Biosolubilization of Polish brown coal by Gordonia alkanivorans S7 and Bacillus mycoides NS1020

Romanowska, Irena; Strzelecki, Bartosz; Bielecki, Stanisław

doi:10.1016/j.fuproc.2014.12.019

Cited by 54 publications

(24 citation statements)

References 22 publications

(36 reference statements)

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“…Bacillus sp. Y7 isolated from weathered lignite minerals in China was shown to solubilize Chinese lignite (Jiang et al, 2013), and Bacillus mycoides NS1020 degraded the coal from one of the Polish brown coal mines (Romanowska et al, 2015). In both cases more than one third of the lignite was degraded in about two weeks.…”

Section: Microbial Communitymentioning

confidence: 99%

Lignite biodegradation under conditions of acidic molasses fermentation

Detman

Bucha

Simoneit

et al. 2018

International Journal of Coal Geology

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Lignite is difficult to degrade, thus stimulation of the autochthonous lignite microflora and introduction of additional microorganisms are required for lignite decomposition. Here, a packed bed reactor, filled with lignite samples from the Konin region (central Poland) was supplied continuously with M9 medium, supplemented with molasses (a by-product from the sugar industry), for 124 days to stimulate the autochthonous lignite microflora. Acidic fermentation of molasses was observed in the bioreactor. The simultaneous decomposition of lignite occurred under this acidic molasses fermentation condition. Our results show decay of free (non-bound) organic compounds during anaerobic lignite biodegradation. The concentrations of n-alkanes, n-alkanols, n-alkanoic acids, diterpenoids, triterpenoids and steroids present in non-biodegraded samples decreased significantly (some compounds to zero) during biodegradation. Interestingly, other compound classes like phenols, ketones and certain organic compounds increased. We interpret this phenomenon as a gradual decomposition of polymers, lignin and cellulose, present in the lignite. These changes resulted from microbial activity since they were not observed in pure solutions of short-chain fatty acids. The 16SrRNA profiling of the microbial community selected in the bioreactor revealed that the dominant bacteria belonged to the Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes, furthermore representatives of 16 other phyla were also found. All the known taxa of lignocellulolytic bacteria were represented in the microbial community. Synergistic relations between bacteria fermenting molasses and bacteria degrading lignite are assumed. The results confirm lignin degradation in acidic medium by bacteria under anaerobic conditions.

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Section: Microbial Communitymentioning

confidence: 99%

Lignite biodegradation under conditions of acidic molasses fermentation

Detman

Bucha

Simoneit

et al. 2018

International Journal of Coal Geology

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“…The brown coal used in the study was obtained from Bełchatów Brown Coal Mine (Poland). Coal particles with a diameter of 1-2 mm were subjected to pretreatment with ozonation and then subjected to microbial solubilization as explained before 5,39 .…”

Section: Methodsmentioning

confidence: 99%

“…Brown coal modification: The experiment studying the impact of laccase on brown coal modification was done with concentrated culture supernatants of Gr2, 4C1 and 4A9 laccases. Firstly, brown coal pretreated by ozonation 39 was solubilized by Gordonia alkanivorans S7 5 . 200 µl of coal was mixed with 100 µl 100 mM DMP, 300 µl McIlvaine buffer pH 5.…”

Section: Methodsmentioning

confidence: 99%

Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae

Kwiatos

Jędrzejczak-Krzepkowska

Krzemińska

et al. 2020

Sci Rep

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Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three times lower Km, four times higher kcat and ten times higher catalytic efficiency than the parental enzyme. The strategy for laccase engineering was composed of a combination of random methods with a rational approach based on QM/MM MD studies of the enzyme complex with 2,6-dimethoxyphenol. Laccase mediator system with 2,6-dimethoxyphenol caused fulvic acids release from biosolubilized coal. Laccases are oxidoreductases that catalyze the 4-electron reduction of O 2 to water with simultaneous oxidation of organic substrates. Laccases are able to catalyze oxidation of a substrate of interest directly or indirectly by the formation of a radical, which then may take part in a non-enzymatic event that effects in the oxidation of a substrate of interest. Laccases oxidize phenolic substrates but are also able to oxidize non-phenolic or bigger substrates by Laccase Mediator System (LMS), where a small phenolic compound acts as a mediator 1. Laccases contain 4 copper atoms buried in their 3D structure, which are located in two separate Cu centers. T1 copper ion is a mononuclear center, whereas T2 copper ion and two T3 copper ions are positioned in T2/T3 copper center. The substrate is oxidized closed to T1 copper ion and then the electrons are transferred to the tri-nuclear center where O 2 is reduced to water. The coppers are coordinated by nearby located residues: the T1 copper ion by one Cys and two His residues, the T2 copper by two His residues and a solvent molecule and T3 copper to three His residues 2,3. Biosolubilization of brown coal is a clean coal technology that aims at the conversion of the lignite to its cleaner form or to change its structure to gain new features 4,5. Such solubilized material could be used as a source of value-added products 4. The liquid form of coal-microorganisms, such as Fusarium oxysporum reported before as an excellent coal solubilizer 6 , uses their metabolites, alkaline substances, biosurfactants and enzymes to turn the solid polymer to black liquid. Laccases, from bacteria and fungi, for example from species Pleurotus 7 or Streptomyces 8 , are known to take part in the degradation of lignin and lignite. These two polymers are similar in structure, thus, the mechanism of their degradation is expected to be the same. Lignin comprises only 10-20% of phenolic subunits. In theory, laccase could act on those subunits present on the lignin surface and modify the polymer. However, the possibility of lignin subunits entering the laccase active site is very limited. Moreover, it is not known to what extent lignin could be modified in this way 9. According to literature data, the treatme...

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“…In 1989, Quigley reported that an alkaline metabolite produced by fungi could ionize the acidic functional groups of low-ranked coal, thus improving the hydrophilicity of coal [23]. In the experiment of lignite biodegradation with crude oil hydrocarbon-degrading bacteria, the extracellular solubilization activities of tested bacteria decreased with the decrease of pH, indicating the effect of some alkaline chemicals on the process of coal liquefaction [24]. Moreover, it was proved that alkali treatment could break, disrupt, and reform various interaction forces and entanglements between coal macromolecules [25].…”

Section: Introductionmentioning

confidence: 99%

The effect of NaOH pretreatment on coal structure and biomethane production

Guo

Zhang

et al. 2020

PLoS ONE

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Biogenic CBM is an important component of detected CBM, which is formed by coal biodegradation and can be regenerated by anaerobic microorganisms. One of the rate-limiting factors for microbial degradation is the bioavailability of coal molecules, especially for anthracite which is more condense and has higher aromaticity compared with low-rank coal. In this paper, NaOH solution with different concentrations and treating time was employed to pretreat anthracite from Qinshui Basin to alter the coal structure and facilitate the biodegradation. The results showed that the optimal pretreatment conditions were 1.5 M NaOH treating for 12 h, under which the biomethane production was increased by 17.65% compared with untreated coal. The results of FTIR and XRD showed that NaOH pretreatment mainly reduced the multi-substituted aromatics, increased the CO in alcohols and aromatic ethers and the branching degree of aliphatic chain, and decreased the aromatic ring structure, resulting in the improvement of coal bioavailability and enhancement of biomethane yield. And some organics with potential to generate methane were released to filtrate as revealed by GC-MS. Our results suggested that NaOH was an effective solution for pretreating coal to enhance biogenic methane production, and anthracite after treating with NaOH could be the better substrate for methanogenesis.

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Biosolubilization of Polish brown coal by Gordonia alkanivorans S7 and Bacillus mycoides NS1020

Cited by 54 publications

References 22 publications

Lignite biodegradation under conditions of acidic molasses fermentation

Lignite biodegradation under conditions of acidic molasses fermentation

Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae

The effect of NaOH pretreatment on coal structure and biomethane production

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