Biosurfactant production by Pseudomonas aeruginosa SP4 using sequencing batch reactors: Effect of oil-to-glucose ratio

Pansiripat, Sira; Pornsunthorntawee, Orathai; Rujiravanit, Ratana; Kitiyanan, Boonyarach; Somboonthanate, Pastra; Chavadej, Sumaeth

doi:10.1016/j.bej.2009.12.011

Cited by 60 publications

(36 citation statements)

References 36 publications

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“…; Pansiripat et al . ), and this partially purified product was further purified by different chromatographical methods. The distinct rhamnolipid spots on preparative TLC plates were then checked further for purity by high‐performance liquid chromatographical analysis.…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D

2013

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Aim: To improve biosurfactant production economics by the utilization of potential low-cost materials. Methods and Results:In an attempt to utilize cost-effective carbon sources in the fermentative production of biosurfactants, various pure and waste frying oils were screened by a standard biosurfactant producing strain. Considering the regional significance, easy availability and the economical advantages, waste frying coconut oil was selected as the substrate for further studies. On isolation of more competent strains that could use waste frying coconut oil efficiently as a carbon source, six bacterial strains were isolated on cetyltrimethyl ammonium bromide-methylene blue agar plate, from a soil sample collected from the premises of a coconut oil mill. Among these, Pseudomonas aeruginosa D was selected as the potential producer of rhamnolipid. Spectrophotometric method, TLC, methylene blue active substance assay, drop collapse technique, surface tension measurement by Du Nouy ring method and emulsifying test confirmed the rhamnolipid producing ability of the selected strain and various process parameters were optimized for the production of maximum amount of biosurfactant. Rhamnolipid components purified and separated by ethyl acetate extraction, preparative silica gel column chromatography, HPLC and TLC were characterized by fast atom bombardment mass spectrometry as a mixture of dirhamnolipids and monorhamnolipids. The rhamnolipid homologues detected were

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Section: Discussionmentioning

confidence: 99%

Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D

2013

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“…The micro‐images of the emulsions from potential surfactant producers were observed through a microscope equipped with a digital camera (Nikon Microscope Eclipse E100; Nikon Corp., Tokyo, Japan). For the supernatant of the potential biosurfactant producers, a drop shape analysis system (Optical contact angle measurement OCAH200; Dataphysics Instrument, filderstadt, Germany) was used to measure the surface tension according to Pansiripat et al. (2010).…”

Section: Methodsmentioning

confidence: 99%

Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil

Chen

Huang

Zhang

et al. 2012

Journal of Applied Microbiology

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Aims: To screen and identify biosurfactant producers from petroleum‐contaminated soil; to use response surface methodology (RSM) for medium optimization to enhance biosurfactant production; and to study the properties of the newly obtained biosurfactant towards pH, temperature and salinity. Methods and Results: We successfully isolated three biosurfactant producers from petroleum‐contaminated soil and identified them through 16S rRNA sequence analysis, which exhibit the highest similarities to Acinetobacter beijerinckii (100%), Kocuria marina (99%) and Kineococcus marinus (99%), respectively. A quadratic response model was constructed through RSM designs, leading to a 57·5% increase of the growth‐associated biosurfactant production by Acinetobacter sp. YC‐X 2 with an optimized medium: beef extract 3·12 g l−1; peptone 20·87 g l−1; NaCl 1·04 g l−1; and n‐hexadecane 1·86 g l−1. Biosurfactant produced by Acinetobacter sp. YC‐X 2 retained its properties during exposure to a wide range of pH values (5–11), high temperatures (up to 121°C) and high salinities [up to 18% (w/v) Na+ and Ca2+], which was more sensitive to Ca2+ than Na+. Conclusions: Two novel biosurfactant producers were isolated from petroleum‐contaminated soil. Biosurfactant from Acinetobacter sp. YC‐X 2 has good properties to a wide range of pH, high temperature and high salinity, and its production was optimized successfully through RSM. Significance and Impact of the Study: The fact, an increasing demand of high‐quality surfactants and the lack of cost‐competitive bioprocesses of biosurfactants for commercial utilization, motivates researchers to develop cost‐effective strategies for biosurfactant production through isolating new biosurfactant producers with special surface‐active properties and optimizing their cultural conditions. Two novel biosurfactant producers in this study will widen our knowledge about this kind of micro‐organism. This work is the first application of RSM designs for cultural optimization of biosurfactant produced by Acinetobacter genus and the first report that biosurfactant may be more sensitive to Ca2+ than Na+.

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“…A 2 4 full factorial experimental design was used, with three central points to determine error, made up of 19 experiments ( Table 1). The factors studied were select based on the literature [4,[24][25][26][27][28]. The minimum and maximum ranges of the variables investigated and the full experimental plan with respect to their values are listed in Table 1.…”

Section: Effect Of Variables On Surface Tensionmentioning

confidence: 99%

Biosurfactant production by Pseudomonas aeruginosa MSIC02 in cashew apple juice using a 24 full factorial experimental design

Rocha

Mendes

Giro

et al. 2014

CI&CEQ

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In this work, the production of biosurfactants from cashew apple juice by Pseudomonas aeruginosa MSIC02 was investigated by carrying out a 2 4 full factorial experimental design, using temperature, glucose concentration from cashew apple juice, phosphorous concentration and cultivation time as variables. The response variable was the percentage of reduction in surface tension in the cell-free culture medium, since it indicates the surface-active agent production. Maximum biosurfactant production, equivalent to a 58% reduction in surface tension, was obtained at 37 °C, with glucose concentration of 5.0 g/L and no phosphorous supplementation. Surface tension reduction was significant, since low values were observed in the cell-free medium (27.50 dyn/cm), indicating that biosurfactant was produced. The biosurfactant emulsified different hydrophobic sources and showed stability in the face of salinity, exposure to high temperatures and extreme pH conditions. These physiochemical properties demonstrate the potential for using biosurfactants produced by P. aeruginosa MSIC02 in various applications.

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Biosurfactant production by Pseudomonas aeruginosa SP4 using sequencing batch reactors: Effect of oil-to-glucose ratio

Cited by 60 publications

References 36 publications

Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D

Production and characterization of rhamnolipid biosurfactant from waste frying coconut oil using a novel Pseudomonas aeruginosa D

Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil

Biosurfactant production by Pseudomonas aeruginosa MSIC02 in cashew apple juice using a 24 full factorial experimental design

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