Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries

Abstract

The developed biosensor assay can measure release of substances from vascular preparations. Histamine is released from the vessel preparation in response to aldosterone treatment independently of mast cells. The assay enables us to study a new signaling mechanism for vascular responses induced by aldosterone.

New insights into the astonishing diversity of hormone functions

“…The mineralocorticoid aldosterone is secreted by adrenocortical glomerulus cells. 5 It was reported that aldosterone mediates the release of histamine from perivascular mast cells. Under conditions of salt deficiency and depletion of fluids, the aldosterone production is stimulated by the RAS.…”

“…Aldosterone controls arterial blood pressure by controlling salt and water balance. 5 The novel biosensor successfully measures the release of substances from vascular preparations. The cleavage of angiotensinogen results in a higher activity of the protease renin, which causes a higher plasma concentration of angiotensin II.…”

“…Under conditions of salt deficiency and depletion of fluids, the aldosterone production is stimulated by the RAS. 5 Angiotensin II, another hormone that is part of the RAS and various ROS are also potent modulators of vascular function. This is a hormone that primarily stimulates the production of aldosterone in the glomerular cells.…”

“…The effects of aldosterone are induced by activated genomic mineralocorticoid receptor-mediated pathways but also via nongenomic effects and mineralocorticoid receptor-independent effects. 5 It was reported that aldosterone mediates the release of histamine from perivascular mast cells. A biosensor for realtime detection of histamine release after stimulation with aldosterone was developed in order to prove this hypothesis and to directly measure histamine release.…”

“…A biosensor for realtime detection of histamine release after stimulation with aldosterone was developed in order to prove this hypothesis and to directly measure histamine release. 5 The novel biosensor successfully measures the release of substances from vascular preparations. For aldosterone, a new signalling pathway that causes histamine release was identified, as the detected aldosterone-induced histamine release from mesenteric blood vessels was independent of perivascular mast cells.…”