Biosensing using laser-polarized xenon NMR/MRI

Berthault, Patrick; Huber, Gaspard; Desvaux, Hervé

doi:10.1016/j.pnmrs.2008.11.003

Cited by 112 publications

(89 citation statements)

References 126 publications

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“…The typical Hyper-CEST agent has a hydrophobic xenon binding site in a cage-like molecule that can be functionalized to bind molecular targets. Prominent examples for imaging applications are based on cryptophane-A (CrA) [10][11][12] or cucurbit [6,7]uril (CB6,7). [13][14][15] (Hyper-)CEST efficiency scales with the concentration of bound nuclei, [B] (Figure 1).…”

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confidence: 99%

Protein Nanostructures Produce Self-Adjusting Hyperpolarized Magnetic Resonance Imaging Contrast through Physical Gas Partitioning

Kunth

Witte

et al. 2018

ACS Nano

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Signal amplification strategies are critical for overcoming the intrinsically poor sensitivity of nuclear magnetic resonance (NMR) reporters in noninvasive molecular detection. A mechanism widely used for signal enhancement is chemical exchange saturation transfer (CEST) of nuclei between a dilute sensing pool and an abundant detection pool. However, the dependence of CEST amplification on the relative size of these spin pools confounds quantitative molecular detection with a larger detection pool typically making saturation transfer less efficient. Here we show that a recently discovered class of genetically encoded nanoscale reporters for 129 Xe magnetic resonance overcomes this fundamental limitation through an elastic binding capacity for NMR-active nuclei. This approach pairs high signal amplification from hyperpolarized spins with ideal, self-adjusting saturation transfer behavior as the overall spin ensemble changes in size. These reporters are based on gas vesicles, i.e., microbe-derived, gas-filled protein nanostructures. We show that the xenon fraction that partitions into gas vesicles follows the ideal gas law, allowing the signal transfer under hyperpolarized xenon chemical exchange saturation transfer (Hyper-CEST) imaging to scale linearly with the total xenon ensemble. This conceptually distinct elastic response allows the production of quantitative signal contrast that is robust to variability in the concentration of xenon, enabling virtually unlimited improvement in absolute contrast with increased xenon delivery, and establishing a unique principle of operation for contrast agent development in emerging biochemical and in vivo applications of hyperpolarized NMR and magnetic resonance imaging.

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confidence: 99%

Protein Nanostructures Produce Self-Adjusting Hyperpolarized Magnetic Resonance Imaging Contrast through Physical Gas Partitioning

Kunth

Witte

et al. 2018

ACS Nano

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“…Xenon encapsulated into cryptophane derivative cages can be detected and exploited by NMR spectroscopy. The great sensitivity of xenon to local environment combined with the use of hyperpolarization techniques led to an important variation of the NMR chemical shifts [231][232][233]. Additional signal amplification can be obtained by chemical exchange saturation transfer (hyperCEST) [234,235].…”

Section: Supramolecular Compoundsmentioning

confidence: 99%

129Xenon NMR: Review of recent insights into porous materials

Weiland

Springuel‐Huet

Nossov

et al. 2016

Microporous and Mesoporous Materials

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“…SPIO contrast agents have been essentially used clinically for diagnosis of liver diseases, [83] whereas USPIO probes are generally used for lymph-node imaging, angiography, and bloodpool imaging [84][85][86][87][88][89]. Besides their clinical use, MRI contrast agents based on iron oxide nanoparticles are actually developed for studying biological processes: Significant contributions in this research area have illustrated the potential use of these particles for molecular and cellular imaging applications [78,80,82,[90][91][92][93][94].…”

Section: T 2 Nps-based Contrast Agentsmentioning

confidence: 99%

Functionalized nanomaterials: their use as contrast agents in bioimaging: mono- and multimodal approaches

Trequesser¹,

Seznec²,

Delville³

2016

Nano Online

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The successful development of nanomaterials illustrates the considerable interest in the development of new molecular probes for medical diagnosis and imaging. Substantial progress was made in synthesis protocol and characterization of these materials whereas toxicological issues are sometimes incomplete. Nanoparticle-based contrast agents tend to become efficient tools for enhancing medical diagnostics and surgery for a wide range of 2 imaging modalities. Multimodal nanoparticles (NPs) are much more efficient than conventional molecular-scale contrast agents. They provide new abilities for in vivo detection and enhanced targeting efficiencies through longer circulation times, designed clearance pathways, and multiple binding capacities. Properly protected, they can safely be used for the fabrication of various functional systems with targeting properties, reduced toxicity and proper removal from the body. This review mainly describes the advances in the development of mono-to multimodal NPs and their in vitro and in vivo relevant biomedical applications ranging from imaging and tracking to cancer treatment. Besides specific applications for classical imaging, (MRI, PET, CT, US, PAI) are also mentioned less common imaging techniques such as terahertz molecular imaging (THMI) or ion beam analysis (IBA).Perspectives on multimodal theranostic NPs and their potential for clinical advances are also mentioned.

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Biosensing using laser-polarized xenon NMR/MRI

Cited by 112 publications

References 126 publications

Protein Nanostructures Produce Self-Adjusting Hyperpolarized Magnetic Resonance Imaging Contrast through Physical Gas Partitioning

Protein Nanostructures Produce Self-Adjusting Hyperpolarized Magnetic Resonance Imaging Contrast through Physical Gas Partitioning

129Xenon NMR: Review of recent insights into porous materials

Functionalized nanomaterials: their use as contrast agents in bioimaging: mono- and multimodal approaches

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