Biosensing strategies for the electrochemical detection of viruses and viral diseases – A review

Brazaca, Laís Canniatti; Santos, Pãmyla L. dos; Oliveira, Paulo Roberto de; Rocha, Diego P.; Stefano, Jéssica S.; Kalinke, Cristiane; Muñoz, Rodrigo A.A.; Bonacin, Juliano Alves; Janegitz, Bruno C.; Carrilho, Emanuel

doi:10.1016/j.aca.2021.338384

Cited by 90 publications

(46 citation statements)

References 270 publications

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“…The crisis with COVID-19 has highlighted the need for analytical devices to monitor and detect high-incident diseases caused by viruses and other pathogens with rapid propagation and mutations. Low-cost, portable analytical sensors [1][2][3] are perhaps the only alternative for the diagnosis of such infections as the one caused by the coronavirus (SARS-CoV-2) [4,5]. Indeed, the lack of such sensors explains why developing countries could not test their population to the extent recommended by the World Health Organization, which would have been essential for the monitoring to prevent the person-to-person transmission chain [6,7].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

et al. 2022

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This paper reports the development of a low-cost (< US$ 0.03 per device) immunosensor based on gold-modified screenprinted carbon electrodes (SPCEs). As a proof of concept, the immunosensor was tested for a fast and sensitive determination of S proteins from both SARS-CoV and SARS-CoV-2, by a single disposable device. Gold nanoparticles were electrochemically deposited via direct reduction of gold ions on the electrode using amperometry. Capture antibodies from spike (S) protein were covalently immobilized on carboxylic groups of self-assembled monolayers (SAM) of mercaptoacetic acid (MAA) attached to the gold nanoparticles. Label-free detection of S proteins from both SARS-CoV and SARS-CoV-2 was performed with electrochemical impedance spectroscopy (EIS). The immunosensor fabricated with 9 s gold deposition had a high performance in terms of selectivity, sensitivity, and low limit of detection (LOD) (3.16 pmol L −1 ), thus permitting the direct determination of the target proteins in spiked saliva samples. The complete analysis can be carried out within 35 min using a simple one-step assay protocol with small sample volumes (10 µL). With such features, the immunoplatform presented here can be deployed for mass testing in point-of-care settings.

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Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

et al. 2022

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“…In the past two years, researchers have developed numerous biosensors for the analysis and detection of the current still spreading SARS-CoV-2 virus pandemic. 43,44 The main concern is improving selectivity, sensitivity, speed, and throughput and enabling in situ detection. 45 In this work, we developed a rGO-based fluorescence assay for rapid, amplification-free and high-throughput SARS-CoV-2 RNA detection.…”

Section: Sensors and Diagnostics Papermentioning

confidence: 99%

Rapid, amplification-free and high-throughput SARS-CoV-2 RNA detectionviaa reduced-graphene-oxide based fluorescence assay

et al. 2022

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“…Mustapha Kamil 等 [58] 发展了一种光纤传感器, 用于登革热病毒的免疫复合物无标记检测 , 灵敏度和检测限分别为 5.02 nm/(nmol/L)和 1 pmol/L, 标准偏差不大于±0.4, 但这种传感器的敏感度受表面污染影响较大. 为此, Brazaca 等 [72] 用聚丙烯生物脱离方法将核酸捕获探针固定在平行 1D 光子晶体谐振器传感器表面, 可去除 98%的残基, 用多种模式检测登革热病毒. Huang 等 [73] 基于光子晶体表面的感测能力, 发展了可调谐的垂直腔表面发射激光器(VCSEL), 用于登革热 IgG 抗体的检测, 尽管该方法比 ELISA 方法灵敏度高, 但仍有假阳性.…”

Section: 光子晶体生物传感器unclassified

Biosensing Technology for Dengue Virus Detection

Shi¹,

Xia²,

He³

et al. 2022

Acta Chimica Sinica

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Dengue virus is a single-stranded RNA virus transmitted by mosquitoes, belonging to the flaviviridae. Currently, there are no targeted antiviral drugs and effective virus vaccines of timely prevention. Due to the importance of clinical detection, new methods for detecting Dengue virus infection have emerged in recent years, trying to detect and control this infection early. This review mainly describes the biosensing technologies for the detection of Dengue virus related diagnostic markers, including optical, electrochemical, enzyme-linked immunosorbent assay and other biosensing methods, and comprehensively evaluates the sensitivity, accuracy, concentration range, detection limit and clinical application ability of different detection methods, so as to further improve the detection performance of these technologies, and to promote the development of this field. It will provide references for the early diagnosis of Dengue virus infection.

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Biosensing strategies for the electrochemical detection of viruses and viral diseases – A review

Cited by 90 publications

References 270 publications

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

Rapid, amplification-free and high-throughput SARS-CoV-2 RNA detectionviaa reduced-graphene-oxide based fluorescence assay

Biosensing Technology for Dengue Virus Detection

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