Biosensors : the stabilité problem

Gibson, Tim

doi:10.1051/analusis:1999270630

Cited by 66 publications

(34 citation statements)

References 36 publications

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“…The prototype tests in real samples have critical stages such as immobilization of the biocomponent during the construction of the device and preparation of the sample for analysis. Biosensors require mild temperature and pH conditions to keep the biological element active (Gibson, 1999;Wilson and Gifford, 2005). Consequently, in some cases a previous treatment of the sample is recommended to eliminate interfering species such as ascorbic acid, tyrosine and others.…”

Section: Enzymatic Biosensorsmentioning

confidence: 99%

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Benzi

Stefanazzi

Ferrero

2009

Chilean J. Agric. Res.

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Rice weevil (Sitophilus oryzae L.) is a primary insect pest of stored grain. The development of resistance resulted in the application of synthetic insecticides. In recent years many plant essential oils have provided potential alternatives to currently used insect control agents. The Brazilian pepper tree (Schinus molle L. var. areira (L.) DC.) (Anacardiaceae) ) (Anacardiaceae) has different biological properties such as insecticidal activity. In this study, repellent, fumigant activity, nutritional indices, and feeding deterrent action were evaluated on S. oryzae adults. Filter paper impregnation was used to test fumigant toxicity, whereas treated whole wheat was used to evaluate repellent activity and a flour disk bioassay was done to evaluate feeding deterrent action and nutritional index alteration. Leaf essential oils showed repellent effects at both concentrations (0.04 and 0.4% w/w), while fruit essential oils lacked repellent activity. Both plant oils altered nutritional indices. Fruit essential oils had a strong feeding deterrent action (62%) while leaves had a slight effect (40.6%). With respect to fumigant activity, neither of the essential oils was found to be toxic. Resistance and toxicity problems of the synthetic insecticides have resulted in the necessity of finding more effective and healthier alternatives. Thus, essential oils are the most tested products presently (Papachristos and Stamopoulus, 2002; Umoetok and Gerard, 2003; Zhang et al., 2004; Tapondjou et al., 2005; Ferrero et al., 2006; Sánchez Chopa et al., 2006, Stefanazzi et al., 2006, Wang et al., 2006. Different biological activities of plant derivatives have been demonstrated for the control of stored grain pests (Golob et al., 1999; Rajendran and Sriranjini, 2008).The Brazilian pepper tree (Schinus molle L. var. areira (L.) DC.) is associated with agricultural crops in boundaries, windbreaks, riverbank protection, and watershed conservation. In popular medicine, it is use for its astringent, diuretic, antispasmodic properties among others. In pest control, it is used as a fumigant, repellent, and ovicide (Ruffínengo et al., 2005; Ferrero et al., 2006).The objective of the present study was to evaluate the fumigant, repellent and/or attractant, feeding deterrent activity, and the alterations in the nutritional physiology produced by the essential oils from leaves and fruits of S. molle var. areira on 3-to 4-day-old S. oryzae adults. V. BENZI et al. -BIOLOGICAL ACTIVITY OF ESSENTIAL OILS… MATERIALS AND METHODS InsectsThe insects came from a susceptible colony maintained in the Cátedra de Zoología Agrícola de la Universidad Nacional de Buenos Aires (UBA). They were bred in glass containers, 8 cm in diameter, 13 cm high, sealed with fine netting, maintained at 28 ± 1 ºC, 60-70% HR, a 12:12 photoperiod, and using whole wheat grains as food. Vegetal materialThe vegetal organs (leaves and mature fruits) of the pepper tree were collected during the summer period in Bahía Blanca city, Buenos Aires, Argentina (38°41' S; 62°17' ...

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Section: Enzymatic Biosensorsmentioning

confidence: 99%

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Benzi

Stefanazzi

Ferrero

2009

Chilean J. Agric. Res.

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“…Thus, a net dglucose sensor was produced combining mutarotase and glucose oxidase. One of the most important problems often encountered in the development of commercial biosensors is the stabilization of the enzymes in the bioactive layer [127]. Jawaheer proposed the use of a pectin matrix for enzyme immobilization, because it can easily be reconstituted into a thin gel.…”

Section: Enzymatic Biosensor Arraysmentioning

confidence: 99%

Electroanalysis Utilizing Amperometric Microdisk Electrode Arrays

et al. 2007

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This article reviews the literature dealing with electroanalytical applications of ultramicroelectrode arrays over the past twenty years. A brief theoretical description of the mass transport mode governing their behavior is given, after which the main fabrication methods are described. The applications described in the later sections of this review range from conventional electroanalysis, namely trace metal stripping methods, using mercury modified arrays to more recent advances in the field of biosensors (enzymatic, immunosensors and nucleic acid based ones).

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“…As a theoretical upper limit for the total process time of enzymatic processes, the stabilities achieved with biosensors are considered where enzymes have been shown to be active for two years. [29] Together with the data for the ideal electrochemical process, the theoretical operational window can be drawn as shown in Figure 2. These calculated performance parameters are of course only theoretical maxima to define the absolute framework for electroenzymatic processes.…”

Section: Operational Windows Of Electroenzymatic Processesmentioning

confidence: 99%

Productivity of Selective Electroenzymatic Reduction and Oxidation Reactions: Theoretical and Practical Considerations

Ruinatscha¹,

Höllrigl²,

Otto³

et al. 2006

Adv Synth Catal

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The volumetric productivities, final product concentration and total process times, of electroenzymatic processes comprising reductive electrochemical cofactor regeneration coupled to dehydrogenase or oxygenase catalyzed redox reactions determine process performance. Exemplified for the production of fine chemicals, operational windows were defined to consider these parameters. This theoretical approach allows the identification of limiting process parameters and promising process developments. Several biocatalytic processes for syntheses of specialty chemicals are already in a performance range of technical relevance. Electroenzymology thus evolved to a strong additional technology in the toolbox of biocatalysis and organic chemistry.

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Biosensors : the stabilité problem

Cited by 66 publications

References 36 publications

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Electroanalysis Utilizing Amperometric Microdisk Electrode Arrays

Productivity of Selective Electroenzymatic Reduction and Oxidation Reactions: Theoretical and Practical Considerations

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