Biostimulant activity of humic-like substances from agro-industrial waste on<i>Chlorella vulgaris</i>and<i>Scenedesmus quadricauda</i>

Puglisi, Ivana; Barone, Valeria; Sidella, S.; Coppa, Mauro; Broccanello, Chiara; Gennari, Mara; Baglieri, Andrea

doi:10.1080/09670262.2018.1458997

Cited by 44 publications

(36 citation statements)

References 31 publications

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“…Microalgal growth was conducted in 250 mL flask containing 150 mL of sterile BG11 culture medium [ 37 ] at pH 8.4, incubated on a mechanical shaker (100 rpm) at 25–30 °C, illuminated by a 3500-lx, average photon flux (PPF) 100 μmol photons m −2 s −1 light source (PHILIPS SON-T AGRO 400) with a 12 h photoperiod for 30 days in a growth chamber and aerated by pumps with 20 L h –1 1.5% CO 2 [ 38 ]. Microalgal biomasses were harvested by centrifugation (at 5000 rpm for 15 min), washed with distilled water (up conductivity < 200 μS cm −1 ), and freeze-dried as described in Puglisi et al [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Microalgal Extracts from Chlorella vulgaris and Scenedesmus quadricauda on Germination of Beta vulgaris Seeds

Puglisi

Barone

Fragalà

et al. 2020

Plants

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Sugar beet (Beta vulgaris subsp. vulgaris) is a commercially important biennial root crop, providing about 20% of the world’s annual sugar production. Seed quality is crucial for adequate plant growth and production. The productivity of sugar beet is often limited by heterogeneous germination in the field. In order to improve the sugar beet germination process, the effect of different concentrations of microalgal extracts from Chlorella vulgaris or Scenedesmus quadricauda was investigated by calculating several indices useful to evaluate the germination performance. Moreover, root morphological analysis was performed by using WinRHIZO software. B. vulgaris seeds were soaked with five different concentrations (from 0.1 to 10 mg Corg/L) of the microalgal extracts, considering the amount of organic carbon (Corg) in each extract. Our results show that these microalgal extracts exert a positive effect on sugar beet germination, by increasing efficiency and regularity of this critical process for B. vulgaris seeds. The best results, in terms of germination indices as well as root morphological traits, were reached by using C. vulgaris extract at the concentrations C2 (1 mg Corg/L) and C3 (2 mg Corg/L).

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Section: Methodsmentioning

confidence: 99%

Effect of Microalgal Extracts from Chlorella vulgaris and Scenedesmus quadricauda on Germination of Beta vulgaris Seeds

Puglisi

Barone

Fragalà

et al. 2020

Plants

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“…Chlorella vulgaris (CCAP 211/11C) was obtained and maintained in the algal collection of the Department of Agriculture, Food and Environment (Di3A) (University of Catania, Catania, Italy). C. vulgaris was cultivated as detailed in Puglisi et al [22]. Briefly, microalgae were grown in standard BG11 algae culture medium in a growth chamber, bubbled with air using a pump at around 180 bubbles per minute through a plastic tube fitted to an air regulator, illuminated by a 3500-lux, average photon flux (PPF) 100 µmol m −2 s −1 light source (SON-T AGRO 400, PHILIPS, Eindhoven, the Netherlands), with a 12 h photoperiod (microphotography image is provided in Supplementary Figure S1).…”

Section: Microalgae Culture and Extract Preparationmentioning

confidence: 99%

Foliar Spray Application of Chlorella vulgaris Extract: Effect on the Growth of Lettuce Seedlings

et al. 2021

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Lettuce seedlings often require the use of fertilizers for their cultivation management to achieve appropriate yield. However, for eco-sustainable chemical-fertilizers-free agronomy, the implementation of totally organic farming often cannot support lettuce productivity, therefore new natural biostimulants able to increase lettuce yield could be considered of great interest. In this preliminary work, the foliar spray application of a Chlorella vulgaris extract in lettuce seedlings was investigated in order to achieve better yield performance. Its biostimulant effect was evaluated by monitoring the morphobiometric parameters, chlorophylls, carotenoids, total protein contents, and several enzymatic activities involved in primary and secondary metabolisms of the plant. The experimental trials were carried out by growing lettuce seedlings on inert substrate (pumice) with a 16 h photoperiod for 21 days. The treatment consisted of three consecutive applications by foliar spraying using a concentration of the C. vulgaris extract, corresponding to 1 mg Corg L−1, which were performed one week apart. The results showed that the C. vulgaris extract positively influenced the growth of lettuce seedlings, by increasing the fresh and dry weights, chlorophylls, carotenoids, protein content, and ashes at shoot level. From a biochemical point of view, primary and secondary metabolisms of shoots, in particular nitrogen metabolism, were positively influenced. At the root level, the extract increased dry matter, proteins, and ash content.

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“…The microalgae was cultivated in a growth chamber using standard BG11 algae culture medium [33] bubbled with air and illuminated by a 3500-lux, average photon flux (PPF) 100 µmol m −2 s −1 light source (PHILIPS SON-T AGRO 400) with a 12 h photoperiod [34]. The biomass was obtained by centrifugation and the pellet was washed more times with distilled water to reach a conductivity <200 µS cm −1 [35].…”

Section: Microalgae Culture and Extract Preparationmentioning

confidence: 99%

Biostimulant Effect and Biochemical Response in Lettuce Seedlings Treated with A Scenedesmus quadricauda Extract

Puglisi

Bella

Rovetto

et al. 2020

Plants

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The use of natural biostimulants is becoming an attractive option in order to reduce the use of fertilizer and increase the yield of crops. In particular, algal extracts are suitable candidates as they positively affect plant physiology. Among crops, lettuce often requires the use of biostimulants to improve both the quality and quantity of production. The aim of this work is to investigate the potential use of a Scenedesmus quadricauda extract as a biostimulant in order to obtain sustainable cultivation and a reduction in the cost of chemical fertilizers in lettuce cultivation. Therefore, the effect of S. quadricauda extract on lettuce seedlings was explored by evaluating the physiological parameters, chlorophyll, carotenoid, and total protein contents as well as several plant enzymatic activities involved in primary and secondary metabolisms. The experiment was performed by growing plants on inert substrate (pumice) with a 16-h photoperiod, by carrying out two consecutive radical treatments, one week apart, using a concentration of the extract corresponding to 1 mg Corg L−1. Lettuce plants were sampled at 1, 4, and 7 days from the first treatment and 7 days from the second treatment. The results showed that the S. quadricauda extract positively affected the growth of lettuce seedlings, mainly acting at the shoot level, determining an increase in dry matter, chlorophylls, carotenoids, proteins, and influencing the activities of several enzymes involved in the primary metabolism.

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Biostimulant activity of humic-like substances from agro-industrial waste onChlorella vulgarisandScenedesmus quadricauda

Cited by 44 publications

References 31 publications

Effect of Microalgal Extracts from Chlorella vulgaris and Scenedesmus quadricauda on Germination of Beta vulgaris Seeds

Effect of Microalgal Extracts from Chlorella vulgaris and Scenedesmus quadricauda on Germination of Beta vulgaris Seeds

Foliar Spray Application of Chlorella vulgaris Extract: Effect on the Growth of Lettuce Seedlings

Biostimulant Effect and Biochemical Response in Lettuce Seedlings Treated with A Scenedesmus quadricauda Extract

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