Biospecific Adsorbents on the Basis of Chloroformate-Activated Bead Cellulose

Abstract

Carbohydrate-derived polymers are activated by the chloroformate N-chlorocarbonyloxy-5-norbornene-2.3-dicarboximide (ClCOONB). The advantages of this activation method are presented. The application of bead cellulose as adsorbent for biomedical and biotechnological purposes is demonstrated. Examples for immunoglobulin purification, streptavidin isolation, and biotransformation of porcine insulin are given.

Selective Removal of Circulating Immune Complexes from Patient Plasma

“…In preliminary investigations with systems such as hIgG/anti‐hIgG, HSA/anti‐HSA, and transferrin/antitransferrin, the conditions of ONB‐activation and immobilization of antigen‐antibody complex as well as single antigen or antibody of dissociated immune complexes were analyzed (7–11). These experiments are the basis for the results presented.…”

“…The activation of sepharose was carried out with Cl‐CO‐ONB (5‐norbornene‐2.3‐dicarboximido carbonochloridate) in both a noncatalyzed and a base‐catalyzed manner (7–11). Chromatographic analyses were performed using the ÄKTAexplorer 100 (Pharmacia Biotech Europe, Freiburg, Germany) including the software Unicorn version 2.10.…”

“…The individualized adsorber filling consists of a carrier material derivatized with a protein A purified patient plasma fraction as ligand (patient‐specific immunoadsorber, PsIA) (6). The selection of a suitable carrier material, optimization of the activation method (ONB‐activation) (7–10), and immobilization of cleaved antigen‐antibody complexes on activated support, as well as studies of stability of immobilization (11), model systems such as human IgG/anti‐human IgG, human serum albumin/anti‐human serum albumin, and human transferrin/antitransferrin, were important steps in the development of the PsIA.…”