Biosensing with DNAzymes

Abstract

This article provides a comprehensive review of biosensing with DNAzymes, providing an overview of different sensing applications while highlighting major progress and seminal contributions to the field of portable biosensor devices and point-of-care diagnostics.

A localized DNA finite-state machine with temporal resolution

“…Although the inputs are designed as hairpin structures, we have demonstrated that transducer components could be introduced, allowing transformation of common biological signals such as miRs into the standard hairpin inputs. Besides miR inputs, the FSM has the potential to incorporate functional moieties on nucleic acids, such as aptamers ( 23 , 49 , 51 ), DNAzymes ( 52 ), therapeutic oligos ( 53 ), reactive chemicals ( 54 ), metal nanoparticles ( 22 ), and proteins ( 24 – 26 , 36 ) to achieve various formats of molecular inputs and outputs. For example, temporal information of biological molecules involved in diseases could be used to guide drug releases or program functional molecular devices.…”

Carbon Dots in Bioimaging, Biosensing and Therapeutics: A Comprehensive Review

“…Biosensing is a sub‐field of analytical sensing, which places special attention on incorporating molecular biometric entities in the detection process. [ 151–153 ] The excellent photophysical properties of CDs such as photoluminescence (PL) and good electrical conductivity make them valuable biosensing materials. [ 154–156 ] Generally, the sensing principle of CDs is based on the specific interaction between the surface functional groups or targeting moiety and the analytes, leading to the change of CDs’ optical emission characteristics ( Figure a).…”

“…Biosensing is a sub-field of analytical sensing, which places special attention on incorporating molecular biometric entities in the detection process. [151][152][153] The excellent photophysical properties of CDs such as photoluminescence (PL) and good electrical conductivity make them valuable biosensing materials. [154][155][156] Generally, the sensing principle of CDs is based on the specific interaction between the surface functional groups or targeting moiety and the analytes, leading to the change of CDs' optical CTAC and triethanolamine MSN Provide drug loading sites Cancer cell targeting and drug delivery [276] Pyrene and polyethylenimine Black phosphorus Enhanced NIR absorption Photothermal-chemo combination therapy [267] Citric acid and urea ZIF Carrier for ECL labels and enhancing the ECL signals Biosensor [302] Pyrene and polyethylenimine MXene Marrow-bandgap nanosonosensitizer Sonodynamic therapy [303] Di(Dopa-Se) Hydrogels Self-healing and adhesion properties in vivo cancer detection [304] Figure 4.…”

DNA-based enzymatic systems and their applications

“…Dzs-based sensors can work as a promising candidate for ion detection in vitro , including Mg 2+ , Ni + , Na + , Zr 2+ , K + , and so on ( Wu et al., 2017 ; Liu et al., 2021b ; Chen et al., 2017 ; McConnell et al., 2021 ; Cheng et al., 2020 , 2021 ; Ren et al., 2020 ). Liu’s group developed a lanthanide-specific Dzs array for the separation of lanthanides from non-lanthanides ( Huang et al., 2016 ).…”