]. The book edited by Schmidt and Belegratis [99] presents a detailed state-of-the-art of the laser technologies until seven years ago as well as the materials that are typically employed in cutting-edge biomimetic applications.…”

Section: Biomimetic Laser Processingmentioning

confidence: 99%

“…However, there is still a breach in the in-depth understanding of nanomaterial interactions with biological entities both in vitro and in vivo. The design of innovative biomaterials should aim to precisely control the composition-properties relationship in order to modulate cell behavior in the field of tissue engineering and regenerative nanomedicine [7] or cancer theranostics [8].…”

mentioning

confidence: 99%

Biomimetic Coatings Obtained by Combinatorial Laser Technologies

Axente

Sima

2020

Coatings

View full text Add to dashboard Cite

The modification of implant devices with biocompatible coatings has become necessary as a consequence of premature loosening of prosthesis. This is caused mainly by chronic inflammation or allergies that are triggered by implant wear, production of abrasion particles, and/or release of metallic ions from the implantable device surface. Specific to the implant tissue destination, it could require coatings with specific features in order to provide optimal osseointegration. Pulsed laser deposition (PLD) became a well-known physical vapor deposition technology that has been successfully applied to a large variety of biocompatible inorganic coatings for biomedical prosthetic applications. Matrix assisted pulsed laser evaporation (MAPLE) is a PLD-derived technology used for depositions of thin organic material coatings. In an attempt to surpass solvent related difficulties, when different solvents are used for blending various organic materials, combinatorial MAPLE was proposed to grow thin hybrid coatings, assembled in a gradient of composition. We review herein the evolution of the laser technological process and capabilities of growing thin bio-coatings with emphasis on blended or multilayered biomimetic combinations. These can be used either as implant surfaces with enhanced bioactivity for accelerating orthopedic integration and tissue regeneration or combinatorial bio-platforms for cancer research.

show abstract

UV- and RIR-MAPLE: Fundamentals and Applications

Caricato

2018

Advances in the Application of Lasers in Materials Science

View full text Add to dashboard Cite

Bioresponsive Surfaces and Interfaces Fabricated by Innovative Laser Approaches

Cited by 10 publications

References 137 publications

Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Functionalized Graphene Oxide Thin Films for Anti-tumor Drug Delivery to Melanoma Cells

Biomimetic Coatings Obtained by Combinatorial Laser Technologies

UV- and RIR-MAPLE: Fundamentals and Applications

Contact Info

Product

Resources

About