Biosensors and multiple mycotoxin analysis

Gaag, Bram van der; Spath, Sabine; Dietrich, Heidelinde R. C.; Stigter, E.C.A.; Boonzaaijer, G.; Osenbruggen, Ton van; Koopal, Kees

doi:10.1016/s0956-7135(03)00008-2

Cited by 172 publications

(81 citation statements)

References 4 publications

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“…The mycotoxins were then extracted using chloroform and filtration, and the produced mycotoxins were concentrated. Finally, the mycotoxins were classified and characterized by mycotoxins analysis using TLC (6)(7)(8). This experiment resulted in the production and the identification of 18 different mycotoxins from one or more species of Penicillium.…”

Section: Types Of Mycotoxins and Their Natural Commoditiesmentioning

confidence: 99%

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

et al. 2017

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Abstract. The chemical nature of most of the mycotoxins makes them highly liposoluble compounds that can be absorbed from the site of exposure such as from the gastrointestinal and respiratory tract to the blood stream where it can be dissimilated throughout the body and reach different organs such as the liver and kidneys. Mycotoxins have a strong tendency and ability to penetrate the human and animal cells and reach the cellular genome where it causes a major mutagenic change in the nucleotide sequence which leads to strong and permanent defects in the genome. This defect will eventually be transcribed, translated and lead to the development of cancer. In this review, the chemical and physical nature of mycotoxins, the action of mycotoxins on the cellular genome and its effect on humans, mycotoxins and their carcinogenicity and mycotoxins research gaps are discussed, and new research areas are suggested. The research review posed various questions. What are the different mycotoxins that can cause cancer, what is the role of mycotoxins in causing cancer and what types of cancers can be caused by mycotoxins? These questions have been selected due to the significant increase in the mycotoxin contamination and the cancer incidence rate in the contemporary world. By revealing and understanding the role of mycotoxins in developing cancer, measures to reduce the risks and incidents of cancer could be taken.

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Section: Types Of Mycotoxins and Their Natural Commoditiesmentioning

confidence: 99%

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

et al. 2017

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“…Electrochemical immunosensors with surface-adsorbed antibodies on screen-printed carbon electrodes have been fabricated for the detection of aflatoxin M1 in milk (Micheli et al 2005) and, in an array configuration, for the detection of aflatoxin B1 (Pemberton et al 2006). Affinity-based surface plasmon resonance sensors (SPR) have the advantage of not requiring any labelling of the target mycotoxin (Tü dö s et al 2003) and may become an alternative method for rapid screening, which also enables the simultaneous detection of multiple mycotoxins using serial connected flow cells (van der Gaag et al 2003). In a further label-free immunochemical approach for the detection of aflatoxin B1 and ochratoxin A, optical waveguide lightmode spectroscopy (OWLS) was used with integrated optical waveguide sensor chips measuring the resonance incoupling angle of polarized light, thus determining the surface coverage (Adányi et al 2007).…”

Section: Immunochemical Techniquesmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

312

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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“…It is planned to detect at least nine different mycotoxins in parallel (3). Another assay is designed as an inhibition assay (52), in which a fixed concentration of mycotoxin specific antibody is mixed with a sample containing an unknown amount of mycotoxin, whereby antibody and mycotoxin form a complex. The sample is then passed over a sensor surface to which mycotoxin has been immobilized.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The application of PCR in the detection of mycotoxigenic fungi in foods

Konietzny¹,

Greiner²

2003

Braz. J. Microbiol.

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It is estimated that 25 to 50% of the crops harvested worldwide are contaminated with mycotoxins. Because of the toxic and carcinogenic potential of mycotoxins, there is an urgent need to develop detection methods that are rapid and highly specific. The highly advanced physico-chemical methods for the analysis of mycotoxins in use, have the disadvantage that highly sophisticated clean-up and/or derivatization procedures must be applied. An alternative could be the detection of the mycotoxigenic moulds themselves, especially as molecular techniques have been introduced recently as powerful tools for detecting and identifying fungi. PCR methods for the detection of aflatoxigenic Aspergilli, patulin-producing Penicillum and trichothecene-as well as fumonisin-producing Fusaria strains have been described. The usefulness of the PCR methods developed so far to monitor quality and safety in the food an feed industry was already demonstrated. Thus, PCR may be applied to the screening of agricultural commodities for the absence of mycotoxin producers prior to or even after processing. Negative results in this assay indicate that a sample should be virtually free of mycotoxins. Only the positive samples left must be analyzed for the presence of mycotoxins using physicochemical standard methods. This review does not only summarize the so far developed qualitative and quantitative PCR assays for the detection of mycotoxigenic fungi in agricultural commodities, foods and animal feeds, but describes also strategies to develop new specific PCR assays for such a detection.

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Biosensors and multiple mycotoxin analysis

Cited by 172 publications

References 4 publications

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

Effects of different mycotoxins on humans, cell genome and their involvement in cancer

Mycotoxin analysis: An update

The application of PCR in the detection of mycotoxigenic fungi in foods

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