Biosafety Evaluation of Three Sodium Lauryl N‐Amino Acids Synthesized from Silk Industrial Waste in Mice

Abstract

In this study, three amino acid mixtures recovered from silk industry waste were reacted with lauryl chloride to synthesize three sodium laurel N‐amino acid surfactants. The three types of anionic surfactants are referred to as silk fibroin surfactant, silk sericin surfactant, and silkworm pupae surfactant. All three surfactants showed good surface properties. To evaluate biological safety, we added these three surfactants to the normal diet of mice for 8 weeks. Blood indices, blood lipid, antioxidant capacity (GSH‐PX, MDA, T‐AOC, and T‐SOD), and lipid metabolism key enzyme mRNA (PPAR‐γ, SREBP, FASN, and C/EBP‐α) levels in the liver were measured. No evident histopathological changes were found in the liver and kidney. The results showed no significant difference in their indexes between the surfactant‐fed and normal diet‐fed mice. Thus, the three amino acid surfactants are biologically safe and can be used in daily chemical cleaning products. These surfactants will not only help to reduce wastage of resources and damage to the environment, but also yield economic benefits to the silk industry in the future.

Impact of polymorphism in oleogels of N-palmitoyl-l-phenylalanine

“…For instance, Ding et al have shown no adverse effects for mixtures of N -AAA in mice diet at 5 wt%. 44 As proven by Perinelli, 45 N -lauroylserine or N -lauroylproline is not cytotoxic at concentrations as high as 1.5 wt%. These facts show the lack of innocuity of these compounds.…”

Sodium N-lauryl amino acids derived from silk protein can form catanionic aggregates with cytarabine as novel anti-tumor drug delivery systems

“…Sericin is needed to be solved in the treatment of silk industrial waste, and the preparation of sericin protein surfactant provides a new solution for silk industry waste. The properties and synthesis of SSS have been studied in detail by our team (Wu & Zhang, 2014;Ding et al, 2017). In this article, for the first time, we describe the formation of catanionic vesicles, formed using the anionic surfactant (SSS) and a cationic drug.…”