Biosensor technology for determination of BOD

Current research activity in biosensors

“…The development of BOD sensors as biosensor technology has also been summarized in a review article [204]. BOD is currently considered to be the most important parameter of wastewater quality.…”

Development of Biological Oxygen Demand Biosensor for Monitoring the Fermentation Industry Effluent

“…Biochemical oxygen demand (BOD) is an important index for monitoring organic pollutants in water. The conventional standard method (five day BOD test, BOD 5 ), however, is a complicated and a time-consuming procedure, including a five-day incubation, and also requires considerable experience and skill to get reproducible results [ 4 ]. Fast determination of BOD could be achieved by biosensor-based methods.…”

Research progress on the rapid, real time, on line, <italic>in situ</italic> monitoring water quality biochemical oxygen demand

“…年发明的微生物传感器方法最为经典 [2] . 具体是将在 实验室内预先培养好的已知种类的微生物菌种通过固 定化材料包埋成膜, 覆盖在DO传感探头表面构成微生 物传感器, 其结构示意图及基本工作原理如图1所示 [3] . [8] .…”

“…为了改善这些缺陷, Jiang等 [9] 以钌复合物构建光学氧敏感膜并包埋从海水 中分离纯化的三株菌种用于高盐海水的BOD快速测 定, 以期望实现所固定菌种对环境污染物较好的适应 力; Tan等 [10] 采用预先热处理方式杀死包埋的复合菌 种, 一定程度上避免了微生物膜保存过程中活性降低 及使用过程中菌种的增殖等问题; Jia等 [11] [13] . 然而, 电活性细菌厌氧代谢有机物产生 的电子需渗透过电活性菌膜达到阳极, 与阴极构成闭 [3] (网 络版彩图) Figure 1 Component of microbial biosensor (a) and its principle (b) in BOD determination. In Figure (b), A presents the steady state of the biosensor in buffer, B presents the signal decrease process by adding organic substrate, C presents the steady state after adding organic substrate in buffer, D presents the biosensor recovery process after washing, and S presents the adding of organic substrate [3] [14] .…”

“…然而, 电活性细菌厌氧代谢有机物产生 的电子需渗透过电活性菌膜达到阳极, 与阴极构成闭 [3] (网 络版彩图) Figure 1 Component of microbial biosensor (a) and its principle (b) in BOD determination. In Figure (b), A presents the steady state of the biosensor in buffer, B presents the signal decrease process by adding organic substrate, C presents the steady state after adding organic substrate in buffer, D presents the biosensor recovery process after washing, and S presents the adding of organic substrate [3] [14] . 更为重要的是, 微生物燃料电池参 与反应的产电微生物均为厌氧微生物, 而水中某些耗 氧污染物的代谢分解是必须依靠耗氧微生物完成的, 如硝化细菌氧化还原态氨的耗氧过程, 因此, 采用微生 物燃料电池检测BOD在原理上存在一定的技术缺 陷 [15] .…”