Biosurfactant from <i>Lysinibacillus chungkukjangi</i> from Rice Bran Oil Sludge and Potential Applications

Bhardwaj, Garima; Cameotra, Swaranjit Singh; Chopra, Harish Kumar

doi:10.1007/s11743-016-1857-0

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…In term of performance, biosurfactants produced by Deinococcus caeni PO5 reduced the surface tension of the culture supernatant from 67.0 to 25.0 mN/m after 87 h of cultivation when 40 g/L of jackfruit seed powder and 1 g/L of commercial monosodium glutamate were used as the carbon and nitrogen sources, respectively [ 92 ]. Other than that, a newly discovered bacterium, Lysinibacillus chungkukjangi produced biosurfactant which reduced the surface tension of the media to 27.9 from 72 mN/m when the bacterium was grown on rice bran (by-product of rice milling) [ 93 ]. Rice bran contained high amounts of carbohydrates with 5% of bran which contained 12–18.5% oil [ 94 ].…”

Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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Surfactants are compounds that can reduce the surface tension between two different phases or the interfacial tension of the liquid between water and oil, possessing both hydrophilic and hydrophobic moieties. Biosurfactants have traits that have proven to be advantageous over synthetic surfactants, but these compounds do not compete economically with synthetic surfactants. Different alternatives increase the yield of biosurfactants; development of an economical production process and the usage of cheaper substrates during process have been employed. One of the solutions relies on the suitable formulation of a production medium by including alternative raw materials sourced from agro-wastes, hydrocarbons, or by-products of a process might help in boosting the biosurfactant production. Since the nutritional factors required will be different among microorganisms, the establishment of a suitable formulation for biosurfactant production will be challenging. The present review describes various nutrients and elements considered in the formulation of a production medium with an approach focusing on the macronutrient (carbon, nitrogen source, and C/N ratio), minerals, vitamins, metabolic regulators, and salinity levels which may aid in the study of biosurfactant production in the future.

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Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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“…The effect of reducing the viscosity of oils and the high potential to form emulsions of our biosurfactant can be applied to manufacturing cosmetics (Bhardwaj et al, 2016). However, further studies are required to assess the suitability of this to be used in cosmetics.…”

Section: Viscosity Reductionmentioning

confidence: 99%

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Lima¹,

Andrade²,

RodrÃguez³

et al. 2017

Afr. J. Microbiol. Res.

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The world market for biosurfactants has grown gradually. However, the lack of competitiveness with chemical surfactants due to high cost of production remains a concern. Considering the need to reduce the costs of production, the aim of this work was to study the production and structural characterization of a biosurfactant produced by a strain of yeast Candida glabrata UCP 1556. The lowcost medium containing agro-industrial wastes whey 40% (v/v) and 20% (v/v) corn steep liquor were used as substrates in submerged fermentation. Biosurfactant production was detected by surface tension, oil displacement test and Critical Micelle Concentration (CMC). The structural characterization was performed by Fourier transform infrared (FT-IR), gas chromatography-mass spectrometry (GC-MS) and ionic profile. The stability of emulsions and potential in reducing the viscosity also were investigated. The results showed that the biosurfactant reduced the surface tension to 28.8 mN/m with CMC of 2% and showed anionic profile. Additionally, the biosurfactant formed stable emulsions at temperature (0 to 120°C), pH (2 to 12) and NaCl (2 to 12%), reduced the viscosity of soybean oil (

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“…The DPPH radical scavenging activity of extracts was determined by the procedure mentioned by Bhardwaj et al (2016) with some modifications. 28 0.004% of DPPH (in methanol) and extracts (MES: 0.062 -4.000 mg/mL; MER: 0.400 -120.000 mg/mL) / standard (0.590-302.236 µg/mL) were mixed in the ratio of 1:15 using vortex and kept for 30 min in the dark at RT. After incubation, absorbance was measured by spectrophotometer at 517 nm.…”

Section: Antioxidant Activity Dpph Assaymentioning

confidence: 99%

Phytochemical Screening and Antioxidant Activity Study of Methanol Extract of Stems and Roots of Codonopsis clematidea from Trans-himalayan Region

Bhardwaj¹,

Thakur²,

Kapoor³

et al. 2019

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Aim: The present report aimed to study the therapeutic and phytochemical properties of stems and roots of trans-himalayan plant Codonopsis clematidea. Material and Methods: The crude samples of stems and roots were explored for their chlorophyll, carotenoid and carbohydrate content in order to understand the matrix of these two plant parts. The extraction of phytochemicals was carried out by three different methods viz. sonication, maceration and soxhlet in methanol to identify the best extraction method. Further, analysis of Total Flavonoid Content (TFC) and Total Polyphenolic Content (TPC) were carried out using rutin trihydrate and gallic acid as a reference standard. Antioxidant capacity was estimated using three methods viz. 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay and Ferric Reducing Antioxidant Power (FRAP) assay. In addition to this, GC-MS analysis was also performed for the identification of volatile constituents of Methanol Extract of Stems (MES) and Methanol Extract of Roots (MER). Results: The MES and Dimethylsulfoxide Extract of Stems (DES) were found to have higher Chlorophyll a (Ch a) content in comparison to Chlorophyll b (Ch b) and Carotenoids (C ca). Carbohydrate profile showed that stems and roots have the highest content of fructose in comparison to other sugar moieties. The soxhlet method showed the highest percentage extractive yield in the stems as well as roots. Results revealed that the MES showed higher antioxidant potential as compared to the MER. A correlation has also found to exist between the results of TPC, TFC and antioxidant assays, since TPC and TFC are key constituents responsible for the antioxidant potential. Conclusion: These results have been found to suggest the richness of MES in natural phenols, flavonoids and antioxidants. Further, study should be conducted over identification and characterization of compounds present in the extract.

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Biosurfactant from Lysinibacillus chungkukjangi from Rice Bran Oil Sludge and Potential Applications

Cited by 12 publications

References 25 publications

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Production and characterization of biosurfactant isolated from Candida glabrata using renewable substrates

Phytochemical Screening and Antioxidant Activity Study of Methanol Extract of Stems and Roots of Codonopsis clematidea from Trans-himalayan Region

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