Biosensors for pathogen detection: A smart approach towards clinical diagnosis

Singh, Renu; Mukherjee, Maumita Das; Sumana, Gajjala; Gupta, Rajinder K.; Sood, Seema; Malhotra, Bansi D.

doi:10.1016/j.snb.2014.03.005

Cited by 158 publications

(73 citation statements)

References 112 publications

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“…There have been numerous reviews describing current and potential applications of biosensing devices and the preferred features of such a device [1][2][3][4][5][6]. Pathogen detection and identification is one such application [7]. This area is important in surveillance and health monitoring and also for diagnostics to facilitate appropriate treatment choices.…”

Section: Introductionmentioning

confidence: 99%

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Nguyen

Hill

Warren‐Smith

et al. 2015

Sensors and Actuators B: Chemical

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In all cases accepted manuscripts should:  link to the formal publication via its DOI  bear a CC-BY-NC-ND license -this is easy to do, click here to find out how  if aggregated with other manuscripts, for example in a repository or other site, be shared in alignment with our hosting policy  not be added to or enhanced in any way to appear more like, or to substitute for, the published journal article Abstract: This work presents a novel biosensor using the multimode interference effect in an exposed core microstructured optical fiber (ECF). In this work biotin molecules are immobilized onto the ECF core surface to serve as the capturing probe for streptavidin, the target molecules.Since each distinct guided mode in the ECF interacts with the surrounding medium differently, the interference between any two specific modes will experience a fringe shift (or phase change) upon a change in the refractive index (RI) of the surrounding medium, or a localized RI change on the surface of the ECF core as a result of a biological binding event. In our experiment, the interferometric sensing platform was realized by splicing a section of ECF with lead-in and leadout single mode fibers (SMFs). An interference pattern is obtained in the transmission spectrum as the result of multiple excited modes (excited and re-collected at the lead-in and lead-out splicing points) propagating in the ECF with different propagation constants. The interference pattern is non-uniform, indicating that there are more than two modes involved. Fast Fourier transform (FFT) is used to separate individual interference patterns that contribute to this complex spectrum and monitor their phase changes upon RI variation of the surrounding medium. In this way multiple RI sensitivities can be realized because each spatial frequency possesses a distinct sensitivity with respect to the surrounding RI. The operation of this device was validated by measuring the phase changes that occur when the sensing platform was subjected to solutions of different RIs or functionalized with different molecules. A biosensor was demonstrated based on this novel platform using biotin as the capturing probe to detect streptavidin with low non-specific adsorption. The proposed platform is reliable, cost-effective, and offers a potential label-free biosensing alternative to the widely used surface plasmon resonance (SPR) technique.

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Section: Introductionmentioning

confidence: 99%

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Nguyen

Hill

Warren‐Smith

et al. 2015

Sensors and Actuators B: Chemical

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“…Jarocka et al 151 described an immunosensor for the avian influenza hemagglutinin (HA) H5, where gold electrode was modified by gold colloidal nanoparticles functionalized by antibody-binding fragments of anti-H5 monoclonal antibodies. The antigenantibody interaction was explored with EIS in the presence of [Fe(CN) 6 ] 3−/4− . 151 The immunosensor was able to recognize three different His-tagged variants of recombinant HA (H5N1) with detection limit of 2.2 pg mL −1 .…”

Section: Eis Immunobiosensormentioning

confidence: 99%

“…A way of signal conversion depends on the type of physicochemical change resulting from the initial and final signal. 6 The most frequently used biological component of sensors are enzymes, [7][8][9][10] antibodies, 8,11 and oligonucleotides. [12][13][14] Therefore, the biosensor differs from the sensor by the presence of biological (biorecognition) component, which usually exhibits a bioaffinity or biocatalytic role.…”

Section: Introductionmentioning

confidence: 99%

“…117 Chen et al 118 described EB for detection of HIV based on a cascade hybridization of the capture probe, target, and two auxiliary probes and formation of micrometerlong one-dimensional DNA nanostructures. To target nanostructure formation and signal amplification, redox indicator [Ru(NH 3 ) 6 ] 3+ was used. 118 Immunoelectrochemical biosensors and protein affinity EB Electrochemical immunosensors could potentially replace routinely used ELISA for diagnosis of viral diseases.…”

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confidence: 99%

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Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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“…In contrast, conventional diagnostic methods often utilize time-consuming techniques such as microscopy and cultivation in different media [3] and bear the risk of false-positive surfaces, for example, to assess cleaning success in a hospital environment, which is receiving increasing interest [10]. This project was performed and has successfully competed in the International Genetically Engineered Machine (iGEM) competition 2014 [11].…”

Section: Introductionmentioning

confidence: 99%

Development of a Modular Biosensor System for Rapid Pathogen Detection

Hanke¹,

Bailly²,

Demling³

et al. 2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Progress in the field of pathogen detection relies on at least one of the following three qualities: selectivity, speed, and cost-effectiveness. Here, we demonstrate a proof of concept for an optical biosensing system for the detection of the opportunistic human pathogen Pseudomonas aeruginosa while addressing the abovementioned traits through a modular design. The biosensor detects pathogen-specific quorum sensing molecules and generates a fluorescence signal via an intracellular amplifier. Using a tailored measurement device built from low-cost components, the image analysis software detected the presence of P. aeruginosa in 42 min of incubation. Due to its modular design, individual components can be optimized or modified to specifically detect a variety of different pathogens. This biosensor system represents a successful integration of synthetic biology with software and hardware engineering.

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Biosensors for pathogen detection: A smart approach towards clinical diagnosis

Cited by 158 publications

References 112 publications

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Nanoscale virus biosensors: state of the art

Development of a Modular Biosensor System for Rapid Pathogen Detection

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