Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

Jaffrézic‐Renault, Nicole; Martelet, C.; Chevolot, Yann; Cloarec, Jean-Pierre

doi:10.3390/s7040589

Cited by 117 publications

(62 citation statements)

References 46 publications

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“…The second method consists on the use of the polysaccharidic region located in the Fc region of the antibody and the use of aminated nanoparticles. To our knowledge, few contributions in this line can be found on literature with magnetic microbeads and still less with magnetic nanoparticles [25][26][27][28][29][30][31][32]. The biofunctionalization of magnetic nanoparticles suffers some limitations when compared to surfaces and microbeads, such as easy aggregation with small changes of pH and/or ionic strength, polymeric shell instability, batch to batch MNPs irreproducibility, or presence of surfactants.…”

Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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There is a growing interest in the use of magnetic nanoparticles (MNPs) for their application in quantitative and highly-sensitive biosensors. The use of them as labels of biological recognition events and their detection by means of some magnetic method constitutes a very promising strategy for quantitative high-sensitive lateral-flow assays.In the present article, we report the importance of nanoparticle functionalization for the improvement of sensitivity for a lateral flow immunoassay. More precisely, we have found that immobilization of IgG anti-hCG through its polysaccharide moieties on magnetic nanoparticles allows more successful recognition of the hCG hormone. Although we used the detection of hCG as a model in this work, the strategy of binding antibodies to MNPs through its sugar chains reported here is applicable to other antibodies. Its potential is huge as it will be very useful for the development of quantitative and high-sensitive lateral-flow assays for its use on human and veterinary, medicine, food and beverage manufacturing, pharmaceutical, medical biologics and personal care product production, environmental remediation, etc.

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Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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show abstract

“…Typically, a magnet is used to hold beads with a magnetic core steady against a flow or the magnet is moved around to promote mixing. 4 The advantage of microbead based assays over conventional plate arrays is the high surface area of the bead which promotes higher reaction rates, the flexibility of being able to choose which beads are injected, and lower cost. However, the magnetic based systems do not offer fine control over bead position.…”

Section: Introductionmentioning

confidence: 99%

Flexible Acoustic Particle Manipulation Device with Integrated Optical Waveguide for Enhanced Microbead Assays

et al. 2009

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Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads into an optical evanescent field generated by a laser pumped ion-exchanged waveguide. The evanescent field only excites fluorophores brought within ~100 nm of the waveguide, allowing the system to differentiate between targets bound to the beads and those unbound and still held in suspension. The radiation forces are generated in a standing-wave chamber that supports multiple acoustic modes, permitting particles to be both attracted to the waveguide surface and also repelled. To provide further control over particle position, a novel method of switching rapidly between different acoustic modes is demonstrated, through which particles are manipulated into an arbitrary position within the chamber. A novel type of assay is presented: a mixture of streptavidin coated and control beads are driven towards a biotin functionalised surface, then a repulsive force is applied, making it possible to determine which beads became bound to the surface. It is shown that the quarter-wave mode can enhance bead to surface interaction, overcoming potential barriers caused by surface charges. It is demonstrated that by measuring the time of flight of a microsphere across the device the bead size can be determined, providing a means of multiplexing the detection, potentially detecting a range of different target molecules, or varying bead mass.

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“…These assays may be realised using magnetic beads held in devices using magnetic forces [1], however acoustic radiation forces can be used to manipulate a wider range of beads, including magnetic and non-magnetic beads, as they rely on acoustic properties only. The approach also allows finer control over bead position.…”

Section: Introductionmentioning

confidence: 99%

Multi-modal particle manipulator to enhance bead-based bioassays

et al. 2010

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By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalized glass surface and polystyrene microbeads, and identify those that bind to the surface by illuminating bound beads using an evanescent field generated by guided light. School of Electronics and Computer Science, University of Southampton, SO17 1BJ, UKThe movement towards and immobilization of streptavidin coated beads onto a biotin functionalized waveguide surface is achieved by using a quarter-wavelength mode pushing beads onto the surface, while the removal of non-specifically bound beads uses a second quarter-wavelength mode which exhibits a kinetic energy maximum at the boundary between the carrier layer and fluid, drawing beads towards this surface. This has been achieved using a multi-modal acoustic device which exhibits both of these quarter-wavelength resonances. Both 1-D acoustic modelling and finite element analysis has been used to design this device and to investigate the spatial uniformity of the field.We demonstrate experimentally that 90% of specifically bound beads remain attached after applying ultrasound, with 80% of non-specifically bound control beads being successfully removed acoustically. This approach overcomes problems associated with lengthy sedimentation processes used for bead-based bioassays and surface (electrostatic) forces, which delay or prevent immobilisation. We explain the potential of this technique in the development of DNA and protein assays in terms of detection speed and multiplexing.PACS code: 43.90 v

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Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

Cited by 117 publications

References 46 publications

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Flexible Acoustic Particle Manipulation Device with Integrated Optical Waveguide for Enhanced Microbead Assays

Multi-modal particle manipulator to enhance bead-based bioassays

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