Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study

Khanal, Dipesh; Kondyurin, Alexey; Hau, Herman; Knowles, Jonathan C.; Levinson, Olga; Ramzan, Iqbal; Fu, Dong; Marcott, Curtis; Chrzanowski, Wojciech

doi:10.1021/acs.analchem.6b00665

Cited by 51 publications

(50 citation statements)

References 75 publications

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“…The absorption is proportional to the cantilever oscillation, enabling a nanomechanical IR absorption spectrum to be derived. This technique has been used mainly in the polymer field [25,31–34], but applications related to biological materials are growing including the mapping of viruses, bacteria, and cells [25,35–38], analysis of protein conformation [28,39–40], and studies of tissue composition. For instance, the distribution of lipids was mapped in the stratum corneum [41].…”

Section: Introductionmentioning

confidence: 99%

Dynamic structure and composition of bone investigated by nanoscale infrared spectroscopy

et al. 2018

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Bone is a highly organized tissue in which each structural level influences the macroscopic and microscopic mechanical behavior. In particular, the quantity, quality, and distribution of the different bone components, i.e. collagen matrix and hydroxyapatite crystals, are associated with bone strength or fragility. Common spectroscopic techniques used to assess bone composition have resolutions limited to the micrometer range. In this study, our aims were two-fold: i) to develop and validate the AFM-IR methodology for skeletal tissues and ii) to apply the methodology to sheep cancellous bone with the objective to obtain novel findings on the composition and structure of trabecular packets.To develop the methodology, we assessed spatial and temporal reproducibility using a known homogeneous material (polymethylmethacrylate, PMMA). We verified that the major peak positions were similar and not shifted when compared to traditional Fourier Transform Infrared imaging (FTIRI). When AFM-IR was applied to sheep cancellous bone, the mineral-to-matrix ratio increased and the acid phosphate substitution ratio decreased as a function of tissue maturity. The resolution of the technique enabled visualization of different stages of the bone maturation process, particularly newly-formed osteoid prior to mineralization. We also observed alternating patterns of IR parameters in line and imaging measurements, suggesting the apposition of layers of alternating structure and / or composition that were not visible with traditional spectroscopic methods. In conclusion, nanoscale IR spectroscopy demonstrates novel compositional and structural changes within trabecular packets in cancellous bone. Based on these results, AFM-IR is a valuable tool to investigate cancellous bone at the nanoscale and, more generally, to analyze small dynamic areas that are invisible to traditional spectroscopic methods.

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Section: Introductionmentioning

confidence: 99%

Dynamic structure and composition of bone investigated by nanoscale infrared spectroscopy

et al. 2018

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“…Surface chemistry induced cytotoxicity had many different origins, including de-activation of biomolecules due to specific surface binding, non-specific protein binding and their denaturation (i.e. beta-sheet formation) 4 , membrane perturbation induced temperature/pH changes, and direct release of various toxin 5 . For instance, magnetic iron NPs coated with dendritic guanidines resulted in a similar cell penetration ability as human immunodeficiency virus-1 transactivator (HIV-TAT) peptide 6 .…”

Section: Introductionmentioning

confidence: 99%

Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells

Zhang

Lung

Zhao

et al. 2017

Sci Rep

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We investigated the influence of nanoparticles’ shape on the physiological responses of cells, when they were fed with spherical and needle-shaped PLGA-PEG nanoparticles (the volume of the nanoparticles had been chosen as the fixed parameter). We found that both types of NPs entered cells via endocytosis and upon internalization they stayed in membrane bounded vesicles. Needle-shaped, but not the spherical-shaped NPs were found to induce significant cytotoxicity in the cell lines tested. Our study evidenced that the cytotoxicity of needle-shaped NPs was induced through the lysosome disruption. Lysosome damage activated the signaling pathways for cell apoptosis, and eventually caused DNA fragmentation and cell death. The present work showed that physiological response of the cells can be very different when the shape of the fed nanoparticles changed from spherical to needle-like. The finding suggests that the toxicity of nanomaterials also depends on their shape.

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“…This reaction on the surface of NPs sometimes induces conformational changes in the adsorbed protein ( Saptarshi et al, 2013 ). Protein secondary structure is especially sensitive to interactions with NPs ( Wang et al, 2011 ; Khanal et al, 2016 ). Eventually, proteins with altered conformations are eliminated from the central nervous system as waste proteins.…”

Section: Discussionmentioning

confidence: 99%

Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles

Onoda

Kawasaki

Tsukiyama

et al. 2017

Front. Cell. Neurosci.

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Environmental stimulation during brain development is an important risk factor for the development of neurodegenerative disease. Clinical evidence indicates that prenatal exposure to particulate air pollutants leads to diffuse damage to the neurovascular unit in the developing brain and accelerates neurodegeneration. Maternal exposure to carbon black nanoparticles (CB-NPs), used as a model for particulate air pollution, induces long-lasting diffuse perivascular abnormalities. We aimed to comprehensively characterize the perivascular abnormalities related to maternal NPs exposure using Fourier transform infrared microspectroscopy (in situ FT-IR) and classical staining analysis. Pregnant ICR mice were intranasally treated with a CB-NPs suspension (95 μg/kg at a time) on gestational days 5 and 9. Brains were collected 6 weeks after birth and sliced to prepare 10-μm-thick serial sections. Reflective spectra of in situ FT-IR were acquired using lattice measurements (x-axis: 7, y-axis: 7, 30-μm apertures) around a centered blood vessel. We also performed mapping analysis of protein secondary structures. Serial sections were stained with using periodic acid-Schiff or immunofluorescence to examine the phenotypes of the perivascular areas. Peaks of amide I bands in spectra from perivascular areas were shifted by maternal NPs exposure. However, there were two types of peak-shift in one mouse in the exposure group. Some vessels had a large peak-shift and others had a small peak-shift. In situ FT-IR combined with traditional staining revealed that the large peak-shift was induced around blood vessel adjacent to astrocytes with glial fibrillary acidic protein and aquaporin-4 over-expression and perivascular macrophages (PVMs) with enlarged lysosome granules. Furthermore, protein secondary structural analysis indicated that maternal NPs exposure led to increases in β-sheet content and decreases in α-helix content in areas that are mostly close to the centered blood vessel displaying histopathological changes. These results suggest that β-sheet-rich waste proteins, which are denatured by maternal NPs exposure, likely accumulate in the perivascular space as they are processed by the clearance systems in the brain. This may in turn lead the denaturation of PVMs and astrocyte activation. The risk of neurodegeneration may be enhanced by exposure to particulate air pollutants during brain development following the perivascular accumulation of β-sheet-rich waste proteins.

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Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study

Cited by 51 publications

References 75 publications

Dynamic structure and composition of bone investigated by nanoscale infrared spectroscopy

Dynamic structure and composition of bone investigated by nanoscale infrared spectroscopy

Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells

Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles

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