Biosynthesis and Characterization of Silver Nanoparticles Produced by Plant Extracts and Its Antimicrobial Activity

Abstract

Solanum tubersum is the fourth most challenging plant in Egypt, affected by several fungi, viral and bacterial diseases. Bacterial and fungal isolates (Brown rot disease (Ralstonia solaniserum), soft root disease (Pectobacterium carotovora) and dry rot disease (Fusarium oxisporum) were collected. The green extracts of silver nanoparticles were prepared by means of aqueous extracts of three wild plants, Physalis peruviana (leaves, red and green fruits) (N1, N2 and N3), Solanum nigrum (fruit) (N4) and Moringa oliefera (leaves) (N5). SEM, TEM, FT-IR and X-RD obtained the characterization of the biosynthesis of silver nanoparticles. The results indicated that nanoparticles were spherical, smooth and the sizes varied between 12 and 33 nm. The activity of the nanoparticle formulations was tested against the bacterial isolates using agar diffusion method and one fungus using mycelial growth method. The results also elucidated that N5 formulation showed a significantly potent antibacterial activity against R. solanacearum. However, N1 formulation was the highest active one against P. carotovra. In addition, the antifungal activity indicated that N1 had the highest effect (EC50 = 687.03 mg/L) followed by N3 (EC50 = 981.61 mg/L) against F. oxysporium. Nanoparticles synthesized by wild plants could be used as safe alternatives to harmful microbicides.

Elicitation of salicylic acid and methyl jasmonate provides molecular and physiological evidence for potato susceptibility to infection by Erwinia carotovora subsp. carotovora

“…carotovora isolates on different potato cultivars. This finding aligns with a previous study where potato parts inoculated with different bacterial isolates exhibited rapid rot and discoloration after 24 h [ 52 ]. A previous research has also confirmed the variation in sensitivity to bacterial soft rot among potato cultivars [ 33 ].…”

Preparation and Characterization the Complexes of Dithiocarbamide Derived from Amino Acids and Evaluation of Their Biological Activity

“…Among the scientist attempts recorded the increasing of secondary metabolites in plants, the content of vanilla plant Vanilla planifolia increased when nano-silver was added to the MS medium, which was nourished with total phenols and glycosidic compounds due to increased production of ROS (Reactive oxygen species) at concentrations of (25 and 50) mg. L‾¹ (Santoscoy , et al 2017). In a study by ( Javed 2017) when growing the sugar leaf plant Stevia rebaudiana outside the living body to check the impact of different concentrations of nano-zinc oxide (0, 0.1, 1.0, 10, 100, and 1000 mg.L‾¹) in the absence of traditional growth regulators on the production of Secondary compounds, as the results showed superiority to the treatment of 1 mg. L‾¹ of nano-copper oxide increased the production of flavonoids and total phenols, while all studied indicators decreased with increasing concentration of nano-copper oxide ( Samy et al 2019) also indicated their success in using silver nanomaterial produced from the herb extract as an alternative to fungicides to control some bacterial and fungal infections affecting potatoes. Silver nanoparticles can act as a catalyst to produce more copies of the genes of the steviol glycoside biosynthesis pathway, thus increasing the production of this compound Between (Ramezani et.…”

Role of Interleukin-35 In The Pathogenesis of Hashimoto’s Thyroiditis Disease

“…Among the scientist attempts recorded the increasing of secondary metabolites in plants, the content of vanilla plant Vanilla planifolia increased when nano-silver was added to the MS medium, which was nourished with total phenols and glycosidic compounds due to increased production of ROS (Reactive oxygen species) at concentrations of (25 and 50) mg. L‾¹ (Santoscoy , et al 2017). In a study by ( Javed 2017) when growing the sugar leaf plant Stevia rebaudiana outside the living body to check the impact of different concentrations of nano-zinc oxide (0, 0.1, 1.0, 10, 100, and 1000 mg.L‾¹) in the absence of traditional growth regulators on the production of Secondary compounds, as the results showed superiority to the treatment of 1 mg. L‾¹ of nano-copper oxide increased the production of flavonoids and total phenols, while all studied indicators decreased with increasing concentration of nano-copper oxide ( Samy et al 2019) also indicated their success in using silver nanomaterial produced from the herb extract as an alternative to fungicides to control some bacterial and fungal infections affecting potatoes. Silver nanoparticles can act as a catalyst to produce more copies of the genes of the steviol glycoside biosynthesis pathway, thus increasing the production of this compound Between (Ramezani et.…”