Biosynthesis and characterization of chitosan based hydrogel: A potential in vitro wound healing agent

Arulmoorthy, M. P.; Anbarasi, G.; Srinivasan, M.; Vishnupriya, B.

doi:10.1016/j.matpr.2020.07.186

Cited by 8 publications

(8 citation statements)

References 47 publications

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“…Similar behavior was observed for both polymers and against both bacteria. In the first few hours, a bactericidal effect was observed; as suggested by other researchers, the adhesion of chitosan to the cell wall of the bacteria plays a fundamental role in breaking cellular homeostasis and forcing cell death [ 21 ]. However, with the passage of time, both bacteria can proliferate.…”

Section: Resultsmentioning

confidence: 97%

“…As shown in Table 3 , none of the tested microorganisms showed any proliferation. According to other researchers [ 21 ], the positive charge of the chitosan is attributed to the presence of amino groups in the C5 position of their constituent monomers. Their positive charge induces interaction with negative charges on the membranes of microorganisms, altering the usual course of microbial homeostasis, preventing microbiological growth, and conferring bacteriostatic properties.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of the Extraction of Chitosan and Fish Gelatin from Fishery Waste and Their Antimicrobial Potential as Active Biopolymers

Rocha-Pimienta

Navajas-Preciado

Barraso-Gil

et al. 2023

Gels

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Fishery residues are abundant raw materials that also provide numerous metabolites with high added value. Their classic valorization includes energy recovery, composting, animal feed, and direct deposits in landfills or oceans along with the environmental impacts that this entails. However, through extraction processes, they can be transformed into new compounds with high added value, offering a more sustainable solution. The aim of this study was to optimize the extraction process of chitosan and fish gelatin from fishery waste and their revalorization as active biopolymers. We successfully optimized the chitosan extraction process, achieving a yield of 20.45% and a deacetylation degree of 69.25%. For the fish gelatin extraction process, yields of 11.82% for the skin and 2.31% for the bone residues were achieved. In addition, it was demonstrated that simple purification steps using activated carbon improve the gelatin’s quality significantly. Finally, biopolymers based on fish gelatin and chitosan showed excellent bactericidal capabilities against Escherichia coli and Listeria innocua. For this reason, these active biopolymers can stop or decrease bacterial growth in their potential food packaging applications. In view of the low technological transfer and the lack of information about the revalorization of fishery waste, this work offers extraction conditions with good yields that can be easily implemented in the existing industrial fabric, reducing costs and supporting the economic development of the fish processing sector and the creation of value from its waste.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Optimization of the Extraction of Chitosan and Fish Gelatin from Fishery Waste and Their Antimicrobial Potential as Active Biopolymers

Rocha-Pimienta

Navajas-Preciado

Barraso-Gil

et al. 2023

Gels

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“…The scientific publications related to the chitin/chitosan and collagen value chains analysed in the present study focused mostly on how these bioactive compounds and/or their derivatives were obtained rather than on the later steps of both value chains and market-related information. Interestingly, more publications mentioned [70,[149][150][151] the origin of raw materials, inputs/feedstock, and the pre-treatment and pre-processing steps in the collagen value chain than in the chitin/chitosan value chain, but the opposite trend was found [65,110,152] for processing and product manufacturing, consumption, chain outputs, and the interaction between stakeholders. This switch may indicate that the focus of scientific publications related to the chitin/chitosan value chain is changing towards the final steps.…”

Section: Trends In Data Distribution Along Each Value Chainmentioning

confidence: 99%

“…A high percentage (~60%) of publications also mentioned future perspectives for chitin/chitosan and collagen products [68,150,152,153], indicating an interest in developing new products based on these bioactive compounds and/or developing new applications for marine-derived collagen and chitin/chitosan products. This high percentage also reflects the forward-thinking of researchers working on marine-derived chitin/chitosan and collagen and the active pursuing of their full potential for innovative applications, markets niches, and opportunities.…”

Section: Trends In Data Distribution Along Each Value Chainmentioning

confidence: 99%

“…This high percentage also reflects the forward-thinking of researchers working on marine-derived chitin/chitosan and collagen and the active pursuing of their full potential for innovative applications, markets niches, and opportunities. However, most publications only mentioned these innovations as possibilities [150,154], without developing further into which technology readiness level (TRL) the marine-derived chitin/chitosan and collagen products are at or which level they might be at in the near future. This may be due to a lack of engagement between researchers and industry partners/end-users, as well as not considering market demands and needs at the R&D and process development stages.…”

Section: Trends In Data Distribution Along Each Value Chainmentioning

confidence: 99%

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Current and Expected Trends for the Marine Chitin/Chitosan and Collagen Value Chains

Vieira,

Lestre,

Solstad

et al. 2023

Marine Drugs

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Chitin/chitosan and collagen are two of the most important bioactive compounds, with applications in the pharmaceutical, veterinary, nutraceutical, cosmetic, biomaterials, and other industries. When extracted from non-edible parts of fish and shellfish, by-catches, and invasive species, their use contributes to a more sustainable and circular economy. The present article reviews the scientific knowledge and publication trends along the marine chitin/chitosan and collagen value chains and assesses how researchers, industry players, and end-users can bridge the gap between scientific understanding and industrial applications. Overall, research on chitin/chitosan remains focused on the compound itself rather than its market applications. Still, chitin/chitosan use is expected to increase in food and biomedical applications, while that of collagen is expected to increase in biomedical, cosmetic, pharmaceutical, and nutritional applications. Sustainable practices, such as the reuse of waste materials, contribute to strengthen both value chains; the identified weaknesses include the lack of studies considering market trends, social sustainability, and profitability, as well as insufficient examination of intellectual property rights. Government regulations, market demand, consumer preferences, technological advancements, environmental challenges, and legal frameworks play significant roles in shaping both value chains. Addressing these factors is crucial for seizing opportunities, fostering sustainability, complying with regulations, and maintaining competitiveness in these constantly evolving value chains.

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In vitro Models for Predicting Bioadhesion Fracture Strength to Ex Vivo Animal Buccal Tissue

Rahamim,

Patel,

Drori

et al. 2024

Small

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Commitment to the 3Rs principle (Replacement, Reduction, and Refinement) led to the development of a cell‐based system to measure buccal bioadhesion in vitro and replace the use of porcine buccal and esophageal tissues (PBT and PET, respectively). Additionally, the aim is to bridge the gap in knowledge regarding the bioadhesion properties of PBT and PET. The in vitro models are based on the human buccal epithelial cell line—TR146 without (“Model I”) or with (“Model II”) 5% (w/v) mucous layer. The in vitro setup also provides a method to evaluate the bioadhesion between two soft materials. Standard bioadhesive hydrogels (alginate, chitosan, and gelatin) are used to test and compare the results from the in vitro models to the ex vivo tissues. The ex vivo and in vitro models show increased bioadhesion as the applied force and contact time increases. Furthermore, Model I exhibits bioadhesion values—of alginate, chitosan, and gelatin—comparable to those obtained with PBT. It is also found that contact time and applied force similarly affect PBT and PET bioadhesion, while PET exhibits greater values. In conclusion, Model I can replace PBT for measuring bioadhesion and be incorporated into the experimental design of bioadhesive DDS, thus minimizing animal tissue usage.

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Biosynthesis and characterization of chitosan based hydrogel: A potential in vitro wound healing agent

Cited by 8 publications

References 47 publications

Optimization of the Extraction of Chitosan and Fish Gelatin from Fishery Waste and Their Antimicrobial Potential as Active Biopolymers

Optimization of the Extraction of Chitosan and Fish Gelatin from Fishery Waste and Their Antimicrobial Potential as Active Biopolymers

Current and Expected Trends for the Marine Chitin/Chitosan and Collagen Value Chains

In vitro Models for Predicting Bioadhesion Fracture Strength to Ex Vivo Animal Buccal Tissue

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