Bioseparation

Abstract

The article contains sections titled: 1. Introduction 2. Cell Disruption 3. Solid–Liquid Separation 3.1. Conventional Filtration 3.2. Membrane Filtration 3.2.1. Promotion of Turbulence 3.2.2. Application of Electric Field 3.3. Centrifugation 3.4. Coagulation and Flocculation 4. Product Isolation 4.1. Extraction 4.2. Adsorption Including Ion Exchange 4.2.1. Affinity Adsorption 4.2.2. Ion‐Exchange Adsorption 4.2.3. Quantitative Analysis 4.2.4. Elution 4.3. Precipitation 4.3.1. Salting Out 4.3.2. pH Adjustment 4.3.3. Organic Solvents 4.3.4. Temperature 4.3.5. Polymers and Polyelectrolytes 4.3.6. Affinity Precipitation 4.4. Solubilization and Refolding 4.5. Ultrafiltration 5. Purification 5.1. Chromatography 5.1.1. Gel Filtration Chromatography 5.1.2. Affinity Chromatography 5.1.3. Reverse Phase and Hydrophobic Interaction Chromatography 5.1.4. Quantitative Aspects 5.2. Electrical Separations 5.2.1. Electrophoresis 5.2.2. Isoelectric Focusing and Isotachophoresis 5.2.3. Electrodialysis 5.2.4. Dielectrophoresis 5.3. Magnetic Separations 5.4. Membrane Chromatography 6. Polishing 6.1. Crystallization 6.2. Drying and Freeze Drying 6.3. Buffer Exchange 6.4. Virus and Endotoxin Removal 6.5. Removal of Aggregates 7. New Bioseparation Strategies

Amino-Acid Adsorption in MFI-Type Zeolites Enabled by the pH-Dependent Ability to Displace Water

“…Amino acids are building blocks of life and are used in biotechnology and industry with a world market larger than 4 million tons per year and an annual growth of 5–8% . For example, the food industry uses phenylalanine for the production of synthetic sweeteners and glutamic acid for the production of flavor enhancers. , Other amino acids are used for cosmetic and pharmaceutical purposes. , Peptides and proteins consist of amino acids and play an important role in different application fields such as medicine and nutrition. − Products such as amino acids, peptides, and proteins are commonly produced biochemically. − However, downstream processing of biochemical streams can be very difficult due to the compound complexity and is often the most costly part of a biochemical process . Consequently, the development of efficient downstream processing is essential to reduce costs in biochemical production .…”

Production Rate‐Dependent Key Performance Indicators for a Systematic Design of Biochemical Downstream Processes

“…Main challenges typically arise from the fragile nature of the components handled and the complexity of the materials processed. Addressing these challenges by trial and error results in cost-intensive downstream processes that may account for up to 80 % of the total production costs [2].…”