Biosensors for the Detection of Bacterial and Viral Clinical Pathogens

Henríquez, Luis Castillo; Brenes-Acuña, Mariana; Castro-Rojas, Arianna; Cordero-Salmerón, Rolando; Lopretti-Correa, Mary; Vega-Baudrit, José

doi:10.3390/s20236926

Cited by 113 publications

(56 citation statements)

References 236 publications

(223 reference statements)

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“…GQD probes show excellent biocompatibility, and they easily penetrate the cell membrane and enter the cell owing to their small size. They constitute single or few layers of graphene particles that exhibit tunable surface functionality and long-term resistance to photobleaching ( Castillo-Henriquez et al, 2020 ; Alavi et al, 2021 ). GQDs based on an “off-on” FRET mechanism have been widely used in the detection of various microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

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Functional microorganisms play a vital role in removing environmental pollutants because of their diverse metabolic capability. Herein, a function-oriented fluorescence resonance energy transfer (FRET)-based graphene quantum dots (GQDs-M) probe was developed for the specific identification and accurate sorting of azo-degrading functional bacteria in the original location of environmental samples for large-scale culturing. First, nitrogen-doped GQDs (GQDs-N) were synthesized using a bottom-up strategy. Then, a GQDs-M probe was synthesized based on bonding FRET-based GQDs-N to an azo dye, methyl red, and the quenched fluorescence was recovered upon cleavage of the azo bond. Bioimaging confirmed the specific recognition capability of GQDs-M upon incubation with the target bacteria or environmental samples. It is suggested that the estimation of environmental functional microbial populations based on bioimaging will be a new method for rapid preliminary assessment of environmental pollution levels. In combination with a visual single-cell sorter, the target bacteria in the environmental samples could be intuitively screened at the single-cell level in 17 bacterial strains, including the positive control Shewanella decolorationis S12, and were isolated from environmental samples. All of these showed an azo degradation function, indicating the high accuracy of the single-cell sorting strategy using the GQDs-M. Furthermore, among the bacteria isolated, two strains of Bacillus pacificus and Bacillus wiedmannii showed double and triple degradation efficiency for methyl red compared to the positive control (strain S12). This strategy will have good application prospects for finding new species or high-activity species of specific functional bacteria.

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Section: Introductionmentioning

confidence: 99%

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

et al. 2021

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“…An optimum composition for high-antimicrobial activity against S. aureus , compared with E. coli bacteria was patented. For possible use as a fast absorbent carrier of anti-COVID-19 drug delivery, some researchers are trying to prepare scaffolding that would be able to carry such drugs while controlling the degree of a drug’s absorption by the human body—something that would be indispensable [ 240 , 241 ]. However, this new applied nanotechnology is not yet mastered for use with newly discovered drugs.…”

Section: Electrospun Nanofiber Applications For Medical Care In Thmentioning

confidence: 99%

Electrospun Nanofibrous Scaffolds: Review of Current Progress in the Properties and Manufacturing Process, and Possible Applications for COVID-19

et al. 2021

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Over the last twenty years, researchers have focused on the potential applications of electrospinning, especially its scalability and versatility. Specifically, electrospun nanofiber scaffolds are considered an emergent technology and a promising approach that can be applied to biosensing, drug delivery, soft and hard tissue repair and regeneration, and wound healing. Several parameters control the functional scaffolds, such as fiber geometrical characteristics and alignment, architecture, etc. As it is based on nanotechnology, the concept of this approach has shown a strong evolution in terms of the forms of the materials used (aerogels, microspheres, etc.), the incorporated microorganisms used to treat diseases (cells, proteins, nuclei acids, etc.), and the manufacturing process in relation to the control of adhesion, proliferation, and differentiation of the mimetic nanofibers. However, several difficulties are still considered as huge challenges for scientists to overcome in relation to scaffolds design and properties (hydrophilicity, biodegradability, and biocompatibility) but also in relation to transferring biological nanofibers products into practical industrial use by way of a highly efficient bio-solution. In this article, the authors review current progress in the materials and processes used by the electrospinning technique to develop novel fibrous scaffolds with suitable design and that more closely mimic structure. A specific interest will be given to the use of this approach as an emergent technology for the treatment of bacteria and viruses such as COVID-19.

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“…The development of a promising theranostic agent, which is safe and natural medicine with diagnostic capability, might be advantageous [ 14 , 15 , 16 ]. Over the last decade, different biosensing platforms accompanied by functional materials have been developed for various infectious and non-infectious diseases [ 9 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. Among different functional materials, carbon dots (CDs) have gained enormous scientific attention in view of their unique physiochemical properties.…”

Section: Introductionmentioning

confidence: 99%

Allium sativum derived carbon dots as a potential theranostic agent to combat the COVID-19 crisis

Kalkal

Allawadhi

Pradhan

et al. 2021

Sensors International

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Coronavirus disease 2019 (COVID-19) is one of the worst pandemics to have hit humanity. The manifestations are quite varied, ranging from severe lung infections to being asymptomatic. Hence, there is an urgent need to champion new tools to accelerate the end of this pandemic. Compromised immunity is a primary feature of COVID-19. Allium sativum (AS) is an effective dietary supplement known for its immune-modulatory, antibacterial, anti-inflammatory, anticancer, antifungal, and anti-viral properties. In this paper, it is hypothesized that carbon dots derived from AS (AS-CDs) may possess the potential to downgrade the expression of pro-inflammatory cytokines and revert the immunological aberrations to normal in the case of COVID-19. Carbon dots (CDs) have already been explored in the world of nanobiomedicine as promising theranostic candidates for bioimaging and drug/gene delivery. The antifibrotic and antioxidant effects of AS are elaborated, as demonstrated in several studies. It is found that the most active constituent of AS, allicin has a highly potent antioxidant and reactive oxygen species (ROS) scavenging effect. The antibacterial, antifungal, and anti-viral effects along with their capability of negating inflammatory effects and cytokine storm, are discussed. The synthesis of theranostic CDs from AS may provide a novel weapon in the therapeutic armamentarium for the management of COVID-19 infection and, at the same time, could act as a diagnostic agent for COVID-19.

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Biosensors for the Detection of Bacterial and Viral Clinical Pathogens

Cited by 113 publications

References 236 publications

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Function-Oriented Graphene Quantum Dots Probe for Single Cell in situ Sorting of Active Microorganisms in Environmental Samples

Electrospun Nanofibrous Scaffolds: Review of Current Progress in the Properties and Manufacturing Process, and Possible Applications for COVID-19

Allium sativum derived carbon dots as a potential theranostic agent to combat the COVID-19 crisis

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