Biosensors for cardiac biomarkers detection: A review

Qureshi, Anjum; Gürbüz, Yaşar; Niazi, Javed H.

doi:10.1016/j.snb.2012.05.077

Cited by 342 publications

(211 citation statements)

References 93 publications

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“…The major impetus in the research and development of such virus sensors is their practical features such as the ease of operation, simple and straightforward device fabrication, possibility to integrate synthetic or natural antibodies, rapid response, high selectivity and cross-sensitivity, portability, and miniaturization capability. It is due to these advantages that biosensors have not only been widely employed for virus recognition [14], but have also been applied for the detection of microorganism such as pathogenic bacteria [15,16] and yeast [17][18][19][20], living blood cells [21][22][23], and different diseases biomarkers [24,25].…”

Section: Introductionmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Introductionmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…cTnI is only restricted inside the myocardium and it is currently considered to be the standard biomarker test for detecting AMI, because it is significantly more specific than other cardiac biomarkers [18]. The levels of cTnI in healthy humans are normally lower than 0.1 ng mL −1 , and a level between 0.1and 2 ng mL −1 indicates a diagnosis of unstable angina and other heart disorder, whereas levels greater than 2 ng mL −1 indicate an increased risk for future serious heart events [19]. This emphasizes the need for developing diagnostic tools which are highly sensitive, cost effective, and utilizing existing large-scale manufacturing techniques.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay for troponin I using a glassy carbon electrode modified with a hybrid film consisting of graphene and multiwalled carbon nanotubes and decorated with platinum nanoparticles

et al. 2016

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This article describes a bioelectrode for the determination of human cardiac troponin-I (cTnI). A glassy carbon electrode was coated with a hybrid film of graphene and multiwalled carbon nanotube (G-MWCNT) and modified with platinum nanoparticles (Pt NPs) that were capped with mercaptopropionic acid. The PtNPs were anchored on the G-M W C N T h y b r i d f i l m v i a t h e c r o s s -l i n k e r 1 -pyrenemethylamine and subsequently functionalized with antibody against troponin (anti-cTnI). The bioelectrode was characterized by transmission electron microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The performance of the immunoelectrode was investigated by electrochemical impedance spectroscopy, and response was fit to Randle's equivalent circuit model. The charge transfer resistance (R et ) at a.c. frequencies of <1 Hz is found to be a viable sensing parameter. The dissociation constant of the immunoreaction between surface immobilized anti-cTnI and the analyte cTnI is 0.29 nM (with a Hill coefficient of 0.23), this indicating a negative cooperativity and high binding affinity of cTnI for anti-cTnI on the electrode surface. The EIS response is linear in the 1.0 pg mL −1 to 10 ng mL −1 concentration range, and the R et sensitivity is 145.5 Ω cm 2 per decade.

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“…These are the largest single cause of death in the EU, accounting 42% deaths in 2010 [1]. Following cardiovascular biomarkers is a key tool in the fight against cardiovascular diseases, aimed at preserving the patient's life and delivering effective therapies and a successful prognosis for the disease [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…× × × × × (Bio)Sensors could be an alternative, because they allow rapid diagnosis in point-of-care, providing reduced response times and leading to better outcomes of the disease, most especially in acute events [2,14]. The receptor element is at the core of the device selectivity, an essential condition for detecting proteins that circulate in the blood at quite low concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…The receptor element is at the core of the device selectivity, an essential condition for detecting proteins that circulate in the blood at quite low concentrations. Natural receptors namely antibodies, nucleic acids, and enzymes, have been widely used in biosensors for targeting cardiac biomarkers, leading to electrochemical, optical, mass or magnetic changes [2]. In general, natural receptors offer high selectivity towards a target analyte, but show decreased chemical stability, and are rather expensive.…”

Section: Introductionmentioning

confidence: 99%

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Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

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This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon-sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing prot ein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate-rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The d evices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 µg/mL, respectively, with detection limits of 1.5 and 0.8 µg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivit y and selectivity.

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Biosensors for cardiac biomarkers detection: A review

Cited by 342 publications

References 93 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Immunoassay for troponin I using a glassy carbon electrode modified with a hybrid film consisting of graphene and multiwalled carbon nanotubes and decorated with platinum nanoparticles

Protein-responsive polymers for point-of-care detection of cardiac biomarker

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