Biosorption of silver cations onto Lactococcus lactis and Lactobacillus casei isolated from dairy products

Milanowski, Maciej; Pomastowski, Paweł; Rafińska, Katarzyna; Ligor, Tomasz

doi:10.1371/journal.pone.0174521

Cited by 26 publications

(9 citation statements)

References 70 publications

(70 reference statements)

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“…The synthesis of ZnO nanomaterials can be carried out by using various types of biological sources such as proteins, plant extracts or microorganisms. Recent studies have demonstrated that both intra- and extracellular approaches are efficient enough to synthesize different types of nanoparticles, e.g., silver [ 10 , 27 , 32 ], gold [ 33 ] or titanium oxide [ 34 ] NPs; however, the microbial synthesis of nan-ZnO has still not been sufficiently described.…”

Section: Resultsmentioning

confidence: 99%

“…For the extracellular synthesis of ZnO NCs, the Lactobacillus paracasei LPC20, from the collection of Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, isolated from sweet whey (Dairy Cooperative in Drzycim, Drzycim, Poland) [ 27 ], was chosen. The L. paracasei LPC20 strain was grown on Müller–Hinton broth (MH, Sigma-Aldrich, St. Louis, MO, USA) and incubated at 37 °C for 24 h. After the incubation, the culture was centrifuged at 12,000 rpm for 30 min and the cell-free supernatant was collected and used for further ZnO NC synthesis.…”

Section: Methodsmentioning

confidence: 99%

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Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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This research presents, for the first time, the potential of the Lactobacillus paracasei LC20 isolated from sweet whey as a novel, effective and accessible source for post-cultured ZnO nanocomposites synthesis. The obtained nanocomposites were subjected to comprehensive characterization by a broad spectrum of instrumental techniques. Results of spectroscopic and microscopic analysis confirmed the hexagonal crystalline structure of ZnO in the nanometer size. The dispersion stability of the obtained nanocomposites was determined based on the zeta potential (ZP) measurements—the average ZP value was found to be −29.15 ± 1.05 mV in the 7–9 pH range. The ZnO nanocomposites (NCs) demonstrated thermal stability up to 130 °C based on the results of thermogravimetric TGA/DTG) analysis. The organic deposit on the nanoparticle surface was recorded by spectroscopic analysis in the infrared range (FT-IR). Results of the spectrometric study exhibited nanostructure-assisted laser desorption/ionization effects and also pointed out the presence of organic deposits and, what is more, allowed us to identify the specific amino acids and peptides present on the ZnO NCs surfaces. In this context, mass spectrometry (MS) data confirmed the nano-ZnO formation mechanism. Moreover, fluorescence data showed an increase in fluorescence signal in the presence of nanocomposites designed for potential use as, e.g., biosensors. Despite ZnO NCs’ luminescent properties, they can also act as promising antiseptic agents against clinically relevant pathogens. Therefore, a pilot study on the antibacterial activity of biologically synthesized ZnO NCs was carried out against four strains (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa) by using MIC (minimal inhibitory concentration). Additionally, the colony forming units (CFU) assay was performed and quantified for all bacterial cells as the percentage of viable cells in comparison to a control sample (untreated culture) The nanocomposites were effective among three pathogens with MIC values in the range of 86.25–172.5 μg/mL and showed potential as a new type of, e.g., medical path or ointment formulation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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“…The Lactobacillus paracasei and Lactococcus lactis strains were isolated from dairy products (Dairy Cooperative in Drzycim, Poland), according to Milanowski et al [50]. The 16S rDNA nucleotide sequences of L. paracasei LB3 and L. lactis 56 have been submitted in GenBank and deposited in the Polish Collection of Microorganisms (PCM) under deposit no.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions

Rogowska

Pomastowski

Walczak

et al. 2019

Toxins

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The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, Lactobacillus paracasei, Lactococcus lactis, and yeast Saccharomyces cerevisiae, isolated from food products, were examined. Moreover, the influence of cellular stress (induced by silver nanoparticles) and lyophilization on the effectiveness of tested microorganisms was also investigated. The concentration of zearalenone after a certain time of incubation with microorganisms was determined using high-performance liquid chromatography. The maximum sorption effectiveness for L. paracasei, L. lactis, and S. cerevisiae cultured in non-stress conditions was 53.3, 41.0, and 36.5%, respectively. At the same time for the same microorganisms cultured at cellular stress conditions, the maximum sorption effectiveness was improved to 55.3, 47.4, and 57.0%, respectively. Also, the effect of culture conditions on the morphology of the cells and its metabolism was examined using microscopic technique and matrix-assisted laser desorption ionization-time of flight mass spectrometry, respectively.

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“…It has been reported that some lactic acid bacteria (LAB) strains, including L. rhamnosus , L. plantarum , and Leuconostoc mesenteroides, are capable of binding and removing heavy metals, such as silver, cadmium and lead in vitro [ 19 , 20 , 21 ]. Furthermore, various recent studies have revealed that LAB can effectively protect against metal-induced oxidative stress through antioxidation in vivo [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 for the Alleviative Effect on Lead Toxicity

Jin

et al. 2017

Nutrients

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Lead (Pb) is a toxic contaminating heavy metal that can cause a variety of hazardous effects to both humans and animals. In the present study, Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 (L. bulgaricus KLDS1.0207), which has a remarkable Pb binding capacity and Pb tolerance, was selected for further study. It was observed that the thermodynamic and kinetic model of L. bulgaricus KLDS1.0207 Pb binding respectively fit with the Langmuir–Freundlich model and the pseudo second-order kinetic model. Scanning electron microscopy and energy dispersive spectroscopy analysis disclosed that the cell surfaces were covered with Pb and that carbon and oxygen elements were chiefly involved in Pb binding. Combined with Fourier transform infrared spectroscopy analysis, it was revealed that the carboxyl, phosphoryl, hydroxyl, amino and amide groups were the main functional groups involved in the Pb adsorption. The protective effects of L. bulgaricus KLDS1.0207 against acute Pb toxicity in mice was evaluated by prevention and therapy groups, the results in vivo showed that L. bulgaricus KLDS1.0207 treatment could reduce mortality rates, effectively increase Pb levels in the feces, alleviate tissue Pb enrichment, improve the antioxidant index in the liver and kidney, and relieve renal pathological damage. Our findings show that L. bulgaricus KLDS1.0207 can be used as a potential probiotic against acute Pb toxicity.

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Biosorption of silver cations onto Lactococcus lactis and Lactobacillus casei isolated from dairy products

Cited by 26 publications

References 70 publications

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions

In Vitro and In Vivo Evaluation of Lactobacillus delbrueckii subsp. bulgaricus KLDS1.0207 for the Alleviative Effect on Lead Toxicity

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