Biosynthesis and antibacterial activity of silver nanoparticles using Flos Sophorae Immaturus extract

Antibacterial, Antioxidant Activities, GC-MS Analysis and Docking Studies of Guiera senigalensis (L.) Ethanol Leaves Extract

“…The antimicrobial activities of G. senigalensis were further investigated by micro-dilution method to determine the minimum inhibitory concentration (MIC) where the method approved by Parvekar, 3 Cheng, 6 Hussain, 25 Carrol, 26 was adopted with few modifications, 3,6,25,26 For this purpose, stock solutions of G. senigalensis extract 50 mg/ml, Mueller-Hinton broth, and Bacterial strain suspension equivalent to 0.5 MacFarland's standard solution were prepared following standard methods. 6 Serial dilution of (50 mg/ml, 25 mg/ml, 12.5 mg/ml, and 6.25 mg/ml) was made in the tubes containing the broth media except for the last one which inoculated with 100 μl of the solvent to be considered as the negative control and the all different tubes were inoculated with 100 μl of the bacterial suspension. Subsequently, all concentrations that showed no change in color were transferred onto nutrient agar and incubated at 37°C overnight, also the concentration exposed a turbidity was subculture in the agar plate as a confirmatory test, the lowest concentration with no growth of bacteria was considered as MBC.…”

“…2 The increment of inappropriate use of Antimicrobial agents including self-medication leads to exacerbation of infections, 4,5 with a specific high prevalence of MDR infections of bacterial pathogens. 6 Especially the increasing of MDR Gram-negative bacteria lead to significant health problems globally, since the wide use of broad-spectrum Antimcrobial agents such as Penicillins, Fluoroquinolones, Aminoglycosides, and β-lactams, such as; Carbapenems, Monobactam, and Cephalosporins; which usually fail to combat the MDR Gramnegative bacteria, 7 that include E. coli and P. aeruginosa the most prevalent respiratory pathogen in immunocompromised patients. 4 Moreover, the treatment of severe infections is caused by Gram-positive bacteria and since the 1980s the threat of resistant strains has been increased with life-threatening consequences.…”

“…8 The MDR bacteria genetically overcome the available antibiotics stress, generating challenges for the treatment of both Hospital and community-acquired infections. 6 Previous studies stated extensive information about some medicinal plants which possess antibacterial activity that has been scientifically studied and popularly used in folk medicine. [9][10][11][12][13][14] Whereas, there phytochemical constituents with antibacterial activities and therapeutic…”

Antibacterial, Antioxidant Activities and GC-MS Analysis of <i>Dichrostachys cinera</i> (L.) Ethanolic Leaves Extract

“…The increase in the irrational use of Antimicrobial agents leads to exacerbation of infections [4] [5], with a high prevalence of MDR bacterial infections [6]. Especially the MDR Gram-negative bacteria which lead to significant health problems worldwide, since the wide use of broad-spectrum Antimicrobial agents including Penicillins, and other β-lactams, such as: Carbapenems, Monobactam, and Cephalosporins; which usually fail to combat the MDR bacteria [7], including E. coli and P. aeruginosa [4].…”

“…The antimicrobial activities of D. cinera were further investigated by micro-dilu- [28] [29]. For this purpose, stock solutions of D. cinera extract 50 mg/ml, Mueller-Hinton broth and Bacterial strain suspension equivalent to 0.5 MacFarland's standard solution were prepared following standard methods [6]. Serial dilution of (50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml) was made in the tubes containing the broth media except for the last one which inoculated with 100 μl of the solvent to be considered as the negative control and the all different tubes were inoculated with 100 μl of the bacterial suspension.…”