Biosurfactant trehalose lipid‐enhanced ultrasound‐assisted micellar extraction and determination of the main antioxidant compounds from functional plant tea

Abstract

Hydrosoluble trehalose lipid (a biosurfactant) was employed for the first time as a green extraction solution to extract the main antioxidant compounds (geniposidic acid, chlorogenic acid, caffeic acid, and rutin) from functional plant tea (Eucommia ulmoides leaves). Single‐factor tests and response surface methodology were employed to optimize the extraction conditions for ultrasound‐assisted micellar extraction combined with ultra‐high‐performance liquid chromatography in succession. A Box‐Behnken design (three‐level, three‐factorial) was used to determine the effects of extraction solvent concentration (1–5 mg/mL), extraction solvent volume (5–15 mL), and extraction time (20–40 min) at a uniform ultrasonic power and temperature. In consequence, the best analyte extraction yields could be attained when the trehalose lipid solution concentration was prepared at 3 mg/mL, the trehalose lipid solution volume was 10 mL and the extraction time was set to 35 min. In addition, the recoveries of the antioxidants from Eucommia ulmoides leaves analyzed by this analytical method ranged from 98.2 to 102%. These results indicated that biosurfactant‐enhanced ultrasound‐assisted micellar extraction coupled with a simple ultra‐high‐performance liquid chromatography method could be effectively applied in the extraction and analysis of antioxidants from Eucommia ulmoides leaf samples.

Dietary Eucommia ulmoides Extract Alleviates the Effect of Cold Stress on Chick Growth Performance, Antioxidant and Immune Ability

“…The identification of Eucommia ulmoides and their extracts have been a hot spot in the research and utilization of Eucommia ulmoides. Our study showed that the water extract of EUL contained lignans, iridoids, phenylpropanoids, flavonoids, and so on, which is consistent with that extracted by organic solvent [52,53]. However, the water extract has the advantages of low cost, simple operation, and less pollution.…”

Nanomicellar Extraction of Polyphenols—Methodology and Applications Review

“…The authors of discussed works have chosen among the classic compounds, i.e., cationic CTAB, anionic SDS, or the particularly popular non-ionic compounds such as Triton X-100, Triton X-114, or Genapol X-080. The use of ionic liquids (the IL-type surfactants), i.e., decylguanidinium chloride [ 66 ], C 16 C 4 Im-Br [ 67 ], C 12 mim-Cl [ 68 ], and biosurfactants, i.e., trehalose lipid [ 69 ], hyodeoxycholic acid sodium salt [ 70 ], or synthetic sugar surfactant APG0810 [ 71 ] are still the innovations in this field.…”

“…The authors related this effect to the position of the hydroxyl groups in the sodium salt of hyo -deoxycholic acid. Some substitutions promote hydrogen bond formation and electrostatic interactions with analytes that further increase the mass transfer of the target analytes from sample powder to the aqueous phase [ 69 ]. Therefore, the hydroxyl group at the C6 position is quite unique when compared to the bile salts of human origin.…”

“…In addition, the efficiencies for the other surfactants (α-CD, β-CD, γ-CD, HP-β-CD, and DIME-β-CD) or typical solvents such as ethanol, methanol, or ultrapure water were also not better than that of trehalose lipid. The research clearly shows that biosurfactants are better than methanol and ethanol, and slightly better than water [ 69 ]. In our opinion, the good performance of trehalose lipids was rather the result of its greatest hydrophobicity, but it is difficult to prove because of lack of data.…”

Solvent‐free mixed micellar mobile phases: An advanced green chemistry approach for reversed‐phase HPLC determination of some antihypertensive drugs

“…Surfactants in micellar mobile phases modify stationary phases by masking silanol groups that in turn resulted in improving the peak shape. Surfactants had been applied in determination of drug mixtures when mixed with a small proportion of organic solvent [3][4][5][6][7], applied as green extraction technique [8,9], or sometimes applied as stationary phase replacement (pseudo-phase) [10,11].…”