Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source

Abstract

This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore, confirmed to be biocompatible in nature and suitable for neural regeneration.

Comparison of the Influence of 45S5 and Cu-Containing 45S5 Bioactive Glass (BG) on the Biological Properties of Novel Polyhydroxyalkanoate (PHA)/BG Composites

“…P(3HO), P(3HO-co-3HD) and P(3HO-co-3HD-co-3HDD) polymers were produced using Gram-negative bacterium Pseudomonas mendocina CH50, obtained from the National Collection of the Industrial and Marine Bacteria (NCIMB 10541) and different carbon sources (Table 1) based on optimized protocols [41][42][43]. A single colony of this strain was grown under the same conditions and in the same seed culture medium as described for P(3HB).…”

Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses

“…This was incubated for 16 h at 30 • C and at 200 rpm. Inoculum of 10 vol.% of the final volume was used to inoculate the second stage seed culture (mineral salt medium-MSM) which was incubated at 30 • C, 200 rpm for 24 h. Second stage seed culture was used to inoculate the final PHA production media [21,22]. The culture was grown for 48 h, at 30 • C, 200 rpm and air flow rate of 1 vvm.…”

“…pH of the supernatant was measured using Seven Compact pH meter (Mettler Toledo Ltd., Leicester, UK). Nitrogen in the form of ammonium ions was estimated by the phenol-hypochlorite method [21,22].…”

“…The aim of this study was the development of natural antimicrobial polymer films via the incorporation of an essential oil into biodegradable PHA produced from agri-food by-products. We report a feasible way of valorisation of sugarcane molasses for the production of PHA by Pseudomonas mendocina CH50, a bacterial strain which has been previously confirmed as an efficient producer of MCL PHAs [5,20,21]. It was demonstrated that in the presence of sugarcane molasses P. mendocina CH50 was able to accumulate an MCL PHA, which was identified as a copolymer of 3-hydroxyoctanoate and 3-hydroxydecanoate, or P(3HO-co-3HD).…”

Toward a Closed Loop, Integrated Biocompatible Biopolymer Wound Dressing Patch for Detection and Prevention of Chronic Wound Infections

“…MCL-PHAs have monomers consisting of 6–14 carbons such as polyhydroxyundecenoate, PHU and Poly(3-hydroxyoctanoate), P(3HO) ( Dufresne and Vincendon, 2000 ; Rai et al, 2011 ) and these polymers are elastomeric in nature with low crystallinity, low tensile strength, low melting point and high elongation at break ( Nomura and Taguchi, 2007 ). The degradation products of PHAs (hydroxyl acids) have almost no cytotoxicity as compared to the degradation products of synthetic polymers (lactic acid in case of PLA) which leads to low immunogenicity ( Rai et al, 2011 ; Basnett et al, 2018 ; Constantinides et al, 2018 ). This is because the hydroxyl acids such as hydroxybutyric acid, hydroxyoctanoic acid are natural metabolites.…”