Biosorption is the attractive technique of wastewater treatment to retain the dyes. Biosorption of Malachite Green (MG) using leaf biomass Plumbago Zeylanica (PZ) from an aqueous solution was studied using the batch technique. The batch analysis was accessed by altering the variables like time, pH, dye concentration, biosorbent dosage, average biosorbent size and temperature. The % removal of 93% was attained at 25mg/L of concentration for 50ml of an aqueous solution with a time of 45min, pH 9, the dosage of 0.1g and average biosorbent size of 63 µm having 303 K as temperature. By utilizing four models the equilibrium data were reviewed. The Langmuir model was the best fit with an R 2 value of 0.998. The kinetics data were evaluated by three models. The data was suitable to Pseudo-second order kinetics. Thermodynamic parameters evaluated are enthalpy change (∆H o ), entropy change (∆S o ) and free energy (∆G o ). The negative value of (∆H o ) represents an exothermic reaction. The operating conditions are optimized using Response Surface Methodology (RSM)