Biosensors and bioelectronics on smartphone for portable biochemical detection

Zhang, Diming; Liu, Qingjun

doi:10.1016/j.bios.2015.08.037

Cited by 562 publications

(233 citation statements)

References 93 publications

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“…A comparison of lab- and smartphone-based methods is given in ESM Table S4. The most popular optical approach to smartphone detectors is based on colorimetric reactions such as in LFIA or ELISA [28]. Colorimetric smartphone-based sensing conventionally relies on the phone’s complementary metal oxide semiconductor (CMOS) filters to assign red, green, blue (RGB) values to light.…”

Section: Methods For Food Allergen Detection Using a Smartphone Readomentioning

confidence: 99%

“…For an in-depth review into all existing smartphone-based diagnostic devices, Quesada-Gonzalez and Merkoçi can be referred to [27]. For a more focused review concerning biosensors and bioelectronics on a smartphone see Zhang and Liu [28]. General approaches to smartphone-based food diagnostics have been recently reviewed by Rateni et al [29] and Choi [12], which addressed the necessity and market-gap for user-friendly food detection.…”

Section: Introductionmentioning

confidence: 99%

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Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

2018

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In this critical review, we provide a comprehensive overview of immunochemical food allergen assays and detectors in the context of their user-friendliness, through their connection to smartphones. Smartphone-based analysis is centered around citizen science, putting analysis into the hands of the consumer. Food allergies represent a significant worldwide health concern and consumers should be able to analyze their foods, whenever and wherever they are, for allergen presence. Owing to the need for a scientific background, traditional laboratory-based detection methods are generally unsuitable for the consumer. Therefore, it is important to develop simple, safe, and rapid assays that can be linked with smartphones as detectors to improve user accessibility. Smartphones make excellent detection systems because of their cameras, embedded flash functions, portability, connectivity, and affordability. Therefore, this review has summarized traditional laboratory-based methods for food allergen detection such as enzyme-linked-immunosorbent assay, flow cytometry, and surface plasmon resonance, and the potential to modernize these methods by interfacing them with a smartphone readout system, based on the aforementioned smartphone characteristics. This is the first review focusing on smartphone-based food-allergen detection methods designed with the intention of being consumer-friendly. Graphical abstractA smartphone-based food allergen detection system in three easy steps (1) sample preparation, (2) allergen detection on a smartphone using antibodies, which then transmits the data wirelessly, (3) analytical results sent straight to smartphone Electronic supplementary materialThe online version of this article (10.1007/s00216-018-0989-7) contains supplementary material, which is available to authorized users.

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Section: Methods For Food Allergen Detection Using a Smartphone Readomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

2018

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“…In addition, this device shows computational capability and operation interface better than typical cellphones. 24 Other crucial advantage is the high number of subscriptions worldwide. Currently, approximately 1.9 billion people are smartphone's users.…”

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confidence: 99%

Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

Camargo¹,

Vicentini²,

Gobbi³

et al. 2016

J. Braz. Chem. Soc.

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We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%. Keywords: rapid test, albumin, human plasma, colorimetry, negatoscope IntroductionBradford assay is one of the most usual methods applied for determining protein in solution. [1][2][3][4] This method was proposed by Marion M. Bradford in 1976 5 and relies on the interaction of Comassie Brilliant Blue G-250 dye with the protein. After a short-time incubation, the anionic product of such reaction is optically monitored at 595 nm. 6 The advantages of the Bradford assay are that its routine is user-friendly, fast, reproducible, sensitive and selective to the target molecule. 7,8 Conversely, some pharmaceutical excipients interfere on the response of this approach even at low contents. In this case, the precipitation of the protein with sucrose showed to be an efficient alternative.9 Other bottleneck concerns the poor portability and relatively high cost (ca. US$ 10,000.00) of the spectrophotometer applied in the Bradford assays. These features undermine the compatibility of the method toward point-of-care (POC) analyses. 10POC platforms present low cost and high portability, robustness and simplicity. Accordingly, such technologies bypass the necessity for specialized personal and allow in situ measurements even at resource-limited environments, presenting key social and economic implications. Such methods have been recently used at diverse applications. [11][12][13][14][15][16] The POC technology is the major segment of the global in vitro diagnostics market. 11Nonetheless, despite the advantages of these methods, only a few commercial executions had been observed because of the production cost of such analytical platforms. Such drawback inhibits the creation of profitable businesses. 17We address herein a POC platform for performing the Bradford assay using smartphone-based optical detection. The smartphones have been widely used to the development of POC analytical tools for different colorimetric quantitative analyses, including immunoassays, biochemical and healthcare experiments. [18][19][20][21][22] These applications are possible because the smartphones present the two functional components necessary for accomplishing optical detection: source (light-emitting diode, LED, white light from flash) and radiation detector (digital camera). 23 This camera exhibits resolution and sensitivity greater than those of conventional phone camera...

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“…Portability and even wearability [217] of biosensors are other important recent developments [218]. The amazing potential of the modern smartphone has led to a plethora of innovative developments in this area [219]. Finally, it is hoped that MNPs could break through all barriers in bioanalyses, having almost infinite potentials and tremendous scope of functionalities.…”

Section: Discussionmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

303

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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Biosensors and bioelectronics on smartphone for portable biochemical detection

Cited by 562 publications

References 93 publications

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

Consumer-friendly food allergen detection: moving towards smartphone-based immunoassays

Smartphone for Point-of-Care Quantification of Protein by Bradford Assay

Noble metal nanoparticles in biosensors: recent studies and applications

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