BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN

Semeniuk, Ihor; Koretska, Nataliia; Кочубей, В. В.; Lysyak, Victor; Pokynbroda, Tetyana; Karpenko, Elena; Midyana, G. G.

doi:10.55251/jmbfs.4714

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Since PHA is the intracellular biopolymer, the possibility of obtaining it as a co-product of the synthesis of extracellular and cell-bound biosurfactants was studied. Therefore, actinobacteria R. erythropolis Au-1 and G. rubripertincta UCM Ac-122 were cultivated according to the parameters for obtaining biosurfactants [29,30]. After 120 hours cultivation on nutrient medium (1) the yield of PHA was 2.6% w/w (G. rubripertincta UCM Ac-122) and 4.8 % w/w (R. erythropolis Au-1) from biomass (Tables 1, 2).…”

Section: Resultsmentioning

confidence: 99%

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Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites

Koretska,

Semeniuk,

Pokynbroda

et al. 2023

Innov Biosyst Bioeng

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Background. The accumulation of plastic waste negatively affects the environment and human health. Currently, one of the strategies to address this global ecological problem involves the utilization of biodegradable plastics instead in place of synthetic ones. Among them, polyhydroxyalkanoates (PHA) – microbial intracellular polymers – hold a significant position. Their advantages are biodegradability, biocompatibility, and favorable thermomechanical properties. Given these attributes, PHA has significant prospects for use in medicine, agriculture, and the food industry, in particular for packaging food products. Objective. Enchance the efficiency of bacterial synthesis of polyhydroxyalkanoates through nutrient media modification, obtain antimicrobial composites based on PHA, and determine their antimicrobial properties. Methods. The optimization of PHA biosynthesis involved selecting appropriate cultivation conditions, including carbon and nitrogen sources, cultivation time, and working volume. The isolation of PHA from bacterial biomass was achieved through chloroform extraction (mixing for 10 h at 35 °C, with a biomass-to-chloroform ratio of 1:50); followed by precipitation with double the volume of isopropanol. The resulting polymer was then dried to a constant weight at 60 °C. The hydrophobicity of the biopolymer was assessed using the water contact angle measurement. Composites of biopolymers with antimicrobials in the form of films were obtained using two methods: 1) solution casting method; and 2) layering the biocides onto the polymer film. The antimicrobial activity of the resulting composites was determined using the agar diffusion method. Results. Through the optimization of the mineral media and the change of cultivation conditions, it was possible to obtain 0.26–1.45 g/l of polyhydroxyalkanoates (5.1–34.0% PHA from biomass). The R. ruber UCM Ac-288 strain synthesized the maximum amount of biopolymer (34.0% PHA). This study established the ability of Gordonia bacteria to synthesize PHA for the first time. PHA compositions of optimal content were obtained, with hydrophobicity comparable to that of polyethylene packaging films. Antimicrobial properties of biopolymers composites with biocides have been substantiated. Conclusions. The bacterial synthesis of PHA was increased by modifying nutrient media. Composites based on PHA with biocides were developed. It was determined that these composites exhibit antimicrobial properties and high hydrophobicity. Consequently, they hold promise for use as biofilms for packaging and preserving food products.

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Section: Resultsmentioning

confidence: 99%

“…PHA of A. vinelandii N-15 was identified as polyhydroxybutyrate (PHB) with a melting point of 189 C and the onset of destruction at 294 C [21]. The biopolymer of the R. erythropolis Au-1 strain belongs to polyhydroxyalkanoates with a melting point of 42 C and a destruction temperature of 160 C [29].…”

Section: Resultsmentioning

confidence: 99%

Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites

Koretska,

Semeniuk,

Pokynbroda

et al. 2023

Innov Biosyst Bioeng

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Temperature and physical-mechanical properties of thermoplastic materials based on polyhydroxybutyrate

Semeniuk¹,

Кочубей²,

Skorokhoda³

et al. 2022

Vopr. Khim. Khim. Tekhnol.

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The temperature and physical-mechanical properties of polyhydroxybutyrate obtained during the cultivation of Azotobacter vinelandii N-14 bacteria strain, polylactic acid, poly(-caprolactone) and their blends were studied. Polymer samples in the form of films with a thickness of 50 m were cast on silicate glass plates applying 3% solutions of biopolymers in chloroform and subjected to heat treatment in an air thermostat at the temperature of 1100C for 20 minutes. It was found that the previous heat treatment of the studied biodegradable polymers increases their heat resistance which is evidenced by the shift of their thermal effects of melting to higher temperature area. It was determined that films based on polyhydroxybutyrate/polylactic acid/poly(-caprolactone) (20:55:25 wt.%) blend possesses improved mechanical properties. The film relative elongation at break increases from 3% to 46% for polyhydroxybutyrate and for the mixture, respectively. This is explained by the presence of poly(-caprolactone) polymers in above mixture. At the same time, despite the lower tensile strength characteristic which are typical of poly(-caprolactone), this value is higher for the polymer blends than for polyhydroxybutyrate (38.3 and 33.0 MPa, respectively). This is due to the high polylactic acid content (55%) in the polymer composition. The presence of poly(-caprolactone) (25%) in the blend improves the fluidity of its melt which will enable to reduce the temperature of product formation. The derived polymer mixture is recommended for the manufacturing of various packaging materials for food, agricultural and pharmaceutical products.

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BIOSYNTHESIS AND CHARACTERISTICS OF METABOLITES OF Rhodococcus erythropolis AU-1 STRAIN

Cited by 2 publications

References 21 publications

Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites

Polyhydroxyalkanoates: Biosynthesis Optimization and Design of Antimicrobial Composites

Temperature and physical-mechanical properties of thermoplastic materials based on polyhydroxybutyrate

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