2013
DOI: 10.1128/jmbe.v14i1.531
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Biosafety Guidelines for Handling Microorganisms in the Teaching Laboratory: Development and Rationale

Abstract: The safe handling of microorganisms in the teaching laboratory is a top priority. However, in the absence of a standard set of biosafety guidelines tailored to the teaching laboratory, individual educators and institutions have been left to develop their own plans. This has resulted in a lack of consistency, and differing levels of biosafety practices across institutions. Influenced by the lack of clear guidelines and a recent outbreak of Salmonella infections that was traced back to te… Show more

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Cited by 70 publications
(73 citation statements)
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“…The RGs of all organisms used in our courses are verified on the database of the American Biological Safety Association each semester to ensure proper handling and biosafety precautions. At the beginning of each semester, our students are instructed in biosafety rules and regulations set forth by the American Society for Microbiology (ASM) Biosafety Guidelines (27) and the Rutgers Environmental Health and Safety (REHS) regulations for teaching laboratories (28). Our faculty also presents a short lecture about the importance of biosafety and reviews all the rules and procedures (Appendix 3), after which students sign an agreement to follow all safety precautions.…”
Section: Methodsmentioning
confidence: 99%
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“…The RGs of all organisms used in our courses are verified on the database of the American Biological Safety Association each semester to ensure proper handling and biosafety precautions. At the beginning of each semester, our students are instructed in biosafety rules and regulations set forth by the American Society for Microbiology (ASM) Biosafety Guidelines (27) and the Rutgers Environmental Health and Safety (REHS) regulations for teaching laboratories (28). Our faculty also presents a short lecture about the importance of biosafety and reviews all the rules and procedures (Appendix 3), after which students sign an agreement to follow all safety precautions.…”
Section: Methodsmentioning
confidence: 99%
“…These items are stored in the lab for the duration of the semester. American Society for Microbiology and REHS biosafety guidelines for BSL-1 are followed for hand hygiene (27, 28). Every student working in the lab uses an iPad to take notes and pictures, as well as keep an electronic notebook to minimize possible contamination (26).…”
Section: Methodsmentioning
confidence: 99%
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“…These organisms are classified as biosafety level 1 (BSL1) microbes (17). Instructors must refer to the American Society of Microbiology (ASM) Biosafety Guidelines for handling and working with these types of organisms in the teaching laboratory (2). The bacterial strains used during this project are environmental isolates from root nodules.…”
Section: Safety Issuesmentioning
confidence: 99%
“…The plates should be sealed, stored in a secure location, and only observed, not opened or subcultured. Subculturing of the samples should only be done in a BSL2 laboratory (2).…”
Section: Safety Issuesmentioning
confidence: 99%