Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system

Design of a microbial sensor using conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine

“…The biosensor showed a good linear range, and repeatability as well as a high operational stability. Besides, practical use of the present detection system for both ethanol and glucose analyses showed that it can properly be used in a chromatographic set-up as was shown previously with different enzyme electrodes such as pyranose oxidase [33], and sulfhydryl oxidase [34,35]. …”

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

“…The combination of GR and SOX allows the detection of GSSG and GSH in the same matrices. This biosensor has also been applied as an ECD after HPLC separation [133].…”

Silver nanoparticles/multiwalled carbon nanotube/polyaniline film for amperometric glutathione biosensor

“…A number of electrochemical biosensors, mostly based on oxidation of GSH on unmodified [22,23] or chemically modified electrodes [24,25], have been reported, of which amperometric biosensors were based on two gold electrodes and two complementary oligonucleotides [26], pyrrolofullerene bis-adduct [27], molecularly imprinted polymer [28], silicon nanowires [29], sulfhydryl oxidase bi-enzymatic system [30] and self assembled monolayers (SAMs) [31]. In most of these electrodes, the enzyme was immobilized onto support by electrostatic interaction or adsorption, which allows leakage of enzyme, resulting in a low stability of the enzyme electrode.…”