Biosensing bacterial 16S rDNA by microchip electrophoresis combined with a CRISPR system based on real-time crRNA/Cas12a formation

Luo, Feifei; Geng, Xing; Li, Zhi; Dai, Ge; Zhang, Chu; He, Pingang; Zhang, Fan; Wang, Qingjiang

doi:10.1039/d2ra03069a

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…[ 205 ] Recently, ME has been used for the detection of infectious agents namely bacteria, viruses, and parasites by analyzing their nucleic acids. For example, Luo et al [ 206 ] combined ME with the CRISPR‐Cas12a system to detect bacterial 16S rDNA. The proposed method involved real‐time crRNA generation and the crRNA/Cas12a post‐assembly mode, which could eliminate background signals.…”

Section: Detection Techniquesmentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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Section: Detection Techniquesmentioning

confidence: 99%