Biosynthesis and Characterization of Silver Nanoparticles Using Phoenix dactylifera Fruits Extract and their In Vitro Antimicrobial and Cytotoxic Effects

Abstract

Background: In this article, silver nanoparticles (AgNPs) were synthesized by the biological green technique, using the aqueous extracts obtained from fruits of Phoenix dactylifera (date palm). This method is simple, rapid, non-toxic, and sustainable, and substitutes for the conventional physical/chemical methods. The cytotoxic activities of AgNPs derived from date fruit extract have not been mentioned in the earlier studies. Methods: The biosynthesized AgNPs are analyzed by Fourier transform infrared spectroscopy (FT-IR), UV-visible spectroscopy (UV-vis), and Transmission Electron Microscopy (TEM) methods. The assessment of antimicrobial effect towards human pathogenic microbial strains and their potential cytotoxicity against human breast cancer cell lines (MCF-7) were also evaluated. Results: FT-IR spectral studies showed that phytomolecules such as carbohydrates, phenolic acids and flavonoids present in date fruits extract are involved in the reduction and capping of the AgNPs. UV-vis spectrum revealed Surface Plasmon Resonance (SPR) at 425 nm which attributes the presence of AgNPs in aqueous extract. TEM micrographs showed that AgNPs particle diameter is ranged from 20 nm to 100 nm with spherical morphology. The biosynthesized AgNPs exhibited significant antimicrobial activity towards human microbial strains. Phytosynthesized NPs also induce cytotoxicity via necrosis, apoptosis and mitodepressive mechanisms that can disturb the cellular components at various stages of cell cycle. Conclusion: The present study concludes that biologically synthesized AgNPs using Phoenix dactylifera is cost-effective, rapid, non-toxic, and sustainable and can be effectively used as an adjunct for the treatment of breast carcinoma.

Flexible Methyl Cellulose/Polyaniline/Silver Composite Films with Enhanced Linear and Nonlinear Optical Properties

“…The collected SEM images of Figure 4 provided useful information for understanding the structural and the changes of the optical properties of the films under the influence of PANI and AgNPs additions. As can be seen, the SEM image of pure MC film (image a) shows a smooth and uniform surface, while the SEM images of MC/1%PANI and MC/3%PANI films (images b and c) display rough surfaces with the emerge of some small aggregates corresponding to PANI [ 19 , 20 , 21 ]. The SEM images (d and e) show a good distribution of the AgNPs into the MC/PANI films, indicating a sufficient interfacial interaction to MC/PANI blend matrix and the AgNPs [ 22 ].…”

Fruit Extract Mediated Green Synthesis of Metallic Nanoparticles: A New Avenue in Pomology Applications

“…The analysis of cytotoxic potential was done assessing the level of ROS. On the other hand, Phoenix dactylifera mediated AgNPs exhibited dose-dependent cytotoxicity against MCF-7 [ 58 ]. The result obtained from the study showed the highest inhibitory effect at a concentration of 200 μg/mL.…”

Silver nanoparticles from Ziziphus spina-christi (L.) Desf. fruits extract and their antimicrobial activity

“…5). Previous work documented that NPs from fruits extract had antimicrobial activity, for example, the biosynthesized AgNPs fruit extract of Phoenix dactylifera showed substantial antimicrobial activity against human pathogens (Zafar and Zafar 2019). There were AgNps from some fruit varieties of Carica papaya, Punica granatum, Vitis amurensis, Citrus reticulata, Ananas comosus, Vitis vinifera and Ficus carica that showed significant antimicrobial activities (Samrot et al 2018).…”