Biosorption of Heavy Metals from Aqueous Solution by Bacteria Isolated from Contaminated Soil

Abstract

This study was carried out to analyze the heavy metals biosorption potential of bacteria isolated from soil contaminated with electroplating industrial effluents. Bacterial isolates were screened for their multi-metal biosorption potential against copper, nickel, lead, and chromium. Bacterial isolate CU4A showed the maximum uptake of copper, nickel, lead, and chromium in aqueous solution, with a biosorption efficiency of 87.16 %, 79.62%, 84.92%, and 68.12%, respectively. The bacterial strain CU4A was identified as Bacillus cereus, following 16S rRNA gene sequence analysis. The surface chemical functional groups of bacterial biomass were identified by Fourier transform infrared (FTIR) spectroscopy as hydroxyl, carboxyl, amine, and halide, which may be involved in the biosorption of heavy metals. Analysis with scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) confirmed the adsorption of metals on the bacterial cell mass. The results of this study are significant and could be further investigated for the removal of heavy metals from contaminated environments.

Structural Changes of Bacillus subtilis Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

“…Similar changes in the FTIR spectrum due to arsenic biosorption were reported by Cristobel (Christobel and Lipton 2015). Changes at 3411 cm -1 , 2929 cm -1 , 1239 cm -1 , 1052 cm -1 , and 617 cm -1 peak intensities are in line with other reports (Dhanwal et al 2018).…”

Bioremoval of Lead by Bacillus amyloliquefaciens Mpa 1034 Isolated from Wastewater Textile Factories at El-Mahala El-Kobra City, Egypt

“…8 Analysis with SEM and EDX in previous studies confirmed the adsorption of metals on the bacterial biomass. For instance, SEM images of Gram-positive bacteria Bacillus cereus FIT10 (Dhanwal et al,2018) and Staphylococcus hominis strain AMB-2 (Rahman et al,2019) showed clear adsorption of Pb after heavy metals treatment. Moreover, EDX analysis also confirmed the presence of a Pb signal on the bacterial surface.…”

Escherichia colitemplated iron oxide biomineralization under oscillation

“…Bacteria were cultured as previously described. 20,21 Briey, single bacterial colony was inoculated in 3-5 ml of Luria-Bertani (LB) medium, followed by shaking overnight at 37 C at 220 rpm. Cell suspension was inoculated in LB medium at a ratio of 1 : 50, followed by shaking at 220 rpm at 37 C for 12-18 hours.…”

“…Chemical groups such as hydroxyl, carboxyl, amine and halide on bacterial surface are involved in adsorption and deposition of heavy metal ions as well as morphogenesis of biominerals. [19][20][21][22][23] It inspired researchers in using bacterial-mediated mineralization for synthesizing materials with applications in ecological restoration, sewage treatment, drug carrier, etc. [24][25][26] Factors such as temperature, pH, ion strength, biological templates and regulators have been extensively explored in biomineralization.…”