In order to control and remediate arsenic (As) contaminated soil, sediment or water, fungi are used to investigate their potential accumulation and volatilization of As. In this study, after cultivation for 2 days, the dry weights of mycelia for Trichoderma asperellum, Fusarium oxysporum and Penicillium janthinellum all show an increased trend when the As(V) concentration ranges from 0 -50, 0 -50, 0 -80 mg/L, respectively. When the culture system is loaded with 2500 lg As(V), which represents 50 mg/L As, and cultivated for 5 days, P. janthinellum presents the highest efficiency of 87.0 lg/g for As bioaccumulation, and the order of the efficiency for As bioaccumulation is P. janthinellum A T. asperellum A F. oxysporum. However, the order of the amount of volatilized As is F. oxysporum A P. janthinellum A T. asperellum, and the highest amount of volatilized As is observed for F. oxysporum at 181.0 lg. Thus, the ability of As bioaccumulation and biovolatilization for T. asperellum and P. janthinellum is reported for the first time in this study.