Biosurfactants Produced by Yeasts: Fermentation, Screening, Recovery, Purification, Characterization, and Applications

Fernandes, Natalia de Andrade Teixeira; Simões, Luara Aparecida; Dias, Disney Ribeiro

doi:10.3390/fermentation9030207

Cited by 10 publications

(1 citation statement)

References 152 publications

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“…Different Y. lipolytica strains synthesize lipoproteic biosurfactants in the presence of alkanes, which are able to emulsify plant oils (Liposan) or aromatic hydrocarbons (Yasan) [20]. Also, the crude biosurfactant produced by Candida lipolytica UCP 0988 was used for removing 96% of Zn and Cu and reducing the concentration of Pb, Cd, and Fe from barrier silty soil [25], and for removing about 30-40% of Pb and Cu from sand samples [26], respectively.…”

Section: Introductionmentioning

confidence: 99%

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Csutak,

Nicula,

Lungulescu

et al. 2024

Applied Sciences

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The yeast Yarrowia lipolytica degrades petroleum compounds, including alkanes, via the monoterminal oxidation pathway, the hydrophobic carbon substrate assimilation is mediated by biosurfactants, and extracellular amphiphilic molecules are produced by the yeast cell. This study focuses on the ability of the strain Y. lipolytica CMGB32 to degrade n-hexadecane by producing biosurfactants with high potential for bioremediation. The hydrocarbon-degrading potential of the yeast strain was observed via a 2,6-dichlorophenolindophenol (DCPIP) test in Bushnell–Hass medium with 1% n-hexadecane, and cell hydrophobicity was expressed as microbial adhesion to hydrocarbons (MATH). Biosurfactant production on yeast peptone (YP) with 1% n-hexadecane was estimated after 72 h using the emulsification index (E24%) against toluene. Crude biosurfactant (cell-free broth) stability tests were performed at different temperatures (4 °C, 70 °C) and NaCl concentrations (2–10%). The effects of a biosurfactant on synthetic wastewater remediation comprised the growth curves (OD measurements) of natural heavy metal degrader Rhodotorula mucilaginosa, determination of nutrients (spectrophotometrically), physico-chemical parameters, and removal capacity of lead and cadmium ions (via inductively coupled plasma mass spectrometry—ICP-MS). The antimicrobial and anti-adherence activities of 20 mg/mL and 40 mg/mL of the biosurfactant against pathogenic Candida krusei strains involved growth observations and the crystal violet microtiter method. The DCPIP decolorization occurred after six days, corresponding to the maximum growth phase of the Y. lipolytica culture. After 72 h, the cells presented high hydrophobicity (82.61% MATH) and stable biosurfactant production (E24% 47%). The crude biosurfactant (5%) increased the growth of R. mucilaginosa strains cultivated on synthetic wastewater cultures contaminated with Pb2+ and Cd2+, increased the conductivity and COD (86%) of the samples, and determined Pb2+ (66%) and Cd2+ (42%) ions reduction. The concentrated biosurfactant inhibited C. krusei growth (70%) and biofilm adherence. In conclusion, Y. lipolytica CMGB32 shows important potential for development of biosurfactant-based technologies for the remediation of heavy-metal- and emerging pathogen-contaminated wastewaters.

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Section: Introductionmentioning

confidence: 99%

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Csutak,

Nicula,

Lungulescu

et al. 2024

Applied Sciences

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Production and characterization of bioemulsifier from local isolate of Saccharomyces cerevisiae strain JZT351: Antioxidant and antimicrobial effects

Thraeib,

Altemimi,

Al‐Manhel

et al. 2024

eFood

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The widespread use and circulation of industrial emulsifiers pose significant health risks, compounded by their limited availability and high cost. Consequently, there is growing interest in exploring the potential of natural sources, such as microorganisms like yeast, for emulsifier production. In this study, 25 strains of Saccharomyces cerevisiae were isolated from 17 distinct local sources. The yeasts were characterized based on cell shape, size, and colony morphology using yeast malt agar, followed by morphological, microscopic, and biochemical analyzes. Among the isolates, AC1 from a vinegar starter demonstrated superior bioemulsifier production. Emulsification efficiency tests revealed that AC1 exhibited values of 7.1 cm, 0.71 nm, and 34.50%, outperforming other isolates. The biomass yield was approximately 4.35 g/L. This strain was registered in the gene bank as JZT351 (OR115510) after a 100% match with S. cerevisiae. Optimal bioemulsifier production conditions for JZT351 were identified using a liquid yeast extract peptone dextrose medium, with date juice replacing 75% of the glucose, at pH 5.5, 30°C, and 72 h. The resulting emulsification activity, index, and oil displacement were 8.69 cm, 0.95 nm, and 39.7%, respectively. Fourier‐transform infrared spectroscopy (FTIR) compared the functional groups of the bioemulsifier with those of conventional emulsifiers. Molecular characterization was confirmed by 1H NMR. FTIR spectra revealed bioemulsifier bands at 3443.28, 2929.34, 1656.559, 1534.09, 807.06, and 1656.55 cm−1. At 0.5–5 ppm, signals and spectral frequencies corresponded to a mannoprotein structure. The bioemulsifier exhibited 58.09% antioxidant activity at 10 mg/mL, as assessed by DPPH scavenging. It showed the highest inhibition against Pseudomonas aeruginosa and the least against Candida albicans.

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Chemical and biological evaluation of biosurfactant fractions from Wickerhamomyces anomalus CCMA 0358

Fernandes,

Rose,

Simões

et al. 2023

Appl Microbiol Biotechnol

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Biosurfactants Produced by Yeasts: Fermentation, Screening, Recovery, Purification, Characterization, and Applications

Cited by 10 publications

References 152 publications

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation

Production and characterization of bioemulsifier from local isolate of Saccharomyces cerevisiae strain JZT351: Antioxidant and antimicrobial effects

Chemical and biological evaluation of biosurfactant fractions from Wickerhamomyces anomalus CCMA 0358

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