BioSMB™ Technology: Continuous Countercurrent Chromatography Enabling a Fully Disposable Process

Development and Testing of a 4-Columns Periodic Counter-Current Chromatography System Based on Membrane Adsorbers

“…This method allowed an improvement in capacity utilization and continuous sample introduction. In the 1950s, various methods for continuous chromatography were developed, mainly to purify metals or wastewater by liquid-liquid extraction [12]. These (chromatography) methods were based on the counter-current principle: the mobile and stationary phases move in opposite directions.…”

“…Recent developments have made membrane chromatography particularly interesting for the purification of biomolecules [20]. (Disposable) membrane adsorbers (MA) [12] can be used in continuous low-pressure chromatography systems. The ligands are attached to the membrane surface so that the mass transport is mainly due to convection and not to pore diffusion [21].…”

Integrated system for temperature-controlled fast protein liquid chromatography. II. Optimized adsorbents and ‘single column continuous operation’

“…The switch from batch to continuous operation promises several advantages, key of which is more efficient utilization of the bed [43]. Several continuous chromatography formats have been developed and applied for the separation of biomacromolecules thus far, including Continuous Annular Chromatography [44], Continuous Radial Flow Chromatography [43], Simulated Moving Bed in various guises [45][46][47][48][49], and Periodic Counter-current Chromatography [50]. The new addition described here, Continuous Travelling Cooling Zone Reactor Chromatography, employs a single column operated isocratically.…”

Buffer recycling in downstream processing of biologics

“…The process is designed such that the protein A elution fraction goes directly to the intermediate purification. Also Tarpon has proposed integrated purification processes [53 ]. These processes save buffer by integration of unit operations but they do not gain further benefit from buffer recycling.…”