Biosensing with polydiacetylene materials: structures, optical properties and applications

Abstract

Polydiacetylene (PDA) materials are used as a platform for detection of biological analytes such as microorganisms, viruses and proteins. The environmentally responsive chromic and emissive properties of the polymer, combined with self-assembled material formats, make these materials particularly attractive for biosensing applications. A variety of approaches have been used in developing these materials and demonstrating their potential for biological detection. In this feature article we describe different PDA material formats, discuss the optical properties that are the basis for signal generation, and review the use of PDA for biosensing.

Preparation of optical active polydiacetylene through gelating and the control of supramolecular chirality

“…Recently, the supramolecular control on the conformation of polymers by non-covalent bond proved to be an alternative way to obtain the optically active polymers [4][5][6][7]. Polydiacetylene (PDA), as one of the most investigated polymers, exhibited many potential applications as biosensors, pathogenic agents, and functional materials [8][9][10][11]. It is well known that diacetylene undergoes topochemical reactions upon UV-irradiation by 1,4-addition reactions when the monomer units are aligned appropriately [12,13].…”

Colorimetric response of azobenzene-terminated polydiacetylene vesicles under thermal and photic stimuli

“…Polydiacetylene is a class of conjugated polymers derived from a 1,4-addition polymerization of the corresponding diacetylene monomer. Polydiacetylene exhibit intense chromatic switch from blue to red in response to external stimuli and these colorimetric changes can be easily perceived by UV-absorption spectrum, fluorescence or even the naked eyes [2]. Various polydiacetylene have been developed as colorimetric sensing materials for chemical and biological systems.…”

Effect of phospholipid insertion on arrayed polydiacetylene biosensors

“…The colorimetric change causes vesicles to turn fluorescent [3,14,15]. Micro-patterned PDA vesicles can be fluorescence-based sensor chips for external stimulation [16][17][18][19], or as a biochip for high-throughput detection of low-volume analytes [20].…”