Biosafety assessment of Acinetobacter strains isolated from the Three Gorges Reservoir region in nematode Caenorhabditis elegans

Deng, Yunjia; Du, Huihui; Tang, Ming‐Chun; Wang, Qilong; Huang, Qian; He, Yiliang; Cheng, Fei; Zhao, Feng; Wang, Dayong; Xiao, Guosheng

doi:10.1038/s41598-021-99274-0

Cited by 8 publications

(5 citation statements)

References 55 publications

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“…At the same time, slow-flowing water in the backwater areas of the TGR causes high MP retention. In previous studies, we found that the surface water in backwater areas of the TGR is also favorable to organisms (Xiao et al 2018a ; Xiao et al 2018b ; Deng et al 2021 ).…”

Section: Discussionmentioning

confidence: 92%

Seasonal pollution and surface characteristics of microplastics in surface water in the Wanzhou section of the Three Gorges Reservoir, China

Huang

Wang

et al. 2023

Environ Sci Pollut Res

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The pollution of freshwater environments with microplastics (MPs) has attracted increasing attention owing to their threats to aquatic ecosystems and human health. Here, we sampled and analyzed MPs from mainstream, tributary, and backwater areas in the Wanzhou section of the Three Gorges Reservoir (TGR) in impoundment and flood periods. Microplastic pollution was the most severe in the backwater areas. The average abundance of MPs reached the highest value in the flood period (5.27±3.47×107 items km−2), which was 3–5 times that in the impoundment period. In the 0.3–5 mm size class, the 1–5 mm fraction was the most abundant, accounting for more than 81% in the flood period and 68% of the total MP particle abundance in the impoundment period in the mainstream and backwater areas. However, 0.3–1 mm MPs contributed more than 50% in the tributaries during the impoundment period. Polystyrene, polypropylene, and polyethylene MPs were detected in foam, fragment, sheet, and line-shaped MP particles. White, opaque, foamed polystyrene MPs contributed 32–81% to total MP particle abundance in the watershed. Microplastic particle surfaces showed signs of damage and oxidation, and ten different elements were found. Oxygen was clustered on the surface of foam and fragment MPs. Microplastic pollution was severe in the Wanzhou watershed. Especially in the backwater areas, oxidized MPs of variable shapes derived mainly from surface runoff in the flood period and sewage discharge in the impoundment period were abundant. The results of this study contribute to understanding seasonal pollution patterns and surface characteristics of MPs in the TGR and similar watersheds.

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Section: Discussionmentioning

confidence: 92%

Seasonal pollution and surface characteristics of microplastics in surface water in the Wanzhou section of the Three Gorges Reservoir, China

Huang

Wang

et al. 2023

Environ Sci Pollut Res

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“…AC15 is our isolated Acinetobacter strain in the TGR region [ 23 ]. A. johnsonii AC15 is normally stored in broth glycerol at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In the TGR, plastic pollutions, including the microplastics, have been detected in both sediments and surface water [ 21 , 22 ]. AC15 is an Acinetobacter johnsonii strain isolated from the TGR region [ 23 ]. A. johnsonii is an opportunistic pathogen.…”

Section: Introductionmentioning

confidence: 99%

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Exposure to Nanoplastic Particles Enhances Acinetobacter Survival, Biofilm Formation, and Serum Resistance

Tang

Ding

et al. 2022

Nanomaterials

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The interaction between nanoplastics and bacteria remains still largely unclear. In this study, we determined the effect of nanopolystyrene particle (NP) on a bacterial pathogen of Acinetobacter johnsonii AC15. Scanning electron microscopy (SEM) analysis indicated the aggregation of NPs from 10 μg/L to 100 μg/L on surface of A. johnsonii AC15, suggesting that A. johnsonii AC15 acted as the vector for NPs. Exposure to 100–1000 μg/L NPs increased the growth and colony-forming unit (CFU) of A. johnsonii AC15. In addition, exposure to 100–1000 μg/L NPs enhanced the amount of formed biofilm of A. johnsonii AC15. Alterations in expressions of 3 survival-related (zigA, basD, and zur), 5 biofilm formation-related (ompA, bap, adeG, csuC, and csuD), and 3 serum resistance-related virulence genes (lpxC, lpxL, and pbpG) were observed after exposure to 1000 μg/L NPs. Moreover, both CFU and survival rate of A. johnsonii AC15 in normal human serum (NHS) were significantly increased by 1–1000 μg/L NPs, suggesting the enhancement in serum resistance of Acinetobacter pathogen by NPs. In the NHS, expressions of 3 survival-related (zigA, basD, and zur), 9 biofilm formation-related (ompA, bap, adeF, adeG, csuA/B, csuC, csuD, csuE, and hlyD), and 3 serum resistance-related virulence genes (lpxC, lpxL, and pbpG) were affected by 1000 μg/L NPs. Expressions of 1 survival-related (zigA), 5 biofilm formation-related (bap, adeG, csuC, csuD, and csuE), and 3 serum resistance-related virulence genes (lpxC, lpxL, and pbpG) were also altered by 10 μg/L NPs after the addition of NHS. Therefore, exposure to NPs in the range of μg/L has the potential to enhance bacterial virulence by increasing their growth, biofilm formation, and serum resistance.

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“…Today, there is still a lack of clear evidence regarding the natural habitat of the pathogen outside hospitals, its introduction to hospitals, and any subsequent return to the natural environment. Several studies have documented isolations of A. baumannii from various out-of-hospital habitats, including water bodies [30,31], soil [32,33], food [34,35], meat from food animals [36,37], vegetables [38,39], farm animals [40], domestic animals (cats [41,42], dogs [43,44], and horses [45]), wild animals [46,47], and even body lice [48].…”

Section: Out-of-hospital Habitats For Acinetobacter Baumanniimentioning

confidence: 99%

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

Ahuatzin-Flores,

Torres,

Chávez-Bravo

2024

Microorganisms

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In recent years, humanity has begun to face a growing challenge posed by a rise in the prevalence of antibiotic-resistant bacteria. This has resulted in an alarming surge in fatalities and the emergence of increasingly hard-to-manage diseases. Acinetobacter baumannii can be seen as one of these resilient pathogens due to its increasing prevalence in hospitals, its resistance to treatment, and its association with elevated mortality rates. Despite its clinical significance, the scientific understanding of this pathogen in non-hospital settings remains limited. Knowledge of its virulence factors is also lacking. Therefore, in this review, we seek to shed light on the latest research regarding the ecological niches, microbiological traits, and antibiotic resistance profiles of Acinetobacter baumannii. Recent studies have revealed the presence of this bacterium in a growing range of environmental niches, including rivers, treatment plants, and soils. It has also been discovered in diverse food sources such as meat and vegetables, as well as in farm animals and household pets such as dogs and cats. This broader presence of Acinetobacter baumannii, i.e., outside of hospital environments, indicates a significant risk of environmental contamination. As a result, greater levels of awareness and new preventive measures should be promoted to address this potential threat to public health.

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Biosafety assessment of Acinetobacter strains isolated from the Three Gorges Reservoir region in nematode Caenorhabditis elegans

Cited by 8 publications

References 55 publications

Seasonal pollution and surface characteristics of microplastics in surface water in the Wanzhou section of the Three Gorges Reservoir, China

Seasonal pollution and surface characteristics of microplastics in surface water in the Wanzhou section of the Three Gorges Reservoir, China

Exposure to Nanoplastic Particles Enhances Acinetobacter Survival, Biofilm Formation, and Serum Resistance

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

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