Biosensors for determination of total antioxidant capacity of phytotherapeutic integrators: comparison with other spectrophotometric, fluorimetric and voltammetric methods

Campanella, Luigí; Bonanni, A.; Bellantoni, D.; Tomassetti, M.

doi:10.1016/s0731-7085(03)00627-7

Cited by 24 publications

(32 citation statements)

References 22 publications

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“…In previous papers [32,33] results obtained by means of SOD biosensor for measurement of antioxidant power of some phototherapeutic integrators, were compared with other methods cited in literature like cyclic voltammetry [7], spectrophotometry [34] and fluorimetry [35]. A spectrophotometric method was chosen for this comparison, namely the N,N-dimethylp-phenylenediamine (DMPD-FeCl 3 ) method [7] as well as the fluorimetric Oxygen Radical Absorbance Capacity (ORAC) method [35].…”

Section: Discussionmentioning

confidence: 99%

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Multiparametric probe as screening test for quality and safety evaluation in foods

Campanella

Gatta

Pintore

2009

Sens. & Instrumen. Food Qual.

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A probe for a screening evaluation test of quality and safety of foods based on four different amperometric biosensors all having Clark's electrode as transducer and different biological systems as components is described. Specifically three amperometric biosensors (tyrosinase, cyclooxygenase and superoxide dismutase biosensor) and a respirometric test are the components of the probe. For each proposed sensor robustness and LODs were evaluated. The latter ones for each analyte are compared with Maximum Residue Limits (MRLs) admitted in foods according to EEC Norm. Lastly, two different simple extraction procedures of the analytes to be detected from the food matrix have been planned and set up, in any case obtaining more than 70% extracted.

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Section: Discussionmentioning

confidence: 99%

“…The repeatability of biosensor method is quite good (RSD% = 5-10%) compared to that one of ORAC method (10%) and DMPD-FeCl 3 method (13%). The voltammetric method has shown a good repeatability (RSD% \ 1%), however its sensitivity is not uniform for all types of samples [33].…”

Section: Discussionmentioning

confidence: 99%

Multiparametric probe as screening test for quality and safety evaluation in foods

Campanella

Gatta

Pintore

2009

Sens. & Instrumen. Food Qual.

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“…Antioxidant enzymes (AOE) include superoxide dismutase, catalase, and glutathione peroxidase and indirectly glutathione reductase (8,9). Antioxidants can generally neutralize free radicals by giving an electron or at least inhibit the chain reactions that lead to tissue damage (10). Evaluation of plasma total antioxidant capacity is important for the diagnosis of diseases.…”

Section: Introductionmentioning

confidence: 99%

Simple and Sensitive Method of Fluorometry for Determination of Total Antioxidant Capacity

2018

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Background and Objective:Oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. Total antioxidant capacity plays a significant role in the body's antioxidant defense, so its assessment is a matter of the utmost importance. Currently, assessment of this capacity is performed in various scientific fields by expensive imported kits. The aim of the present study was to design a sensitive fluorometric method for the assessment of total antioxidant capacity and improvement of sensitivity, accuracy, and speed of the measurement.Methods: The sensitivity and intra-and inter-assay accuracy, verification by recovery and parallelism tests, method comparison, and correlation and coherence evaluation were performed. To increase the accuracy and speed of reading, the assay was performed in a microplate and reading was done using a fluorometer plate.Result: In accuracy assessment, intra-and inter-assay coefficient of variation was calculated to be 4.1-5.7 and 4.4-7.5, respectively. In validity evaluation, the recovery percentage was calculated to be 90-109, the recovery percentage range was 90 to 109. Comparison of the results of this method on 50 serum samples with common colorimetric method, indicated a good correlation (0.93). The sensitivity of the studied method was 0.01 mM/l.Conclusion: Microplate-reader fluorometry, in addition to increasing the speed of measurement, has enough efficacy, accuracy, and sensitivity to assess total antioxidant capacity and could be an appropriate alternative for current colorimetric methods.

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“…Various methods for characterisation of total antioxidant potential are presently in routine use, although some are non-stoichiometric (Alimelli et al, 2007;Campanella et al, 2004;Careri et al, 2003;Carralero Sanz et al, 2005;De Beer et al, 2005;Fernandez-Pachon et al, 2004;Giovanelli, 2005;Gomez-Alonso et al, 2007;Magalhaes et al, 2009;Makris et al, 2003;Malovana et al, 2001;Mozetič et al, 2006;Prior et al, 2005;Prosen et al, 2007;Recamales et al, 2006;Spigno  De Faveri, 2007;Staško et al, 2008;Weingerl et al, 2009;Woraratphoka, 2007). Some of these methods allow for rapid characterization of wines, and allow for evaluation of synergistic effects of various wine components, e.g.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Analytical Methods for Determination of Polyphenols in Wine by HPLC/UV-Vis, Spectrophotometry and Chemiluminometry

Weingerl¹

2012

Macro to Nano Spectroscopy

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Biosensors for determination of total antioxidant capacity of phytotherapeutic integrators: comparison with other spectrophotometric, fluorimetric and voltammetric methods

Cited by 24 publications

References 22 publications

Multiparametric probe as screening test for quality and safety evaluation in foods

Multiparametric probe as screening test for quality and safety evaluation in foods

Simple and Sensitive Method of Fluorometry for Determination of Total Antioxidant Capacity

A Comparative Study of Analytical Methods for Determination of Polyphenols in Wine by HPLC/UV-Vis, Spectrophotometry and Chemiluminometry

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