Biosurfactant-producing yeasts widely inhabit various vegetables and fruits

Konishi, Masaaki; Maruoka, Naruyuki; Furuta, Yoshifumi; Morita, Tomotake; Fukuoka, Tokuma; Imura, Tomohiro; Kitamoto, Daï

doi:10.1080/09168451.2014.882754

Cited by 19 publications

(8 citation statements)

References 31 publications

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“…Fifteen additional strains of MEL-producing fungi, representing a variety of Pseudozyma yeasts, were isolated from various vegetables and fruits using this new screening method 18 . 3) .…”

Section: Identification Of Mel-producing Fungimentioning

confidence: 99%

Mannosylerythritol Lipids: Production and Applications

et al. 2015

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“…Fifteen additional strains of MEL-producing fungi, representing a variety of Pseudozyma yeasts, were isolated from various vegetables and fruits using this new screening method 18 . 3) .…”

Section: Identification Of Mel-producing Fungimentioning

confidence: 99%

Mannosylerythritol Lipids: Production and Applications

et al. 2015

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“…18) Isolates were inoculated into 1 mL of YM medium in 96-deep well plates, and cultivated at 25°C with shaking at 1200 rpm for 2 d using a Deep Well Maximizer (Taitec Co., Tokyo, Japan). Culture broth (10 μl) was spotted onto the surface of Parafilm.…”

Section: Microorganismsmentioning

confidence: 99%

Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage

Konishi

Fujita

Ishibane

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Eleven biosurfactant-producing strains were newly isolated from environmental samples using a drop-collapse assay and thin-layer chromatography (TLC). According to the TLC analysis, the separation patterns of the glycolipid spots of nine dominant strains corresponded to that of the sophorolipids produced by a Starmerella bombicola type strain. The retention factor values of the spot patterns of two strains were less than those of the others. Two representative major products were purified, and their molecular structures were determined. The major products were identified as diacetylated lactonic and acidic sophorolipids. The fatty acid moieties of both compounds were estimated to be 17-hydroxymethyl hexadecenoic acid. The amounts of glycolipids ranged from 5.0 to 22.9 g/L after 4 d of cultivation. According to a phylogenetic analysis, the strains were identified as Starmerella bombicola and Candida floricola.

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“…Low biodegradation had been attributed to diverse factors, such as surface soil and effluent properties, chemical toxicity, pollutant concentrations and low availability of the toxicants [3][4][5]. Soil-sorbed PAHs had been considered unavailable for biotransformation without prior desorption, so microorganisms must uptake sorbed molecules or facilitate desorption by surfactant production [6,7].…”

Section: Polycyclicmentioning

confidence: 99%

Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts

Romero¹,

Chiaravalli²,

Reinoso³

2016

Int. J. Biotech. Well. Indus.

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The organic pollutan adsorption/desorption process by microbial degradation had been less studied than metal ones. The sorption assays alone did not predict desorption, due to hysteresis, irreversibility, fixed compounds in different sites, with diverse desorption rates. Most of the studies dealt with bacteria rather than filamentous fungi and yeasts. So, our aims were to isolate yeasts from polluted sediments, to quantify its potential to uptake anthracene (An) and to evaluate the bioavailability by a desorption model. Yeasts were isolated from hydrocarbon-polluted samples, 40isolates grew in anthracene-plates. Molecular characterization was achieved by sequence analysis of the ITS1-5.8S rRNA-ITS4 and 26S rRNA regions; morphological and physiological determination were also done. Candida parasilopsis, Pichia anomala and Rhodothorula mucilaginosa were the prevalent yeasts. An-degradation was assessed in soilsystems with 0, 50, 100, 150, 200 and 250 μg An/l, 3 differentes sorbens types, organic carbon, organic nitrogen, PAHs, sand:silt:clay, pH and cation exchange capacity. Sophorolipids excretion were confirmed by HPLC, UV-detector with active fraction at 9.669 min (RT 9.646 min = sophorolipid-standard). A desorption model with equilibrium, nonequilibrium and nondesorption areas, was applied to explain the experimental data, An-transformation was greater in the organic liquid-phase than in the soil-sorbed ones; the desorption-coefficients and soil components were negatively correlated with the kinetic parameters. The An-release depended on the sophorolipid excretion, soil matrix and particles sizes. Desorption parameters significantly fitted the yeast uptake, with R 2 = 0.97, R 2 = 0.90 and R 2 = 0.97 for C. parasilopsis, P. anomala and R. mucilaginosa, respectively.

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Biosurfactant-producing yeasts widely inhabit various vegetables and fruits

Cited by 19 publications

References 31 publications

Mannosylerythritol Lipids: Production and Applications

Mannosylerythritol Lipids: Production and Applications

Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage

Sorbed Anthracene Degradation by Sophorolipid Producing Yeasts

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