Biosynthesis and characterization of silver nanoparticles using Agrocybe cylindracea, and their antibacterial activity.

Abstract

Mushrooms are of great interest in nanotechnology. Antibiotics resistance is a serious condition that threatens the treatment of many diseases caused by bacteria. So, it becomes persistent to find new antibacterial agents. Silver nanoparticles (AgNPs) showed a good antibacterial activity and the green synthesis of silver nanoparticles is considered a safe method. In this study AgNPs were biologically synthesized from mycelial free filtrate of an Egyptian mushroom Agrocybe cylindracea. The shift in reaction color from pale yellow to dark brown served as a primary indication for the creation of nanoparticles. The production of AgNPs was further confirmed using UV-visible spectrophotometer and the synthesis process was optimized at different parameters. The best concentration of silver nitrate, pH and temperature values were 4 mM, 7 and 40 °C, respectively. Transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and Zetasizer analyses were used to investigate the characteristics of the produced nanoparticles. TEM studies showed that the size of synthesized AgNPs was 3.47 -13.99 nm. FTIR studies showed the presence of some functional groups (O-H, C-H and C=C) which might be involved in the reduction of silver nitrate to silver ion and stabilization of nanoparticles. Zeta potential of the nanoparticles was -3.57. Antibacterial activity of synthesized AgNPs was demonstrated against different harmful microorganisms; Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae and the best antibacterial activity was recorded against P. aeruginosa.

Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities

“…The compounds mentioned above have a crucial role in the stabilization, capping, and reduction of Ag-NPs, and they may be identified within the cell-free filtrate of A. hiratsukae . These results are consistent with the findings of El-fallal et al (2022) , on Agrocybe Cylindracea and Agrocybe aegerita by Nagalakshmi (2015) .…”

“… Alharbi and Alsubhi (2022) , synthesized Ag-NPs with TEM imaging that were spherical with diameters of 27 nm. Based on a study conducted by El-fallal et al (2022) , the TEM analysis revealed that the AgNPs synthesized by Agrocybe cylindracea exhibited a spherical shape and a range of sizes from 3.47 to 13.99 nm. In a recent study by Nguyen et al (2020) , it was found that the P.uri.…”