Biosurfactants from Trichoderma Filamentous Fungi—A Preliminary Study

Piegza, Michał; Pietrzykowska, Joanna; Trojan‐Piegza, Joanna; Łaba, Wojciech

doi:10.3390/biom11040519

Cited by 13 publications

(10 citation statements)

References 20 publications

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“…The results of the diffusion tests performed revealed a great potential to inhibit the development of pathogenic fungi concerning the proportion of surfactants present in the tested post-culture fluids. The preceding separation of the obtained post-culture fluids into P, F, and FR fractions allowed us to initially determine the inhibitory potential of the tested biosurfactants against phytopathogens, rather than their characteristics, as they were obtained in earlier studies ( Piegza et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…The research involved filamentous fungi from the collection of the Department of Biotechnology and Food Microbiology of the Wrocław University of Environmental and Life Sciences: T. citrinoviride C1, T. citrinoviride HL, and T. citrinoviride B3 ( Piegza et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Cultures were carried out in 250 ml Erlenmeyer flasks containing 50 ml of medium. Each tested strain was cultured in Mineral-Glucose-Peptone (MGP) (M) medium ( Piegza et al, 2021 ) or Saunders medium (S), optionally supplemented with 6 g/L yeast biomass (dry biomass of Saccharomyces sp. and Yarrowia sp.…”

Section: Methodsmentioning

confidence: 99%

“…Surface tension measurements were performed immediately after the collection of culture fluids. For this purpose, the tear-off method was used, based on the measurement of force necessary to detach the ring from the surface of the liquid ( Rakowska and Porycka, 2009 ; Piegza et al, 2021 ). The measurements were performed on a Krüss K6 manual tensiometer, at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The complexity of biocontrol mechanisms means that the discovery of any additional factor that may affect the correlation between organisms inspires thorough study. Such a factor was the confirmation that T. citrinoviride strains are capable of biosurfactants biosynthesis ( Piegza et al, 2021 ).…”

Section: Introductionmentioning

confidence: 97%

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Trichoderma citrinoviride: Anti-Fungal Biosurfactants Production Characteristics

Piegza

Szura

Łaba

2021

Front. Bioeng. Biotechnol.

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The mechanism of direct impact of Trichoderma fungi on other organisms is a multilayer process. The level of limiting the growth of other microorganisms is determined by the strain and often by the environment. Confirmation of the presence of extracellular biosurfactants in certain strains of Trichoderma considered as biocontrol agents was regarded as a crucial topic complementing the characterization of their interactive mechanisms. Selected strains of T. citrinoviride were cultured in media stimulating biosurfactant biosynthesis, optionally supplemented with lytic enzyme inducers. Results confirmed the anti-fungal properties of surface-active compounds in the tested culture fluids. Preparations that displayed high fungal growth inhibition presented marginal enzymatic activities of both chitinases and laminarinases, implying the inhibitory role of biosurfactants. Fractions from the foam of the culture fluid of the C1 strain, cultured on Saunders medium, and HL strain on MGP medium, without an additional carbon source, exhibited the most prominent ability to inhibit the growth of phytopathogens. Filamentous fungi capable of producing fungicidal compounds, including surfactants, may find applications in protecting the plants against agri-food pathogenic molds.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Trichoderma citrinoviride: Anti-Fungal Biosurfactants Production Characteristics

Piegza

Szura

Łaba

2021

Front. Bioeng. Biotechnol.

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Prospective use of a cell‐free supernatant from the biosurfactant‐producing marine Planococcus maritimus SAMP MCC 3013 for oil removal and enhanced oil recovery

Waghmode,

Sharma,

Joshi

et al. 2023

Environmental Quality Mgmt

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The cell‐free supernatant (CFS) of the biosurfactant (BS) producing pure strain of Planococcus maritimus SAMP MCC 3013 was used for enhanced oil recovery (EOR). An indigenous marine culture of P. maritimus was grown in mineral salt medium (MSM) supplemented with glucose (1%) (w/v) (30°C, 120 rpm, 7 days). CFS was used to demonstrate its application for oil recovery through three assays: pumice stone, saturated gravel, and the glass beads‐packed column. The recovery of light paraffin oil was ∼40% in the pumice stone assay and ∼43% in the oil‐saturated gravel assay, respectively. The glass bead column demonstrated ∼42% of additional 2T engine oil recovery by CFS. Our study showed that BS containing CFS from P. maritimus SAMP MCC 3013 has the ability to recover oil in its crude form without using any sophisticated purification techniques. All three methods operated by us are harmonizing with each other with respect to the findings. Those methods can be employed in large scale operations for the bio‐recovery of oils using an environmentally friendly approach through the functional properties of BS. This is most likely the first report describing the use of a glass bead pack column for EOR applications particularly from Planococcus sp. These results are symbolic of prospective applications of BS containing CFS from indigenous P. maritimus SAMP MCC 3013 under ex‐situ conditions.

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Optimized biosynthesis of lytic enzymes by special Trichoderma citrinoviride

Piegza,

Łaba

2024

Environ Sci Pollut Res

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The use of Trichoderma filamentous fungi in the wide concept of biocontrol is still a highly relevant topic. The multifaceted nature of their impact on phytopathogenic microorganisms results from the species diversity and complexity of their antagonistic action. The presented research aimed to determine optimal cultivation conditions of two T. citrinoviride strains for the biosynthesis of major enzymes especially those involved in the biocontrol process. Culture conditions were optimized using a three-factor Box-Behnken design to maximize the yield of chitinase and lichenase. The following independent variables were included in the model: incubation temperature, initial pH, and supplementation with fungal biomass. As a result of statistical optimization, unprecedented activities of extracellular lytic enzyme were achieved. For the B1 and B3 strains, the optimal pH was 3.5 or 7.5, respectively, in the determination of chitinase biosynthesis. It was similar for the biosynthesis of β-1.3 and β-1.4 glucanases, but at higher cultivation temperature. The exception was the B3 strain, for which the optimal pH in glucanase biosynthesis was 5.5. The most stimulating culture temperature in the process of chitinase biosynthesis and β-1.3 and β-1.4 glucanases was above 25 °C. In that, the levels of enzyme biosynthesis and corresponding composition culture environment were confirmed to be strain-dependent.

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Biosurfactants from Trichoderma Filamentous Fungi—A Preliminary Study

Cited by 13 publications

References 20 publications

Trichoderma citrinoviride: Anti-Fungal Biosurfactants Production Characteristics

Trichoderma citrinoviride: Anti-Fungal Biosurfactants Production Characteristics

Prospective use of a cell‐free supernatant from the biosurfactant‐producing marine Planococcus maritimus SAMP MCC 3013 for oil removal and enhanced oil recovery

Optimized biosynthesis of lytic enzymes by special Trichoderma citrinoviride

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