Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Ma, Xiaohua; Hao, Yuanqiang; Dong, Xiaoxiao; Xia, Ning

doi:10.3390/molecules28114394

Cited by 9 publications

(3 citation statements)

References 165 publications

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“…Nanomaterials with a large surface area and plenty of functional groups can serve as nanocarriers to load numerous enzymes for signal amplification [ 144 , 145 , 146 , 147 , 148 ]. Qu et al used ALP-modified silica nanoparticles (SiO 2 NPs) and ALP-encapsulating liposomes as the signal labels to trigger enzymatic silver metallization for the detection of prostate-specific antigen (PSA) [ 149 , 150 ].…”

Section: Electrochemical Methodsmentioning

confidence: 99%

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Chen,

La,

et al. 2023

Biosensors

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Electrochemical immunosensors have shown great potential in clinical diagnosis, food safety, environmental protection, and other fields. The feasible and innovative combination of enzyme catalysis and other signal-amplified elements has yielded exciting progress in the development of electrochemical immunosensors. Alkaline phosphatase (ALP) is one of the most popularly used enzyme reporters in bioassays. It has been widely utilized to design electrochemical immunosensors owing to its significant advantages (e.g., high catalytic activity, high turnover number, and excellent substrate specificity). In this work, we summarized the achievements of electrochemical immunosensors with ALP as the signal reporter. We mainly focused on detection principles and signal amplification strategies and briefly discussed the challenges regarding how to further improve the performance of ALP-based immunoassays.

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Section: Electrochemical Methodsmentioning

confidence: 99%

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Chen,

La,

et al. 2023

Biosensors

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“…[7][8][9][10] Furthermore, the abnormal concentrations of these bio-signals often correlate with specific diseases in organisms. 11,12 Monitoring their levels enables a more accurate and efficient estimation of diseases, significantly reducing the time required for clinical diagnosis. 13 Beyond diagnostic benefits, continuous tracking of these bio-signals facilitates more convenient and precise treatment of chronic diseases.…”

Section: Introductionmentioning

confidence: 99%

Highly-ordered assembled organic fluorescent materials for high-resolution bio-sensing: a review

Wang,

Chen,

Zhang

et al. 2024

Biomater. Sci.

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“…For instance, natural enzymes serving as biocatalytic labels in immunoassays have been widely integrated with nanomaterials for signal amplification, such as horseradish peroxidase (HRP), alkaline phosphatase (ALP), and glucose oxidase (GOx). Although elevating the number of enzyme labels in one immunoreaction event can promote the catalysis reaction and enhance the detection sensitivity, the immobilization of enzymes and antibodies on the solid surface may limit the enzymatic activity and the molecular recognition ability [5,6]. The general immobilization strategies, such as direct physical adsorption and covalent coupling, may cause the random orientation and conformational change of proteins, partially or completely suppressing the catalytic activity of enzymes and the binding ability of antibody and target.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

Sun,

Yi,

Liu

et al. 2024

Molecules

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The sensitivity of immunoassays is generally limited by the low signal reporter/recognition element ratio. Nanomaterials serving as the carriers can enhance the loading number of signal reporters, thus improving the detection sensitivity. However, the general immobilization strategies, including direct physical adsorption and covalent coupling, may cause the random orientation and conformational change in proteins, partially or completely suppressing the enzymatic activity and the molecular recognition ability. In this work, we proposed a strategy to load recognition elements of antibodies and enzyme labels using boronic acid-modified metal-organic frameworks (MOFs) as the nanocarriers for signal amplification. The conjugation strategy was proposed based on the boronate ester interactions between the carbohydrate moieties in antibodies and enzymes and the boronic acid moieties on MOFs. Both enzymes and MOFs could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2, therefore achieving dual signal amplification. To indicate the feasibility and sensitivity of the strategy, colorimetric immunoassays of prostate specific antigen (PSA) were performed with boronic acid-modified Cu-MOFs as peroxidase mimics to catalyze TMB oxidation and nanocarriers to load antibody and enzyme (horseradish peroxidase, HRP). According to the change in the absorbance intensity of the oxidized TMB (oxTMB), PSA at the concentration range of 1~250 pg/mL could be readily determined. In addition, this work presented a site-specific and oriented conjugation strategy for the modification of nanolabels with recognition elements and signal reporters, which should be valuable for the design of novel biosensors with high sensitivity and selectivity.

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Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Cited by 9 publications

References 165 publications

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Highly-ordered assembled organic fluorescent materials for high-resolution bio-sensing: a review

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

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