The use of nanomaterials in biomedical applications has revolutionized drug delivery, biosensing, and tissue engineering. These materials offer improved bioavailability, targeted delivery, and controlled release, enhancing therapeutic efficacy and reducing side effects. They are also used in cancer therapy, gene delivery, and vaccination. Nanomaterial-based biosensors detect biomolecules at ultra-low concentrations, enhancing disease diagnosis and monitoring. In tissue engineering, nanomaterials create scaffolds mimicking the extracellular matrix, promoting cell adhesion, proliferation, and differentiation. Techniques like electrospinning and 3D printing are used to create bioactive scaffolds for regenerative medicine. This chapter highlights the transformative potential of nanomaterials in biomedical applications, addressing challenges and future directions in this rapidly evolving field.