Biorheology of occlusive thrombi formation under high shear: in vitro growth and shrinkage

Rooij, B.J.M. van; Závodszky, Gábor; Hoekstra, Alfons G.; Ku, David N.

doi:10.1038/s41598-020-74518-7

Cited by 18 publications

(8 citation statements)

References 35 publications

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“…Decreased platelet contractility is associated with the abnormal mechanics of blood clots. Several studies have confirmed the high relevance of platelet contractile forces to platelet aggregation and the subsequent hemostasis (Li and Li, 2006;Muthard and Diamond, 2012;Muthard and Diamond, 2013;Ting et al, 2013;Chen Z et al, 2019;van Rooij et al, 2020). Lam et al (2011) customized a side-view atomic force microscope (AFM) to measure the contractile force of a single platelet encapsulated between the fibrinogen-coated cantilever and surface.…”

Section: Microfluidic Devices That Gauge Platelet Contractile Forcementioning

confidence: 97%

Platelet Mechanobiology Inspired Microdevices: From Hematological Function Tests to Disease and Drug Screening

et al. 2022

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Platelet function tests are essential to profile platelet dysfunction and dysregulation in hemostasis and thrombosis. Clinically they provide critical guidance to the patient management and therapeutic evaluation. Recently, the biomechanical effects induced by hemodynamic and contractile forces on platelet functions attracted increasing attention. Unfortunately, the existing platelet function tests on the market do not sufficiently incorporate the topical platelet mechanobiology at play. Besides, they are often expensive and bulky systems that require large sample volumes and long processing time. To this end, numerous novel microfluidic technologies emerge to mimic vascular anatomies, incorporate hemodynamic parameters and recapitulate platelet mechanobiology. These miniaturized and cost-efficient microfluidic devices shed light on high-throughput, rapid and scalable platelet function testing, hematological disorder profiling and antiplatelet drug screening. Moreover, the existing antiplatelet drugs often have suboptimal efficacy while incurring several adverse bleeding side effects on certain individuals. Encouraged by a few microfluidic systems that are successfully commercialized and applied to clinical practices, the microfluidics that incorporate platelet mechanobiology hold great potential as handy, efficient, and inexpensive point-of-care tools for patient monitoring and therapeutic evaluation. Hereby, we first summarize the conventional and commercially available platelet function tests. Then we highlight the recent advances of platelet mechanobiology inspired microfluidic technologies. Last but not least, we discuss their future potential of microfluidics as point-of-care tools for platelet function test and antiplatelet drug screening.

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Section: Microfluidic Devices That Gauge Platelet Contractile Forcementioning

confidence: 97%

Platelet Mechanobiology Inspired Microdevices: From Hematological Function Tests to Disease and Drug Screening

et al. 2022

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“…In a shear gradient context, it was observed that VWF unfolding increased at two-fold lower flow rates when compared to constant shear flows (Zhang, 2009;Sing and Alexander-katz, 2010). Corresponding studies revealed that rapid alterations of the flow through shear microgradients promoted discoid platelet aggregation in the post-stenotic region, or micro-vessel curvatures (Nesbitt et al, 2009;Rooij et al, 2020;Spieker et al, 2021). It suggests that the rapid decrease in shear rate restructures the filamentous membrane tethers and allows integrins α IIb β 3 to considerably stabilise the aggregate.…”

Section: Disturbed and Elongated Shear Rate: Microgradientsmentioning

confidence: 99%

Design of artificial vascular devices: Hemodynamic evaluation of shear-induced thrombogenicity

Feaugas

Newman²,

Calzuola³

et al. 2023

Front. Mech. Eng.

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Blood-circulating devices such as oxygenators have offered life-saving opportunities for advanced cardiovascular and pulmonary failures. However, such systems are limited in the mimicking of the native vascular environment (architecture, mechanical forces, operating flow rates and scaffold compositions). Complications involving thrombosis considerably reduce their implementation time and require intensive anticoagulant treatment. Variations in the hemodynamic forces and fluid-mediated interactions between the different blood components determine the risk of thrombosis and are generally not taken sufficiently into consideration in the design of new blood-circulating devices. In this Review article, we examine the tools and investigations around hemodynamics employed in the development of artificial vascular devices, and especially with advanced microfluidics techniques. Firstly, the architecture of the human vascular system will be discussed, with regards to achieving physiological functions while maintaining antithrombotic conditions for the blood. The aim is to highlight that blood circulation in native vessels is a finely controlled balance between architecture, rheology and mechanical forces, altogether providing valuable biomimetics concepts. Later, we summarize the current numerical and experimental methodologies to assess the risk of thrombogenicity of flow patterns in blood circulating devices. We show that the leveraging of both local hemodynamic analysis and nature-inspired architectures can greatly contribute to the development of predictive models of device thrombogenicity. When integrated in the early phase of the design, such evaluation would pave the way for optimised blood circulating systems with effective thromboresistance performances, long-term implantation prospects and a reduced burden for patients.

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“…Activated platelets in newly formed aggregates are highly contractile with respect to thrombus stabilization and consolidation (76)(77)(78)(79)(80)(81). Recent studies have sought to analyze the degree of forceful cytoskeletal contractions in activated platelets.…”

Section: Platelet Contractionmentioning

confidence: 99%

Emerging Microfluidic Approaches for Platelet Mechanobiology and Interplay With Circulatory Systems

Zhang

Ramasundara

Preketes-Tardiani

et al. 2021

Front. Cardiovasc. Med.

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Understanding how platelets can sense and respond to hemodynamic forces in disturbed blood flow and complexed vasculature is crucial to the development of more effective and safer antithrombotic therapeutics. By incorporating diverse structural and functional designs, microfluidic technologies have emerged to mimic microvascular anatomies and hemodynamic microenvironments, which open the floodgates for fascinating platelet mechanobiology investigations. The latest endothelialized microfluidics can even recapitulate the crosstalk between platelets and the circulatory system, including the vessel walls and plasma proteins such as von Willebrand factor. Hereby, we highlight these exciting microfluidic applications to platelet mechanobiology and platelet–circulatory system interplay as implicated in thrombosis. Last but not least, we discuss the need for microfluidic standardization and summarize the commercially available microfluidic platforms for researchers to obtain reproducible and consistent results in the field.

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Biorheology of occlusive thrombi formation under high shear: in vitro growth and shrinkage

Cited by 18 publications

References 35 publications

Platelet Mechanobiology Inspired Microdevices: From Hematological Function Tests to Disease and Drug Screening

Platelet Mechanobiology Inspired Microdevices: From Hematological Function Tests to Disease and Drug Screening

Design of artificial vascular devices: Hemodynamic evaluation of shear-induced thrombogenicity

Emerging Microfluidic Approaches for Platelet Mechanobiology and Interplay With Circulatory Systems

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