Biosynthesis and characterization of silver nanoparticles (AgNPs) using marine bacteria against certain human pathogens

Begam, J. Nasrin

doi:10.7439/ijasr.v2i7.3514

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…Bands in the range of 1923–2000 cm −1 prove the existence of an aromatic ring, while bands in the range of 2900–2990 cm −1 correspond to C-H stretching and the broad and intense band near 3400 cm −1 indicates the presence of O−H stretching in phenols [ 64 , 66 , 67 ]. The bands at 510 cm −1 and 590 cm −1 are attributed to the metal-ligand frequency stretching which formed due to the interaction between biomolecules and the AgNPs surfaces [ 68 , 69 ].…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

et al. 2022

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The rapid development of nanotechnology and its applications in medicine has provided the perfect solution against a wide range of different microbes, especially antibiotic-resistant ones. In this study, a one-step approach was used in preparing silver nanoparticles (AgNPs) by mixing silver nitrate with hot Hypericum perforatum (St. John’s wort) aqueous extract under high stirring to prevent agglomeration. The formation of silver nanoparticles was monitored by continuous measurement of the surface plasma resonance spectra (UV-VIS). The effect of St. John’s wort aqueous extract on the formation of silver nanoparticles was evaluated and fully characterized by using different physicochemical techniques. The obtained silver nanoparticles were spherical, monodisperse, face-centered cubic (fcc) crystal structures, and the size ranges between 20 to 40 nm. They were covered with a capping layer of organic compounds considered as a nano dimension protective layer that prevents agglomeration and sedimentation. AgNPs revealed antibacterial activity against both tested Gram-positive and Gram-negative bacterial strains causing the formation of 13–32 mm inhibition zones with MIC 6.25–12.5 µg/mL; Escherichia coli strains were resistant to tested AgNPs. The specific growth rate of S. aureus was significantly reduced due to tested AgNPs at concentrations ≥½ MIC. AgNPs did not affect wound migration in fibroblast cell lines compared to control. Our results highlighted the potential use of AgNPs capped with plant extracts in the pharmaceutical and food industries to control bacterial pathogens’ growth; however, further studies are required to confirm their wound healing capability and their health impact must be critically evaluated.

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Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

et al. 2022

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“…The strong absorption peak around ~450 cm −1 describes the formation of pure and AgNPs-loaded TiO2 nanocrystals. Furthermore, with the increase in concentration of Ag in TiO2, there is a shift in the characteristics peak (~450 cm −1 ) towards higher wavenumbers as shown in the zoomed area of marked region in Figure 2, and the occurrence of new peaks corresponding to AgNPs (~1400 cm −1 ) were observed [53,54].…”

Section: Ftir Analysismentioning

confidence: 85%

An Experimental and Theoretical Study on the Effect of Silver Nanoparticles Concentration on the Structural, Morphological, Optical, and Electronic Properties of TiO2 Nanocrystals

et al. 2021

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In this work, pure and silver (Ag)-loaded TiO2 nanocrystals (NCs) with various concentrations of Ag were prepared by soft chemical route and the effect of Ag nanoparticles (NPs) on the functional properties of TiO2 was studied. X-ray diffraction (XRD) and Raman studies confirmed that the synthesized product had single-phase nature and high crystalline quality. The crystallite size was decreased from 18.3 nm to 13.9 nm with the increasing in concentration of Ag in TiO2 NCs. FESEM micrographs showed that the pure and AgNPs-loaded TiO2 have spherical morphology and uniform size distribution with the size ranging from 20 to 10 nm. Raman spectroscopy performed on pure and AgNPs-loaded TiO2 confirms the presence of anatase phase and AgNPs. Optical properties show the characteristics peaks of TiO2 and the shifting of the peaks position was observed by changing the concentration of Ag. The tuning of bandgap was found to be observed with the increase in Ag, which could be ascribed to the synergistic effect between silver and TiO2 NCs. Density functional theory calculations are carried out for different Ag series of doped TiO2 lattices to simulate the structural and electronic properties. The analysis of the electronic structures show that Ag loading induces new localized gap states around the Fermi level. Moreover, the introduction of dopant states in the gap region owing to Ag doping can be convenient to shift the absorption edge of pristine TiO2 through visible light.

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“…4 a ). Absorption peaks at 534, 585, 520 and 579 cm −1 for both ATR‐FTIR spectra of pellet and supernatant confirm the presence of AgNP in the samples [36]. Nevertheless, the presence of 534 cm −1 peak in supernatant indicated major fraction of AgNP population partition into supernatant, upon centrifugation of cell lysate.…”

Section: Resultsmentioning

confidence: 96%

Biofabrication of silver nanoparticles using bacteria from mangrove swamp

Sharma

Nayak

Asthana

et al. 2018

IET nanobiotechnol.

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The last decade has observed a rapid advancement in utilising biological system towards bioremediation of metal ions in the form of respective metal nanostructures or microstructures. The process may also be adopted for respective metal nanoparticle biofabrication. Among different biological methods, bacteria-mediated method is gaining great attention for nanoparticle fabrication due to their eco-friendly and cost-effective process. In the present study, silver nanoparticle (AgNP) was synthesised via continuous biofabrication using , isolated from swamp wetland of Sunderban, West Bengal, India. The biofabricated AgNP was further purified to remove non-conjugated biomolecules using size exclusion chromatography, and the purified AgNPs were characterised using UV-visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy and transmission electron microscopy (TEM). Additionally, the presence of proteins as capping and stabilising agents was confirmed by the amide-I and amide-II peaks in the spectra obtained using attenuated total reflection Fourier transform infrared spectroscopy. The size of biofabricated AgNP was 10-20 nm, as observed using TEM. Additionally, biofabricated AgNP shows significant antibacterial potential against and . Hence, biofabricated AgNP using, which found resistant to a significant concentration of Ag ion, showed enhanced antimicrobial activity compared to commercially available AgNP.

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Biosynthesis and characterization of silver nanoparticles (AgNPs) using marine bacteria against certain human pathogens

Cited by 13 publications

References 18 publications

Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

Green Synthesis of Silver Nanoparticles Using Hypericum perforatum L. Aqueous Extract with the Evaluation of Its Antibacterial Activity against Clinical and Food Pathogens

An Experimental and Theoretical Study on the Effect of Silver Nanoparticles Concentration on the Structural, Morphological, Optical, and Electronic Properties of TiO2 Nanocrystals

Biofabrication of silver nanoparticles using bacteria from mangrove swamp

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